The Evaluation of Antioxidant Activity of Polyphenol Compounds Using ORAC

doi:10.3969/j.issn.1009-7791.2016.03.006

›› 2016, Vol. 45 ›› Issue (03): 225-229.DOI: 10.3969/j.issn.1009-7791.2016.03.006

• Plant physiology, biochemistry and molecular biology • Previous Articles Next Articles

The Evaluation of Antioxidant Activity of Polyphenol Compounds Using ORAC

CHANG Qiang,SU Ming-hua,CHEN Qing-xi,ZENG Bi-yu,WANG Wei,LI Hui-hua

(1.College of Horticulture, Fujian Agricultural and Forestry University, Fuzhou 350002, Fujian China; 2.Fujian Key Laboratory of Physiology and Biochemistry for Subtropical Plant, Fujian Institute of Subtropical Botany, Xiamen 361006, Fujian China)

Received:2016-08-04 Revised:2016-10-12 Online:2016-09-10 Published:2016-09-10
Contact: SU Ming-hua; CHEN Qing-xi

基于ORAC评价多酚类化合物抗氧化活性

常强,苏明华,陈清西,曾碧玉,王伟,李惠华

(1.福建农林大学园艺学院，福建福州 350002；2.福建省亚热带植物研究所, 福建省亚热带植物生理生化重点实验室，福建厦门 361006)

通讯作者: 苏明华;陈清西
作者简介:常强，博士研究生，从事植物天然产物开发研究。
基金资助:
厦门市科技计划资助项目(3502Z20142001)

Abstract

Abstract: The oxygen radical absorbance capacity (ORAC) method was validated, and then ORAC values of selected 20 phenolic compounds were determined. The results indicated that linearity (R2 =0.997), limit of detection and limit of quantification (0.5—3.1 μmol·L-1), precision (＜18%), accuracy (91%—105%) were determined. And ellagic acid and gallic acid had strong antioxidant activity in p-hydroxy benzoic acid compounds. Caffeic acid and its derivatives had highest antioxidant activity in hydroxy cinnamic acid. In the flavonoid constituents, flavan-3-ols showed good antioxidant properties, followed by flavonol. ORAC was considered as simple, efficient detection method of standardization for evaluation of antioxidant activity of the phenolic compounds.

Key words: oxygen radical absorbance capacity, antioxidant activity, polyphenols

摘要： 采用氧自由基清除能力(ORAC)方法考察20种多酚类化合物的抗氧化活性。结果表明，该方法具有较优的线性关系(R2=0.997）；检测限(LOD）和定量限(LOQ）为0.5～3.1 μmol·L-1，精密度＜18%，准确度91%～105%。比较20种多酚类化合物抗氧化活性，对羟基苯甲酸类化合物中，鞣花酸和没食子酸具有较强的抗氧化活性；对羟基肉桂酸类以咖啡酸及其衍生物抗氧化活性最高；在类黄酮组分中，黄烷-3-醇表现出优良的抗氧化特性，黄酮醇次之。ORAC可作为评价多酚类化合物抗氧化的简便、高效的标准化检测方法。

关键词: 氧自由基清除能力, 抗氧化活性, 多酚

CLC Number:

Q946-33

CHANG Qiang,SU Ming-hua,CHEN Qing-xi,ZENG Bi-yu,WANG Wei,LI Hui-hua. The Evaluation of Antioxidant Activity of Polyphenol Compounds Using ORAC[J]. , 2016, 45(03): 225-229.

常强,苏明华,陈清西,曾碧玉,王伟,李惠华. 基于ORAC评价多酚类化合物抗氧化活性[J]. , 2016, 45(03): 225-229.

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The Evaluation of Antioxidant Activity of Polyphenol Compounds Using ORAC

基于ORAC评价多酚类化合物抗氧化活性

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