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30 March 2018, Volume 47 Issue 01 Previous Issue    Next Issue
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Plant physiology, biochemistry and molecular biology

Plant ecology, resources and taxonomy

Plant ecology, resources and taxonomy

Plant ecology, resources and taxonomy

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Effect of Silver Needle Tea Extract on Lipid Accumulation in LO2 Cells and Its Mechanism LIN Qiu-xiang,CHEN Xi,XIE Jian-wei,LIU Qian-gang 2018, 47(1): 1-7.  DOI: 10.3969/j.issn.1009-7791.2018.01.001 Abstract ( )   PDF (1911KB) ( )   In this paper, the aqueous extract of silver needle tea was used as the experimental preparation for study of its effect and mechanism on the lipid accumulation in LO2 cells. The cell model of non-alcoholic fatty liver was established by the treatment of oleic acid, and the therapeutic group, preventive group, simvastatin group and natural recovery group were arranged and respectively applied to the above experimental preparations and detected for triglycerides (TG) and malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, lipid accumulation and SREBP-1c, FAS and CPT1A mRNA expressions in LO2 cells. In this investigation, 1 mmol·L-1 oleic acid treatment for 24 h was the optimal condition for establishment of non-alcoholic fatty liver cell model, the contents of TG and MDA in the model group were higher than that in the normal group, while the SOD activity was lower when compared with the normal group, and among the groups the treatment of 1 mg·mL-1 aqueous extract in therapeutic group showed the lowest MDA content, the lowest lipid accumulation，the lowest relative expression of SREBP-1c and FAS，but the highest SOD activity and the highest relative expression of CPT1A of LO2 cells, and was regarded as the optimal aqueous preparation for prevent and treat the non-alcoholic fatty liver. The lipid accumulation of LO2 cells was significantly reduced by the treatment of the 1 mg·mL-1 aqueous extract, which may be related to the inhibition of SREBP-1c and the successive FAS gene expression, the decrease of TG synthesis, the acceleration of CPT1A gene expression and decomposition of TG, etc. References | Related Articles | Metrics

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Effects of Brassinosteroids on Germination and Seedling Growth of Aged Rice Seed CHEN Liang-liang,LI Mo-li,LUO Wei-gui,LIU Qing,SU Yi,XIAO Lang-tao 2018, 47(1): 8-12.  DOI: 10.3969/j.issn.1009-7791.2018.01.002 Abstract ( )   PDF (898KB) ( )   With aged seeds of Zhuliangyou 819, an early season hybrid rice, as material, the effects of brassinosteroids on aged seed activity and seedling growth of rice were carried out. The results showed that brassinolide treatment restored the vigor of the aged seeds in a certain degree. In comparison with the control, both the seed-soaking treatments with brassinolide prior to germination and the brassinolide treatment after germination significantly increased the germination potential and germination rate of aged rice seeds, resulting in shorter germinating time. From the prospective of seedling growth, the seed-soaking treatment with brassinolide prior to germination remarkably promoted the germ outgrowth and the radicle elongation, by improving uniform seedling growth. Brassinolide treatment after germination played a positive role in the radicle growth but an inhibitive role in germ growth. References | Related Articles | Metrics

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Assessing Adaptability of Camellia euphlebia under Different Light Intensity XUE Ke-na,XUAN Zu-ying,CHEN Si-ning,WEN Shan-na,HUANG Yong-fang 2018, 47(1): 13-17.  DOI: 10.3969/j.issn.1009-7791.2018.01.003 Abstract ( )   PDF (395KB) ( )   To assess adaptability of Camellia euphlebia seedlings at different light intensity, 2-year old cutting seedlings were used to test physiological indexes such as contents of chlorophyll, contents of soluble protein and sugar, malondialdehyde content(MDA), nutrients(N, P, K) and antioxidant activities of SOD and POD. The results showed that with the increase of light intensity, contents of chlorophyll a, b and total chlorophyll were decreased, and there was significant difference among different light intensity, while difference of chlorophyll a/b was not significant. The antioxidant activities of SOD were increased with the increased of light intensity, and SOD activity with full light treatment was significantly different from 10% and 20% light intensity treatments (P<0.05). POD activity of 40% light intensity was significantly higher than that of 10% light intensity (P<0.05). The content of MDA of full light treatment was significantly higher than the other treatments (P<0.05). Content of N of full light was significantly higher than those of 20% and 40% light intensity (P<0.05), Contents of P were significantly different among different treatments (P<0.05), but the difference of K contents was not significant. There was significant difference of soluble protein content among different light intensity (P<0.05), while the difference of soluble sugar content was not significant. Net growth of height and ground diameter of C. euphlebia seedling under 20% light intensity were highest, which showed that the seedlings grew better under 20% light intensity. References | Related Articles | Metrics

