Abstract: Liquidambar formosana, a representative deciduous species of the Altingiaceae, holds significant economic and ecological values. The TCP gene family plays a crucial role in plant growth, development, and adaptation to stress. In this study, we integrated the genomic and transcriptomic data of L. formosana and used bioinformatics approaches to identify the LfTCP gene family at the whole-genome level. We analyzed the physicochemical properties of the proteins, gene locations, phylogenetic and evolutionary relationships, intra- and interspecific collinearity, promoter cis-acting elements, and tissue-specific expression profiles. The LfTCP gene family consists of 22 members with protein lengths ranging from 197 to 552 amino acids. These 22 LfTCP genes are unevenly distributed across 12 chromosomes. Phylogenetic analysis indicates that there are 12 members in Class I and 10 members in Class II, including 7 members in the CIN subfamily and 3 members in the CYC/TB1 subfamily. The promoter cis-regulatory elements suggest that the LfTCP gene family may be involved in responses to light, hormones, and stress. Tissue-specific expression analysis shows that LfTCP genes exhibit distinct tissue expression patterns, with LfTCP6, LfTCP7, and LfTCP17 being specifically highly expressed in leaves. These genes can serve as important candidates for studying the growth and morphological development of L. formosana leaves. The results provide a reference for understanding the functions of the LfTCP gene family and the molecular mechanisms by which TCP regulates leaf growth and development in L. formosana.

Key words: Liquidambar formosana, TCP gene family, bioinformatics, tissue expression

摘要： 枫香树Liquidambar formosana是蕈树科落叶型代表树种，具有较高的经济价值和生态价值。TCP基因家族在植物生长发育及逆境适应中扮演着关键角色。基于枫香树基因组数据和转录组数据，利用生物信息学方法，在全基因组水平上鉴定LfTCP基因家族，并对其蛋白质理化性质、基因位置、系统发育进化关系、种内种间共线性关系、启动子顺式作用元件以及组织表达量进行分析。结果表明，枫香树TCP基因家族共有22个成员，编码蛋白长度介于197～552 AA之间；22个LfTCPs基因非均匀分布于12条染色体；LfTCPs在Class Ⅰ中有12个成员，在Class Ⅱ中有10个成员，其中CIN亚家族有7个成员，CYC/TB1亚家族有3个成员。LfTCP基因家族启动子顺式元件可能与光、激素和胁迫响应有关。LfTCPs基因具有组织表达特异性，LfTCP6、LfTCP7和LfTCP17在叶中特异性高表达，可作为研究枫香树叶片生长和形态建成的重要候选基因。该研究结果可为枫香树TCP家族基因的功能及调控机制提供参考。

关键词: 枫香树, TCP基因家族, 生物信息学, 组织表达