Abstract: A high-performance liquid chromatography method for determination of resveratrol was developed. The determination of resveratrol was performed on a Hypersil C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase was methanol-water (72:28), and the flow rate was 1 mL·min-1. The detector was photodiode array detector and the variable wavelength detector was set at 306 nm. In the above chromatographic conditions, good linear was observed in the linear range of 10～200 μg·mL-1, and the correlation coefficient (r) was 0.9999. The relative standard deviation (RSD) of the precision, reproducibility and stability were 0.77%, 0.41% and 0.21%, respectively. The average recovery of standard addition was 99.92% with RSD of 0.39%. The method is sensitive, reliable, accurate, and could be used for the determination of resveratrol.

Key words: HPLC, Vitis thunbergii var. taiwaniana, resveratrol, determination

摘要： 建立一种高效液相色谱法(HPLC)测定小叶山葡萄Vitis thunbergii var. taiwaniana叶中白藜芦醇含量的检测方法。Waters高效液相色谱仪：Hypersil C18色谱柱(250 mm × 4.6 mm，5 μm)；流动相：甲醇-水(72:28)；流速：1 mL·min-1；检测波长306 nm。结果表明，在上述色谱条件下，白藜芦醇含量在10～200 μg·mL-1范围内线性关系良好，相关系数r为0.9999。精密度、重现性、稳定性的RSD(n=5)分别为0.77%、0.41%、0.21%；平均加标回收率为99.92 %，相对标准偏差为0.39 %。该方法准确、灵敏、可靠，可用于白藜芦醇的定量和定性分析。

关键词: HPLC, 小叶山葡萄, 白藜芦醇, 测定