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30 December 2020, Volume 49 Issue 06 Previous Issue    Next Issue
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Plant biochemistry and molecular biology
Plant resources and taxonomy
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Plant biochemistry and molecular biology

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Screening of DNA Barcoding for Medicinal Dendrobium in Yunnan XU Su-su, GAO Jing, CHEN Jun-wen, LIU Xiang-yu, ZHANG Jing-li 2020, 49(06): 411-419.  DOI: 10.3969/j.issn.1009-7791.2020.06.001 Abstract ( )   PDF (493KB) ( )   Twelve common medicinal Dendrobium species in Yunnan province were selected, and molecular biology identification techniques were used to screen DNA barcoding sequences suitable for Yunnan medicinal Dendrobium. A total of 36 samples of 12 kinds of medicinal Dendrobium (Dendrobium devonianum, D. chrysotoxum, D. falconeri, etc.) were used as materials to extract the total DNA of the samples, and the nuclear gene fragments ITS and ITS2, the chloroplast gene fragment psbA-trnH and matK sequences were amplified and sequenced, part of the Dendrobium sequence was downloaded in GenBank; Sequence analysis and phylogenetic analysis were used by bioinformatics software. The results showed that the success rates of amplification and sequencing of the 4 sequences were 100% and all of them had no obvious Barcoding Gap, but there was a small amount of overlap between the ITS sequence and the ITS2 sequence within and between species, which tended to be biased towards both ends. The phylogenetic tree showed that the ITS and psbA-trnH sequences could successfully distinguish the 12 medicinal Dendrobiums in this study, the ITS2 sequence failed to distinguish D. longicornu and D. bellatulum. The matK sequence only distinguished 6 species of Dendrobium. It is suggested that ITS and psbA-trnH sequences should be regarded as the identification sequence of Yunnan medicinal Dendrobium to provide theoretical basis for the provenance identification of medicinal Dendrobium. References | Related Articles | Metrics

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Isolation and Identification of Endophytic Bacillus sp. Producing IAA from Taxus wallichiana var. mairei and Its  DONG Meng-meng, YUAN Bo, XU Ling-xia, CAO Xiao-ying, JIANG Ji-hong* 2020, 49(06): 420-426.  DOI: 10.3969/j.issn.1009-7791.2020.06.002 Abstract ( )   PDF (400KB) ( )   In this paper, the isolation and identification of endophytic bacteria from Taxus wallichiana var. mairei, screening of IAA producing strain and the production of lipopeptides by IAA producing strain were studied. Using tissue grinding method, the endophytic bacteria was isolated from fresh tissues of T. wallichiana var. mairei. Using 16S rRNA sequencing technology, the isolated endophytic bacteria was identified and analyzed. The IAA content in the extract was determined by high performance liquid chromatography-high resolution flight mass spectrometry and the lipopeptide compounds in the extract were separated and identified by high performance liquid chromatography-TOFMS. Variation of lipopeptide-producing compounds and their contents were screened in screening strains based on database. Results showed that thirty-one endophytic bacteria were isolated and identified from the rhizomes, stems and leaves of T. wallichiana var. mairei. And twenty IAA-producing strains were screened out. Among them, Bacillus strain KLBMPTC10 produced IAA as high as 210.3955 μg·L-1. In the study of lipopeptide production by IAA-producing strains, it was found that KLBMPTC10 was the most abundant lipopeptide in all IAA-producing endophytic bacteria, and its relative content of Iturin A2 was 26.06%, which was obviously superior to other IAA-producing strains. Bacillus strain KLBMPTC10 had the high ability to produce IAA and lipopeptides at the same time. It may be used as a research strain for further development and research. References | Related Articles | Metrics