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Phylogenetic Analysis of Diaphorina citri from Southern China Based on COI Gene SONG Zhang-rong,LI Yi-han,OU Da,QIU Bao-li,SANG Wen 2018, 47(1): 18-22.  DOI: 10.3969/j.issn.1009-7791.2018.01.004 Abstract ( )   PDF (487KB) ( )   In order to provide references for an in-depth understanding of genetics and evolution on the citrus psyllid in southern China, mitochondrial cytochrome coxidase I (COI) genes of 5 geographic populations were cloned, and genetic distance and phylogeny were analyzed. The results showed that 5 geographic populations had a very closely genetic relationship (0.0%—0.2%). The genetic distance of Guangzhou, Zhanjiang populations was 0. The genetic distance among Xiamen, Guilin and Nanning populations was also 0. The phylogenetic analysis based on COI showed that genetic differentiation between the five populations in southern China was low. In addition, there was no obvious correlativity between the geographic distance of populations and the level of homology distance comparing with other 21 populations in other districts. References | Related Articles | Metrics

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Anatomical Features of Vegetative Organs of Acorus calamus and Their Ecological Adaptability SHOU Jia-xin,WU Xiao,GUO Chang-yi,YING Jian,CHEN Mo-shun 2018, 47(1): 23-27.  DOI: 10.3969/j.issn.1009-7791.2018.01.005 Abstract ( )   PDF (2559KB) ( )   The anatomical structure characteristics of vegetative organs of wetland plant Acorus calamus were observed by the paraffin method and tissue segregation process, and the relationship between these structure characteristics and ecological adaptability of A. calamus was also discussed. The experimental results showed that A. calamus rhizome had adventitious roots. Radical epidermis was composed of a layer of cells, arranging compactly. The cortex was composed of the multilayer parenchymal cells, which were ruptured to form numerous aerenchyma. The endodermis had the thickened casparian band with horseshoe in cytoderm. The vessel column had 5 prototype primary xylems and 4—10 large-scale vessel to form the developed aerenchyma. The epidermis cells out of the rhizome were squared. The extine was thickened. The elementary tissue approximating to the epidermis had the sclerenchyma cell groups. The cortex parenchymal cells were arranged in chains. There was the large aerenchyma in the middle. The endodermis formed the Casparian strip. Lots of vessels were attached around the Casparian strip. Vascular bundles were scattered in the ground tissue. The central cylinder’s vascular bundle was presented in the amphivasal type. Partial region of rhizome was mutualized with endophyte to form nodulation. The leaves of A. calamus were isolateral. The parenchymal cells of mesophyll tissues were ruptured to form the large aerenchyma. The vein had the limited collateral vascular. There was a large vessel in the middle. The anatomical structure features of A. calamus showed that it was adaptive to the growth environment of wetland. A. calamus rhizome had the endophyte in nodulation, which could purify sewage. The developed adventitious root was suitable for division propagation. References | Related Articles | Metrics

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Plant Resources Nearby the Lime Cave with Distribution of Primulina tabacum at Zhaibeilei Village of Lianzhou, Guangdong LI Chun-bo,ZHAO Xin-jian,MIAO Shen-yu,DAI Wen-tan,TAO Wen-qin 2018, 47(1): 28-32.  DOI: 10.3969/j.issn.1009-7791.2018.01.006 Abstract ( )   PDF (385KB) ( )   The investigation of plant resources were carried out nearby a lime stone cave at Zhaibeilei, Lianzhou, Guangdong, where the key national protected species of grade I (Primulina tabacum) and II (Fagopyrum dibotrys and Glycine soja) grew naturally. There were 107 species of wild vascular plants belonging to 59 families and 96 genera nearby the cave of Zhaibeilei. The plant resources were classified to 14 groups, the medicinal plant resources had the maximum percentage of 57.0% to the total. The plant resources at Zhaibeilei and its nearby area Shangbaichang with Primulina tabacum distribution had own specific characteristics. It was proposed that the lime cave area at Zhaibeilei should be strictly protected as a part of the Tianxin Provincial Nature Reserve of Lianzhou, Guangdong, as well as a lime stone cave at Shangbaichang. References | Related Articles | Metrics

