Auxin Signaling Pathway-Mediated Regulation of Lateral Root Formation of Maize Plants by Arbuscular 
Mycorrhizal Fungus

doi:10.3969/j.issn.1009-7791.2025.02.003

Subtropical Plant Science ›› 2025, Vol. 54 ›› Issue (2): 132-143.DOI: 10.3969/j.issn.1009-7791.2025.02.003

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Auxin Signaling Pathway-Mediated Regulation of Lateral Root Formation of Maize Plants by Arbuscular Mycorrhizal Fungus

ZHU Feng-wa¹, CHEN Wei-li^1,3, ZHANG Wei¹, CHEN Mei-zheng¹, LIU Xiao-di², YAO Qing^1,2*

(1. College of Horticulture, South China Agricultural University / Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs / Guangdong Provincial Key Laboratory of Microbial Signaling and Disease Control / Guangdong Engineering Research Center for Litchi, Guangzhou 510642, Guangdong China; 2. Institute of Microbiology, Guangdong Academy of Sciences / State Key Laboratory of Applied Microbiology Southern China / Key Laboratory of Agricultural Microbiomics and Precision Application (MARA) / Key Laboratory of Agricultural Microbiome (MARA), Guangzhou 510070, Guangdong China; 3. Tea Research Institute, Anhui Academy of Agricultural Sciences, Huangshan 245600, Anhui China)

Received:2024-11-05 Accepted:2024-12-26 Online:2025-04-30 Published:2025-06-27
Contact: YAO Qing

AM真菌介导生长素信号途径对玉米侧根形成的调控效应

朱凤娃¹，陈伟立^1,3，张微¹，陈美铮¹，刘晓迪²，姚青^1,2*

(1. 华南农业大学园艺学院 / 农业农村部华南地区园艺作物生物学与种质创制重点实验室 / 广东省微生物信号与病害防治重点实验室 / 广东省荔枝工程中心，广东广州 510642；2. 广东省科学院微生物研究所/华南应用微生物国家重点实验室 / 农业农村部农业微生物组学与精准应用重点实验室 / 农业农村部农业微生物组学重点实验室，广东广州 510070；3. 安徽省农业科学院茶叶研究所，安徽黄山 245600)

通讯作者: 姚青
基金资助:
国家自然科学基金(2077040、32200087)；广东省农业农村厅“十四五”广东省农业科技创新十大主攻方向“揭榜挂帅”项目 (022SDZG09)

Abstract

Abstract: Arbuscular mycorrhizal (AM) fungi have the capacity to alter plant lateral root (LR) formation, a process that is highly dependent on phosphorus (P) levels and plant hormones. This study using maize (Zea mays) as test plants, Rhizophagus irregularis was inoculated in soils with no P application (soil available P of 20.5 mg·kg^–1, namely low P level) and additional P application of 30 mg P·kg^–1 as KH₂PO₄ (namely high P level). Plant biomass, LR number, the contents of indole-3-acetic acid (IAA) and zeatin riboside (ZR) in roots were determined, and the expression of LR formation-related genes (ZmKRP2, ZmPSK1, and ZmPSK3) and auxin signaling-related genes (ZmNAC1, ZmARF1, ZmTIR1, ZmPIN1b, and ZmAUX1) was also quantified. At 35 days after sowing, AM fungal significantly increased maize biomass at both phosphorus levels compared with no inoculation. AM fungal inoculation significantly increased the numbers of 2^nd LR and 3^rd LR by 53.5%–237.8%, while the effect of P levels on LR number was relatively weak. AM fungal inoculation significantly enhanced the expression of ZmPSK3, and P levels enhanced the expression of ZmPSK1, ZmPSK3, and ZmKRP2. Additionally, IAA contents and signaling pathways were significantly affected by both P levels and AM fungal inoculation. When inoculated with AM fungus, IAA content at 35 days after sowing at low P level was significantly increased by 48.4% than that at high P level. AM fungal inoculation significantly upregulated the expression of auxin signaling-related genes. Compared with 1ow P level, high P level significantly increased the expression of ZmNAC1, but decreased the expression of ZmAUX1. The findings indicated that ZR content was not affected by P level and AM fungus, while the auxin signaling pathway was involved in the LR formation mediated by AM fungal inoculation, whose effect magnitude was more pronounced that that of P levels.

Key words: auxin signaling-related genes, lateral root formation, phytosulfokine, Rhizophagus irregularis, soil phosphorus level, Zea mays

摘要： 丛枝菌根(AM)真菌会影响植物侧根形成，而后者高度依赖于土壤磷水平和植物激素水平。本文以玉米Zea mays为试材，在不施磷土壤(有效磷为20.5 mg·kg^-1，低磷处理)和补充施用30 mg P·kg^-1磷肥(KH₂PO₄)的土壤(高磷处理)中接种异型根孢囊霉Rhizophagus irregularis，测定玉米植株生物量、侧根数及吲哚-3-乙酸(IAA)、玉米素核苷(ZR)含量，并对侧根发育相关基因(ZmKRP2、ZmPSK1、ZmPSK3)和生长素信号途径相关基因(ZmNAC1、ZmARF1、ZmTIR1、ZmPIN1b、ZmAUX1)表达进行定量分析。在播种35 d后，与不接种相比，AM真菌在两种磷水平下均显著增加玉米生物量；AM真菌使二级和三级侧根数显著增加53.5%～237.8%，而磷水平的影响相对较小；接种AM真菌显著增强ZmPSK3基因表达，磷水平增强ZmPSK1、ZmPSK3和ZmKRP2基因表达。此外，IAA含量和信号途径受AM真菌和磷水平的显著影响，在接种AM真菌时，播种35 d后低磷水平处理玉米IAA含量比高磷水平显著增加48.4%；接种AM真菌显著促进生长素信号相关基因表达；与低磷水平相比，高磷水平显著增强ZmNAC1表达，显著降低ZmAUX1表达。ZR含量不受磷水平和AM真菌影响；生长素信号途径参与AM真菌介导侧根形成过程的调控，且AM真菌调控效应超过了磷。

CLC Number:

Q945

ZHU Feng-wa, CHEN Wei-li, ZHANG Wei, CHEN Mei-zheng, LIU Xiao-di, YAO Qing. Auxin Signaling Pathway-Mediated Regulation of Lateral Root Formation of Maize Plants by Arbuscular Mycorrhizal Fungus[J]. Subtropical Plant Science, 2025, 54(2): 132-143.

朱凤娃, 陈伟立, 张微, 陈美铮, 刘晓迪, 姚青. AM真菌介导生长素信号途径对玉米侧根形成的调控效应[J]. 亚热带植物科学, 2025, 54(2): 132-143.

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Auxin Signaling Pathway-Mediated Regulation of Lateral Root Formation of Maize Plants by Arbuscular Mycorrhizal Fungus

AM真菌介导生长素信号途径对玉米侧根形成的调控效应

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