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Antioxidant and Liver Protective Effect of Mentha haplocalyx and Ocimum basilicum Extracts LI Xuan, LIANG Shi, HUANG Wen, XU Chuan-jun, MING Yan-lin 2020, 49(06): 427-432.  DOI: 10.3969/j.issn.1009-7791.2020.06.003 Abstract ( )   PDF (420KB) ( )   ABTS and DPPH methods were used to evaluate antioxidant activity of Mentha haplocalyx and Ocimum basilicum extracts. Ethanol was used to induce damage of HepG2 cells. The survival rates of HepG2 cells after treatment with extracts were used to evaluate the liver protective effect. The results showed that the order of DPPH free radicals scavenging ability was ethanol extract of M. haplocalyx, water extract of M. haplocalyx, ethanol extract of O. basilicum, water extract of O. basilicum, and the EC50 values were 70.42 μg·mL-1, 75.77 μg·mL-1, 354.87 μg·mL-1 and 451.53 μg·mL-1, respectively. The order of ABTS free radicals scavenging ability was ethanol extract of M. haplocalyx, ethanol extract of O. basilicum, water extract of M. haplocalyx, water extract of O. basilicum, and the EC50 values were 9.04 μg·mL-1, 18.03 μg·mL-1, 22.18 μg·mL-1 and 36.88 μg·mL-1, respectively. The antioxidant capacity of M. haplocalyx extract was better than O. basilicum extract and the ethanol extract was better than water extract. The cell viability assay of alcoholic liver injury showed that the survival rates of the 5, 10, 20, 50 μg·mL-1 treatment groups of water extract of M. haplocalyx, ethanol extract of M. haplocalyx and water extract of O. basilicum were significantly higher than the ethanol injury group (eth) and showed significant liver protective effect. References | Related Articles | Metrics

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Acute Toxicity Study on Zebrafish of Commonly Used Soup Ingredient Ficus Plant in Southern Fujian Province WANG Ke, WU Yi-hong, LIU Shun-zhi, FAN Shi-ming, QIU Ying-kun 2020, 49(06): 433-437.  DOI: 10.3969/j.issn.1009-7791.2020.06.004 Abstract ( )   PDF (642KB) ( )   Zebrafish model was used to evaluate the acute toxicity of aqueous extracts and alcohol extracts of different medicinal parts of Ficus hirta, F. erecta var. beecheyana, F. pumila, F. variolosa. The mortality rate of fish was recorded at 48 h and 96 h after the administration of the extracts, and the embryonic malformation rate and embryonic development of each group were observed by stereomicroscopy. The results showed that the water extracts of the roots, stems, leaves, fruits and other different medicinal parts of the plants of Ficus in Moraceae were low or non-toxic at a dose of 200 μg·mL-1, and had no adverse effects on zebrafish embryos, the effect of promoting embryo growth was observed. However, the alcohol extract had a certain effect on zebrafish embryo development at high doses. The alcohol extracts from thick leaf of F. hirta, the stems and leaves of F. erecta var. beecheyana, and the leaves of F. variolosa led to the tail deformity of zebrafish embryos. The alcohol extracts from F. pumila leaves, fruit had obvious pericardial edema. The results showed that the above-mentioned medicinal materials were safe and non-toxic when used as traditional soup materials, but the dosage should be paid attention to when used as medicinal wine raw materials. References | Related Articles | Metrics

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The Chemical Constituents and Antioxidant Activities of Dendrobium officinale GENG Wen-hui, LU Shao-ji, MING Yan-lin 2020, 49(06): 438-442.  DOI: 10.3969/j.issn.1009-7791.2020.06.005 Abstract ( )   PDF (362KB) ( )   Sulfuric acid phenol method, AlCl3 method and acid dye colorimetry were used to determine the contents of polysaccharides, flavonoids and alkaloids in flowers, leaves and stems of Dendrobium officinale. DPPH and ABTS clearance test were used to evaluate the antioxidant activities in vitro of water extracts and ethanol extracts from flowers, leaves and stems of D. officinale. The results showed that the content of polysaccharide: stem>flower>leaf, flavonoid content: flower>leaf>stem. Alkaloids were less distributed in all parts. The content of flavonoids in flower was 1.847%, the content of polysaccharide in stem was 23.92%. The DPPH radical scavenging capacity was as follows: flower water extract, stem alcohol extract, flower alcohol extract, the half effect concentration (EC50) were 410.4 μg·mL-1, 454.1 μg·mL-1, 573.2 μg·mL-1, respectively. The ABTS radical scavenging ability was as follows: flower alcohol extract, flower alcohol extract, flower water extract, the half effect concentration (EC50) were 61.1 μg·mL-1, 62.2 μg·mL-1, 103.0 μg·mL-1, respectively. The antioxidant activity of D. officinale flower was better than that of leaves and stems, and the antioxidant capacity of ethanol extract was better than that of water extract. References | Related Articles | Metrics

