Two-dimensional Liquid Chromatography for the Identification of Euscaphis japonica var. wupingensis and E. japonica

doi:10.3969/j.issn.1009-7791.2020.06.011

Subtropical Plant Science ›› 2020, Vol. 49 ›› Issue (06): 469-472.DOI: 10.3969/j.issn.1009-7791.2020.06.011

• Plant resources and taxonomy • Previous Articles Next Articles

Two-dimensional Liquid Chromatography for the Identification of Euscaphis japonica var. wupingensis and E. japonica

HUANG Yi-qing¹, XING Yun¹, LIU Shun-zhi¹, FAN Shi-ming², CAI Bang-ping^3*, QIU Ying-kun^1*

(1.Fujian Provincial Key Laboratory of Innovative Drug Target Research, School of Pharmaceutical Sciences, Xiamen University, Xiamen 361105, Fujian China; 2.Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian China; 3.Xiamen Botanical Garden, Xiamen 361003, Fujian China)

Received:2020-11-12 Revised:2020-12-27 Online:2020-12-30 Published:2020-12-30
Contact: QIU Ying-kun

二维液相色谱识别黄果野鸦椿及其原变种

黄怡晴¹，邢云¹，刘顺治¹，范世明²，蔡邦平^3*，丘鹰昆^1*

(1.厦门大学，福建厦门 361105；2.福建中医药大学，福建福州 350122；3.厦门园林植物园，福建厦门 361003)

通讯作者: 丘鹰昆
基金资助:
第四次全国中药资源普查项目(财社[2018]43号)

Abstract

Abstract: Online two-dimensional liquid chromatography was used for the comparative composition analysis of the pericarp of Euscaphis japonica var. wupingensis and E. japonica. The results showed that the two-dimensional RPLC×RPLC chromatograms were similar intraspecies, but some components were still different. In addition, heart-cutting two-dimensional chromatography was used to localize the three differential components between these two species. This experiment provided reference for the chemical differentiation of E. japonica var. wupingensis and E. japonica.

Key words: Euscaphis japonica var. wupingensis, two-dimensional liquid chromatography, RPLC×RPLC, variety

摘要： 运用在线二维液相色谱对野鸦椿(Euscaphis japonica)及其变种黄果野鸦椿(E. japonica var. wupingensis)的果皮化学成分进行对比分析。结果表明，黄果野鸦椿及其原变种的RPLC×RPLC二维色谱分析图谱较为相似，但仍有部分成分存在差异，进一步采用中心切割二维色谱定位了黄果野鸦椿及其原种的3个差异成分，为二者的化学鉴别提供借鉴。

关键词: 黄果野鸭椿, 二维液相色谱, RPLC×RPLC, 变种

CLC Number:

Q949.755

HUANG Yi-qing, XING Yun, LIU Shun-zhi, FAN Shi-ming, CAI Bang-ping, QIU Ying-kun. Two-dimensional Liquid Chromatography for the Identification of Euscaphis japonica var. wupingensis and E. japonica[J]. Subtropical Plant Science, 2020, 49(06): 469-472.

黄怡晴，邢云，刘顺治，范世明，蔡邦平，丘鹰昆. 二维液相色谱识别黄果野鸦椿及其原变种[J]. 亚热带植物科学, 2020, 49(06): 469-472.

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Two-dimensional Liquid Chromatography for the Identification of Euscaphis japonica var. wupingensis and E. japonica

二维液相色谱识别黄果野鸦椿及其原变种

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