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Wild Cover Plant Resources and Their Landscape Application in Qingyun Mountain Nature Reserve of Wengyuan, Guangdong Province LI Qun,CHEN Xiao-xi,ZENG Xian-xing,XIE Chong-lin,YANG Xin-dong,HUANG Jiu-xiang 2018, 47(1): 33-37.  DOI: 10.3969/j.issn.1009-7791.2018.01.007 Abstract ( )   PDF (1659KB) ( )   Based on the investigation of wild plants in Qingyun Mountain Nature Reserve, 173 species of wild cover plants belonging to 139 genera in 79 families were selected out because of their ornamental value, including 35 species of ferns and 138 species of seed plants. According to life forms, these cover plants were classified into 3 types, namely 126 herbal plants, 24 shrub plants, and 23 liana plants; according to ornamental characteristic, they were classified into 3 types, flowering plant, foliage plant, and fruit plant; according to application form in garden, they could be divided into 4 types, open ground cover, slope being, edge forest and woodland, forest. In addition, rational suggestions on protection and developmental utilization were proposed. References | Related Articles | Metrics

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Introduction and Comprehensive Evaluation of Wetland Ornamental Plants in Guangzhou HE Zhong-jian,ZHANG Ji-fang,CHEN Ying,FENG Yi-min,HE Man-mei 2018, 47(1): 38-42.  DOI: 10.3969/j.issn.1009-7791.2018.01.008 Abstract ( )   PDF (390KB) ( )   Based on introduction and collection of wetland ornamental plants in Guangzhou, a comprehensive evaluation model with 14 evaluation indices was established by Analytic Hierarchy Process (AHP). The results showed that ornamental period, stress resistance and maintenance management were the major factors affecting application value of wetland ornamental plants. Ninety-six wetland plants were classified into four grades. The gradeⅠ(≥8.5) including 9 kinds of plants, had the highest comprehensive application value, 30 kinds of plants in the grade Ⅱ(8.0~8.5) had the higher application value. References | Related Articles | Metrics

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The Status Analysis on Conservation of Ericaceae Species in Wutong Mountain National Park, Shenzhen JING Hui-juan,WANG Ding-yue 2018, 47(1): 43-47.  DOI: 10.3969/j.issn.1009-7791.2018.01.009 Abstract ( )   PDF (430KB) ( )   There were 4 species of Ericaceae in Wutong Mountain National Park, Rhododendron moulmainense, R. farrerae, R. simsii, and Enkianthus quinqueflorus. Wutong Mountain was rich in resources of azalea plants, and had unique Ericaceae species forest. But under natural conditions, the dominant species of Ericaceae was in degraded state, and the landscape effect was poor. Conservation of Ericaceae species resources were carried out since 2006. The intermediate cuttings and pruning for other dominant trees, applying fertilization management, artificial breeding, and reinforce the prevention of plant diseases and insect pests were effective measures to tending Ericaceae species resources. The artificial tending to Ericaceae species resources was a sustained mission, which need to follow the principles of protecting the community ecosystem. Of course, it was necessary to establish a sound ecological landscape forest monitoring and management information system. References | Related Articles | Metrics

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Investigation and Analysis of Flower Border Plants in Shenzhen ZHANG Wei,FU Hong-lun,WANG Lei,QIAN Mei,LIU Lu-lu,LIAO Wen-bo 2018, 47(1): 48-53.  DOI: 10.3969/j.issn.1009-7791.2018.01.010 Abstract ( )   PDF (470KB) ( )   Based on the investigation of 38 main flower borders in Shenzhen, China, it included 185 species of ornamental plants, belonging to 161 genera in 101 families. According to composition of species, application frequency, seasonal, and native plants applications, the problems in application of flower border plants were analyzed, and the amendment methods were put forward. References | Related Articles | Metrics

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Investigations on Medicinal Plant Resources in Quangang District, Fujian Province AN Chang,CAI Da-li,YANG Cheng-zi,WU Shu-ying,WEN Xiu-ping,YU Hong-min 2018, 47(1): 54-58.  DOI: 10.3969/j.issn.1009-7791.2018.01.011 Abstract ( )   PDF (446KB) ( )   To understand the present status of medicinal plant resources in Quangang District in Fujian province, the resource of medicinal plants in Quangang was investigated through field investigation, specimen collection, market research. Results showed that Quangang had 1166 species medicinal plants, belonging to 196 families. There were 58 species key varieties and 49 species traditional Chinese medicines in Fujian. The medicinal plants resources in Quangang were abundant, but the development and utilization rate of medicinal plants was low. References | Related Articles | Metrics