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Contents Analysis on Total Flavonoids and Luteolin of Glossocardia bidens in Fujian Province LIN Jie-qiong，CAI Kui，SHU Yi-ping，LI Huang，YE Xiang-li 2020, 49(06): 443-446.  DOI: 10.3969/j.issn.1009-7791.2020.06.006 Abstract ( )   PDF (269KB) ( )   Determination of Trace Elements and Heavy Metal Elements in Gnaphalium affine by ICP-MS References | Related Articles | Metrics

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Contents Analysis on Total Flavonoids and Luteolin of Glossocardia bidens in Fujian Province HUANG Qiao-ting, CHEN Shu-ting, HAUNG Yi-hai, LIU Xiao-fen* 2020, 49(06): 447-451.  DOI: 10.3969/j.issn.1009-7791.2021.01.007 Abstract ( )   PDF (312KB) ( )   The contents of total flavonoids and luteolin were analyzed by UV and HPLC to learn the quality level of Glossocardia bidens which produced in Fujian Province. The results showed that the average content of total flavonoids was 32.879 mg·g-1, the sample which content of top three were from Angu village(50.986 mg·g-1), Fushan village(42.242 mg·g-1) and Houzeng village(41.076 mg·g-1), and the lowest content sample was from Nanshan village(18.096mg·g-1); The average content of luteolin was 3.695 mg·g-1, the sample which content of top three were from Tongling town 4(12.500 mg·g-1), Ao’jiao village(9.720 mg·g-1) and Angu village(9.071mg·g-1), and the lowest content sample was from Tongling town 2(0.473 mg·g-1). Considering the content of total flavonoids and luteolin, the quality of G. bidens produced in Angu Village, Hui'an County was the best, which could be rationally developed and utilized. References | Related Articles | Metrics

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Optimization of Forming Process of Anoectochilus roxburghii Granules by Box-Behnken Response Surface TANG Xu-chong, LU Shao-ji, MING Yan-lin, DENG Ai-hua, NIU Wen-jing 2020, 49(06): 452-457.  DOI: 10.3969/j.issn.1009-7791.2020.06.008 Abstract ( )   PDF (449KB) ( )   Adopt single-factor test and optimize design of response surface to optimize the best forming process of Anoectochilus granules. Taking the type of filler, the proportion of auxiliary materials, the amount of raw materials, the drying temperature and the amount of binder as the investigation factors, the total evaluation of the particle qualification rate, solubility, hygroscopicity and sensory evaluation (OD) as the evaluation index, the response surface design was used to optimize the molding process. The results showed that the best molding process as follows: lactose : dextrin=3:1, raw material ratio of 7.01%, drying temperature 50 ℃, according to this program, the prediction of particle forming rate was 87.18%, moisture absorption rate 8.22%, melting time 34.95%, sensory evaluation 81. In this experiment, the results of response surface method were reliable, which could provide a theoretical basis for the industrial production of Anoectochilus granules. References | Related Articles | Metrics

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The Preparation Technology of Artichoke Extract Granules TANG Xu-chong, LU Shao-ji, MING Yan-lin, DENG Ai-hua, NIU Wen-jing 2020, 49(06): 458-463.  DOI: 10.3969/j.issn.1009-7791.2020.06.009 Abstract ( )   PDF (379KB) ( )   To optimize the preparation conditions of artichoke extract granules by orthogonal design based on single factor experiment, the ratio of raw materials and excipients, the ratio of raw materials, the concentration of ethanol and the drying temperature were taken as the investigation factors, the particle pass rate and dissolution time were taken as the evaluation indexes, and the orthogonal test of L9(34) was used to analyze the particle pass rate and dissolution time directly and anova respectively. The best molding process: lactose:dextrin=3:1, ethanol concentration was 60%, raw material ratio was 7.0%, and drying temperature was 50 ℃. According to the verification experiment of this scheme, the particle properties were good, the qualified rate of the particles was 91.45%, the water content was 4.90%, the weight loss didn’t exceed 2.0%, and the dissolution time was 85.67 s, which all met the Chinese Pharmacopoeia(2020) specifications. The preparation process of granules optimized by orthogonal test was proved to be stable and simple, which provided a theoretical basis for the research and development of dosage form of artichoke extract and mass production of granules in industry. References | Related Articles | Metrics

