Subtropical Plant Science ›› 2018, Vol. 47 ›› Issue (1): 81-83.DOI: 10.3969/j.issn.1009-7791.2018.01.017

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Studies on Antibrowning in Tissue Culture of Malania oleifera

TAO Jun-feng,SU Juan,CHAO Jing-jing,YUE Jian   

  1. (Yunnan Shanlihong Biotechnology Co. Ltd., Kunming 650200, Yunnan China)
  • Received:2017-10-13 Revised:2017-11-19 Online:2018-03-30 Published:2018-03-30
  • Contact: YUE Jian

蒜头果组培褐化控制技术研究

陶俊锋,苏娟,钞晶晶,岳健   

  1. (云南山里红生物科技有限公司,云南 昆明 650200)
  • 通讯作者: 岳健
  • 作者简介:陶俊锋,硕士,从事植物育种和资源创新研究。

Abstract: Antibrowning in tissue culture of Malania oleifera was carried out by orthogonal experimental design. It was found that explant was the key factor of browning in four factors (explant, antioxygen, sterilization method, medium), and adding 2 g稬-1 PVP in medium was conductive to reduce browning rate. Using the semi-lignification shoots as explants, sterilizing with 0.1% HgCl2 and 2% NaClO, adding 2 g稬-1 PVP into medium (1/2WPM + 2.0 mg稬-1 6-BA + 0.2 mg稬-1 NAA + 30 g稬-1 sugar + 6 g稬-1 agar + 1 g稬-1 AC, pH = 5.5) were the optimum methods for browning decrease.

Key words: Malania oleifera, tissue culture, orthogonal experimental design, browning

摘要: 采用正交试验设计,研究蒜头果Malania oleifera组织培养过程中的褐化发生和控制措施。结果表明,引起蒜头果褐化的因素主次顺序为材料类型>抗氧化剂类型>消毒方式>培养基类型,选择半木质化的当年生新枝作为外植体是减轻褐化的关键,培养基中添加2 g·L-1 PVP 能有效控制褐化产生。控制褐化并提高获得率的最佳方法为采用半木质化新枝为外植体,并以0.1%升汞6 min + 2%次氯酸钠10 min消毒,在含2 g·L-1 PVP 的1/2WPM培养基中培养。

关键词: 蒜头果, 组织培养, 正交试验设计, 褐化

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