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The Rivalrousness of 8 Common Plants Used in Extensive Green Roof LIANG Qiong-fang,LEI Jiang-li 2018, 47(1): 59-61.  DOI: 10.3969/j.issn.1009-7791.2018.01.012 Abstract ( )   PDF (571KB) ( )   Eight species of experimental plants used in extensive green roof were planted randomly on different thickness substrates. The coverage degree of each plant and plantation landscape were investigated in order to analyze the rivalrousness among tested materials. The results showed that Portulaca grandiflora had stronger aggressiveness, and should be carefully used in extensive green roof. S. sarmentosum and S. mexicanum had strong competitiveness and resistance, and their coverage rates were increase with the decrease of substrate thickness. Competiveness of S. acre and S. emarginatum were second, and their coverage rates didn’t change much with thickness of substrates change; Kalanchoe fedtschenkoi, S. kamtschaticum ‘ellacombeanum’ and S. nussbaumerianum were suitable for vertical landscape construction. Consideration of plantation stability and construction cost, 10 cm substrate thickness was suitable for plantation landscape of extensive green roof. References | Related Articles | Metrics

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Relationships Between Land Surface Temperature and Land Cover in Main Urban Areas of Kunming Using Remote Sensing MAO Yang,BAI Yang,LIU Wen-jun,MA Bin,YANG Jian-bo 2018, 47(1): 62-68.  DOI: 10.3969/j.issn.1009-7791.2018.01.013 Abstract ( )   PDF (990KB) ( )   With Landsat 8 two images of Kunming urban area as the data source, normalized difference vegetation index (NDVI), modified normalized water index (MNDWI), impervious surface area (ISA) and thermal field variation index to reflect the change of heat island effect in different seasons were carried out. The results showed that: (1) Using the improved single window-algorithm to calculate the land surface temperature (LST) was effective. (2) The heat island effect existed spatio-temporal differences, and there was stronger effect and more concentrated distribution in May than in February. And the heat island effect level had a close relationship with land use types, building type with the strongest heat island had the maximal proportion in two months, the proportion of covering with water with strongest heat island was minimum. The燚ian燣ake had no heat island effect for its big water area. (3) The correlation between LST and surface parameters in February and May had the same trend, significant positive correlation with the ISA, and significant negative correlation with NDVI and MNDWI. The correlation between LST and NDVI, ISA in May were higher than in February, while the correlation between LST and MNDWI in February was higher than in May. (4) The urban green space and water could cool the surface temperature of the city, protecting the Dian Lake and increasing the vegetation coverage moderately will play a positive role in suppression of the urban heat island effect. References | Related Articles | Metrics

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Changes of Aeroanion concentration on the Greenway in Sanming, Fujian ZHAO Kai 2018, 47(1): 69-71.  DOI: 10.3969/j.issn.1009-7791.2018.01.014 Abstract ( )   PDF (319KB) ( )   Based on the measurement of air anion, the changes of air anion of the greenway in Sanming City were studied. The results showed that the concentration of air anion in different planting structure was tree shrub lawn＞shrub lawn＞lawn＞contrast. The concentration of air anion in different waterscape was waterfall＞rivulet＞backwater. The concentration of air anion in core area of waterfall was high in the morning and low in the afternoon. The concentration of air anion in core area of waterfall was more than 10 000 ions·cm-3. References | Related Articles | Metrics

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New Records of Seed Plants from Qiyunshan Nature Reserve, Jiangxi Province FENG Lu,WANG Hao-wei,XIAO Min,LIU Nan-nan,WANG Lei,ZHAO Wan-yi,LIAO Wen-bo 2018, 47(1): 72-76.  DOI: 10.3969/j.issn.1009-7791.2018.01.015 Abstract ( )   PDF (2701KB) ( )   Three species were reported as new records of seed plants to Jiangxi Province, China, i.e. Burmannia championii Thw., Lindernia macrobotrys Tsoong and Oreocharis curvituba J. J. Wei & W. B. Xu. And four species were reported as new records of seed plants to Qiyunshan Nature Reserve, i.e. Burmannia nepalensis (Miers) Hook. f., Oreocharis magnidens Chun ex K. Y. Pan, Goodyera yangmeishanensis T. P. Lin, Peristylus densus (Lindl.) Santap. et Kapad. References | Related Articles | Metrics