Plant resources and taxonomy

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Identifiable Characteristics of Cirsium japonicum by Ultraviolet Spectrum ZHANG Jia-yuan, WANG xiao-yu, BI qin-qin, LIU zhi-yong, CHE Su-rong 2020, 49(06): 464-468.  DOI: 10.3969/j.issn.1009-7791.2020.06.010 Abstract ( )   PDF (399KB) ( )   The identifiable characteristics of Cirsium japonicum was studied by UV spectrum for a quick and accurate identification. Multi-solvent ultraviolet spectroscopy was used to analyze the difference of ultraviolet characteristics between self-collected C. japonicum from different areas of Fujian province and 18 batches of commercial C. japonicum from different manufacturers in anhydrous ethanol, chloroform and petroleum ether solvents, and the characteristics by mean plus variance of the main peak value (shoulder peak value) and the mean valley value were induced. The self-collected C. japonicum shows ‘valley-peak-valley-peak’ in anhydrous ethanol solvent: the peak value at 273±3 nm, 326±2 nm and the valley value at 250±2 nm, 298±1 nm, which have significant differential significance; In chloroform solvent, it shows ‘peak-valley-peak’: the peak value at 245± nm, 273±4 nm and the valley value at 260±3 nm, which have significant discrimination significance; In petroleum ether solvent, it shows ‘shoulder peak-valley-peak’: the peak value at 233±3 nm, 265±5 nm and the valley value at 257±5 nm, which have significant discrimination significance. The peak and valley characteristic induction of UV spectrum can be used to identify C. japonicum. References | Related Articles | Metrics

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Two-dimensional Liquid Chromatography for the Identification of Euscaphis japonica var. wupingensis and E. japonica HUANG Yi-qing, XING Yun, LIU Shun-zhi, FAN Shi-ming, CAI Bang-ping, QIU Ying-kun 2020, 49(06): 469-472.  DOI: 10.3969/j.issn.1009-7791.2020.06.011 Abstract ( )   PDF (356KB) ( )   Online two-dimensional liquid chromatography was used for the comparative composition analysis of the pericarp of Euscaphis japonica var. wupingensis and E. japonica. The results showed that the two-dimensional RPLC×RPLC chromatograms were similar intraspecies, but some components were still different. In addition, heart-cutting two-dimensional chromatography was used to localize the three differential components between these two species. This experiment provided reference for the chemical differentiation of E. japonica var. wupingensis and E. japonica. References | Related Articles | Metrics

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Identification of Angelica sinensis Radix, Angelica pubescens Radix and Levisticum officinale Radix by UV CHE Su-rong, ZHANG Jia-yuan, ZHANG Qiu-mei, LUO Yuan-zhen, HUANG Ze-hao 2020, 49(06): 473-476.  DOI: 10.3969/j.issn.1009-7791.2020.06.012 Abstract ( )   PDF (259KB) ( )   The identifiable characteristics of Angelica sinensis radix, A. pubescens radix and Levisticum officinale radix were studied by ultraviolet absorption spectrum. The UV absorption spectra of A. sinensis radix, A. pubescens radix and L. officinale radix in different polar solvents were compared by ultraviolet absorption spectra lines group (UASLG) method. There are obvious differences between A. sinensis radix and A. pubescens radix in four polar solvents, while A. sinensis radix and L. officinale radix can only be identified in anhydrous ethanol and distilled water solvents. The UASLG method can be used to identify A. sinensis radix and its confusions such as A. pubescens radix and L. officinale radix. References | Related Articles | Metrics

