Abstract: The optimum conditions for the development of somatic embryogenesis and plantlet regeneration of Carica papaya cv.Suizhonghong-48 was studied. The results showed that MS medium added with 2% coconut milk, ABA at 0.1 mg/L and sucrose at 40 g/L was suitable for the development and maturity of funnel-shaped embryoids of C. papaya. The healthy plantlets were regenerated from the fully mature cotyledonous embryoids on adjusted MS medium (half strength of microelements) containing sucrose at 30 g/L and 1500 lx of illumination. The regeneration rate was 78%.

Key words: Carica papaya, somatic embryogenesis, development, regeneration

摘要： 以番木瓜(穗中红-48)漏斗型体细胞胚胎为材料,探讨体细胞胚胎发育及植株再生的适宜条件。研究结果表明,附加2%椰乳、0.1mg/L ABA及40g/L蔗糖的MS固体培养基较适合番木瓜漏斗型胚状体的发育及成熟。充分成熟的子叶型胚状体在大量元素减半、蔗糖含量30g/L的MS培养基上,配合15001x光照可再生健康小植株;再生率为78%。

关键词: 番木瓜, 体细胞胚胎, 发育, 再生