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Tissue Culture and Rapid Propagation of Spiranthes sinensis L? Ya-feng,GUO De-zhang,WEI Guang-biao 2018, 47(1): 77-80.  DOI: 10.3969/j.issn.1009-7791.2018.01.016 Abstract ( )   PDF (799KB) ( )   The effects of different disinfection treatment and the combinations of plant growth regulators on Spiranthes sinensis tissue culture with bud and inflorescence as explants were studied, and the rapid propagation system for tissue culture was established. The results indicated that the appropriate method for disinfection treatment on explants was in 0.1% mercuric chloride for 6 min. The optimal induction medium for multiple buds formation and callus induction was 1/2MS + 6-BA 2.5 mg稬-1 + NAA 0.25 mg稬-1. The optimum medium for proliferation was 1/2MS + 6-BA 2.0 mg稬-1 + NAA 0.75 mg稬-1. The optimum rooting medium was 1/2MS + NAA 1.25 mg稬-1. References | Related Articles | Metrics

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Studies on Antibrowning in Tissue Culture of Malania oleifera TAO Jun-feng,SU Juan,CHAO Jing-jing,YUE Jian 2018, 47(1): 81-83.  DOI: 10.3969/j.issn.1009-7791.2018.01.017 Abstract ( )   PDF (346KB) ( )   Antibrowning in tissue culture of Malania oleifera was carried out by orthogonal experimental design. It was found that explant was the key factor of browning in four factors (explant, antioxygen, sterilization method, medium), and adding 2 g稬-1 PVP in medium was conductive to reduce browning rate. Using the semi-lignification shoots as explants, sterilizing with 0.1% HgCl2 and 2% NaClO, adding 2 g稬-1 PVP into medium (1/2WPM + 2.0 mg稬-1 6-BA + 0.2 mg稬-1 NAA + 30 g稬-1 sugar + 6 g稬-1 agar + 1 g稬-1 AC, pH = 5.5) were the optimum methods for browning decrease. References | Related Articles | Metrics

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Cutting Propagation Technology of Sarcandra glabra HUANG Yi-cheng,ZHENG Hai,ZENG Qing-qian,YUAN Liang,MO Huo-yue 2018, 47(1): 84-87.  DOI: 10.3969/j.issn.1009-7791.2018.01.018 Abstract ( )   PDF (354KB) ( )   Using rooting rate, root number, root length as valuation indexes, the effects of substrates, cutting positions and growth regulators on cutting propagation of Sarcandra glabra were investigated by orthogonal experimental design. The results showed that cutting position type was the key factor affecting cutting rooting, and substrate type was taking second place. However, IBA had inhibiting effect on cutting rooting, NAA had no effect on rooting of cutting. The optimum treatment for rooting of Sarcandra glabra cuttings were using top cutting and taking loess as substrate with rooting rate 98.12%, rooting number 12.11 per plant and average root length 8.31 cm. References | Related Articles | Metrics

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Research Progress and Prospective of Stress Resistance of Blueberry Improved by Mycorrhizal Fungi CHEN Meng,SU Dong-shan,ZHU Hong-hui,CHEN Jie-zhong,YAO Qing 2018, 47(1): 88-93.  DOI: 10.3969/j.issn.1009-7791.2018.01.019 Abstract ( )   PDF (5061KB) ( )   Ericoid mycorrhizal fungi (ErMF) and arbuscular mycorrhizal fungi (AMF) play vital roles in promoting growth and stress resistance of blueberry Vaccinium. This paper reviewed the current status of blueberry production, the properties of blueberry and ErMF & AMF, the influences of ErMF & AMF on stress resistance of blueberry. Prospective on stress resistance of blueberry improved by ErMF & AMF was presented. References | Related Articles | Metrics

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The Development Process of DNA Sequencing Technology MAO Ya-wen,CHEN Jiang-hua 2018, 47(1): 94-100.  DOI: 10.3969/j.issn.1009-7791.2018.01.020 Abstract ( )   PDF (384KB) ( )   In view of DNA sequencing technology developing, bioinformatics, molecular genetics, precision medicine and genomics were developing and supporting in-depth understanding of life. In this review, several generations of sequencing technology were introduced, including their advantages and disadvantages and the future development of sequencing technology. References | Related Articles | Metrics