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Adaptability Evaluation of Germplasm Resources of Paris polyphylla var. chinensis Resources in Fujian SU Hai-lan, ZHENG Mei-xia, CHEN Hong, SHAN Ji-ping, ZHU Yu-jing 2020, 49(06): 477-484.  DOI: 10.3969/j.issn.1009-7791.2020.06.013 Abstract ( )   PDF (323KB) ( )   In order to solve the problems of mixed resources and few references in the process of planting Paridis Rhizoma in the low mountain and hilly areas of the south, this study observed the phenology, disease occurrence and agronomic characteristics of 120 Paridis Rhizoma resources collected in Fujian Province. And for the better-growing resources, the Paridis Rhizoma saponin was determined by HPLC method, and the correlation analysis and cluster analysis were performed by DPS software. The results showed that the collected resources of Paridis Rhizoma in the southwest region, including Yunnan, Guizhou and Sichuan, had incomplete growth periods in Fujian Province, and poor phenological periods and agronomic traits. Among 49 resources of Paridis Rhizoma, the content of saponins of Paridis Rhizoma in accordance with the Chinese Pharmacopoeia accounted for 55%. Paridis Rhizoma has wide variations in disease, agronomic traits and saponins content of Paridis Rhizoma. For planting heavy buildings in low mountain and hilly areas, the excellent resources of Paris polyphylla var. chinensis in the local or similar latitude, longitude and topography should be selected. The germplasm resources of P. polyphylla var. chinensis are scored based on the weight of adaptability, active ingredients and agronomic traits as the main components. The higher the total score, the more adaptable. This study provides reference for the screening and evaluation of P. polyphylla var. chinensis germplasm resources. References | Related Articles | Metrics

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Investigation and Analysis of Folk Traditional Application of Medicinal Plants in Wuyishan City WU Ai-qin, LI Xin, LI Yun-lin, YANG Cheng-zi, HUANG Xu-dong, ZHANG Jia-yuan, WANG Le, ZHUANG Yi-xue, MENG Jing 2020, 49(06): 485-489.  DOI: 10.3969/j.issn.1009-7791.2020.06.014 Abstract ( )   PDF (294KB) ( )   The characteristics of medicinal plant resources and traditional folk medicines of Wuyishan were investigated and analyzed by means of literature review, field collection and investigation, folk visits and traditional market visits. There were 1842 species of medicinal plants in Wuyishan City, belonging to 923 genera in 253 families. Among them, a total of 226 species of medicinal plants commonly used by the people were collected. After sorting and identification, the sources, medicinal parts and functions of 164 species were identified. Wuyishan has many kinds of medicinal plants and rich knowledge of folk medicine culture, which has a good prospect for further development. References | Related Articles | Metrics

Reviews

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The Role and Mechanisms of Alkaloids on Anti-arrhythmia WU Mei-fang, SHEN Rui-chi, WANG Jie 2020, 49(06): 490-499.  DOI: 10.3969/j.issn.1009-7791.2020.06.015 Abstract ( )   PDF (343KB) ( )   Alkaloids are nitrogen-containing organic compounds widely existing in organisms, especially in plants. Some alkaloids have a variety of physiological functions such as anti-arrhythmia, anti-hypertension, anti-inflammation and anti-tumor. Arrhythmia is a disease caused by abnormal electrophysiological activity in the heart rate or rhythm. It is the major cause of high cardiovascular morbidity and mortality. It is a serious threat to human health and lives in the worldwide. This paper reviewed the recent progress about the role and mechanisms of alkaloids on anti-arrhythmia. In addition, the development and utilization prospect of alkaloids were introduced. References | Related Articles | Metrics

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Degradation Characteristics and Nutrient Resource Recycling of Chinese Medicine Residue CHEN Lin-xia, SHEN Ji-chen, ZHOU Le-ling, KAI Guo-yin, SUI Ning 2020, 49(06): 500-505.  DOI: 10.3969/j.issn.1009-7791.2020.06.016 Abstract ( )   PDF (321KB) ( )   Chinese medicine residue is a kind of Chinese medicine waste produced in the process of extraction and processing of the effective ingredients. If it is used as a new resource applied in other fields, it can not only make full use of the effective ingredients in the residue, but also solve the environmental pollution problem caused by the treatment method in Chinese medicine residue. Based on the degradation characteristics of different kinds of Chinese medicine residue, this paper summarized the comprehensive utilization of nutrient resources, discussed the problems existing in treatment process and the theoretical feasible treatment methods, so that can provide reference for the resource utilization of Chinese medicine wastes and provide new ideas for the sustainable development of traditional Chinese medicine. References | Related Articles | Metrics

