Abstract: The light green compact callus was developed from leaf explant of Petunia hybrida var. grandiflora when cultured on MS medium supplemented with 6-BA 1.0mg/L+NAA 0.1 mg/L for 3 weeks. When calli were transferred into the differentiation medium, MS+6-BA 0.5 mg/L+4-PU 0.5 mg/L+NAA 0.1mg/L for 1 week, adventitious buds were formed. Transferred the buds with 3cm high into the root induction medium (1/2MS+NAA0.1mg/L+GA₃ 0.5mg/L) for 10d, roots were induced.

Key words: Petunia hybrida, tissue culture, plant regeneration

摘要： 矮牵牛叶片外植体在MS+6-BA 1.0mg/L+NAA 0.1mg/L培养基上培养3周后产生致密的浅绿色愈伤组织;转入芽分化培养基MS+6-BA 0.5mg/L+4-PU 0.5mg/L+NAA 0.1mg/L 1周后,从愈伤组织表面不断分化产生幼芽;待幼芽长至3cm时转接至生根培养基1/2MS+NAA 1.0 mg/L+GA₃0.5mg/L中生根,长成完整植株。

关键词: 矮牵牛, 组织培养, 植株再生