Abstract: he F1 hybrid seeds of Den. nobile♀ × Den. moniliforme♂ were taken as materials to explore in vitro rapid propagation techniques and flowering. The results showed that the optimum media for seedlings germination was 1/2MS + 6-BA 1.0 mg·L-1 + NAA 2.0 mg·L-1+ IBA 2.0 mg·L-1+ banana mud 100 g·L-1. The optimum medium for proliferation of subculture was 1/2MS + 6-BA 0.2 mg·L-1+ NAA 1.0 mg·L-1 + banana mud 100 g·L-1 with the proliferation coefficient of 4—5 times. The medium for regeneration of adventitious buds was 1/2MS + 6-BA 2.0 mg·L-1+ NAA 0.2 mg·L-1+ peptone 2 g·L-1 with 93.3% induction rate and 3.7 times induction coefficient. The rooting medium was TH + 6-BA 0.5 mg·L-1+ NAA 1.0 mg·L-1, the rooting rate was up to 91.7% with 3—5 roots. The medium for the floral bud induction was 6-BA 2.0 mg·L-1 + NAA 0.5 mg·L-1 + IBA 0.5 mg·L-1 with the rate of 8%.

Key words: Den. nobile, hybridization, rapid propagation in vitro, flowering in vitro

摘要： 以金钗石斛♀×细茎石斛♂杂交F1代种子为材料，探索两种石斛杂交后代的快繁技术和诱导试管开花。结果表明，适于种子萌发的培养基为1/2MS + 6-BA 1.0 mg·L-1 + NAA 2.0 mg·L-1 + IBA 2.0 mg·L-1 +香蕉100 g·L-1；继代增殖培养基为1/2MS + 6-BA 0.2 mg·L-1 + NAA 1.0 mg·L-1 +香蕉100 g·L-1，增殖系数达4～5倍；不定芽诱导培养基为1/2MS + 6-BA 2.0 mg·L-1 + NAA 0.2 mg·L-1 +蛋白胨2 g·L-1，诱导率达93.3%，诱导系数达3.7；生根培养基为TH + 6-BA 0.5 mg·L-1 + NAA 1.0 mg·L-1，生根率可达91.7%，根数3～5条；花芽诱导培养基为6-BA 2.0 mg·L-1 + NAA 0.5 mg·L-1 + IBA 0.5 mg·L-1，花芽诱导率达8%。

关键词: 石斛, 杂交, 离体快繁, 试管开花