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Progress in the Development of Pharmacological Active Ingredients and Their Products of Crocus sativus LIU Qun, HAN Bing, SUN Cheng-tao, QIAN Jun, KAI Guo-yin 2020, 49(06): 506-512.  DOI: 10.3969/j.issn.1009-7791.2020.06.017 Abstract ( )   PDF (346KB) ( )   Crocus sativus is one of the new ‘Eight Flavors of Zhejiang’. The Health Commission’s document No. 8 of 2019 listed it as medicinal plant for homology of medicine and food. The development and utilization of C. sativus had higher range of applications and economic value which involved in many fields such as medical supplies, health products, food, spices, etc. This article investigated the domestic and foreign relevant literature and invention patents in the past ten years. The development status of C. sativus products had been summarized. It aimed to lay a theoretical foundation and scientific basis for the further excavation and utilization of the medicinal and edible value of C. sativus. References | Related Articles | Metrics

Herbal textual research

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Herbal Textual Research of Ganlan in Bencao Tujing CHEN Yu-yan, YANG Hong-xia, HUANG Ze-hao 2020, 49(06): 513-517.  DOI: 10.3969/j.issn.1009-7791.2020.06.018 Abstract ( )   PDF (824KB) ( )   Based on ancient herbal books and modern documents of Ganlan (Chinese traditional drug), a medicine recorded in Bencao Tujing, its characteristic, origin, efficacy were researched. The results showed that the original plants of Ganlan and illustration ‘Quanzhou Ganlan’ recorded in Bencao Tujing were Canarium album (Lour.) Rauesch., the original plants of ‘Bosi Ganlan’ recorded in Bencao Tujing were Canarium bengalense Roxb., while the original plants of Ganlan recorded in Zhiwu Mingshi Tukao were Elaeocarpus sylvestris (Lour.) Poir. Thus, clarifying the breeds of Ganlan in the Bencao Tujing, and some confuse variety, provided basic guarantee for the experimental research and development and application of Ganlan (Canarium album (Lour.) Rauesch.). References | Related Articles | Metrics

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Medicinal Plant Characteristics and Clinical Significance of Traditional Chinese Medicine in the Vegetable JIANG Shi-qing，HUANG Ze-hao 2020, 49(06): 518-523.  DOI: 10.3969/j.issn.1009-7791.2020.06.019 Abstract ( )   PDF (287KB) ( )   Taking the varieties included in the vegetable section of Bencao Tujing (edited edition) as the research object, this paper summarized the contents of the volume by using the methods of literature research and data statistical analysis tools. The results showed that most of the varieties recorded in this part were traditional Chinese medicine with edible and medical functions. Su Song paid special attention to recording the taboos, usage and preparation methods, and the source of medical prescriptions when recording these kinds of herbs, so as to ensure the correctness, safety and rationality of clinical medication. It can be seen that Su Song’s academic thought has a good enlightenment and guidance on modern clinical Chinese medicine. References | Related Articles | Metrics

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Section of Bencao Tujing XIA Xun-li 2020, 49(06): 524-526.  DOI: 10.3969/j.issn.1009-7791.2020.06.020 Abstract ( )   PDF (232KB) ( )   Bencao Tujing is a classic with unique literature value and writing thoughts authored by Su Song. Although Bencao Tujing was originated from the government’s initiative and organization, the medicine pictures and its text came from the hard working of the local governments and the medical laboring people, the monograph authored by Su Song obviously reflected his writing thoughts of herbal. Studying the herbal writing thoughts of Su Song is of great historical and practical significance, which could further exploring the valuable literature information contained in the Bencao Tujing and inheriting the rich medical science and living culture of traditional Chinese medicine. References | Related Articles | Metrics