Abstract: Solid-state fermentation process was carried out to convert brewer's grains to produce cellulase by Trichoderma viride Persoon ex fries.Such factors including initial moisture, fermentation time, initial pH value, the amounts of wheat bran and nitrogen source types were studied in this experiment.An optimum medium ingredient was obtained by primary test of single factor and orthogonal design experiment.The results were as follows.The best fermentation conditions were initial pH 5~6, fermentation temperature 28~30℃ and fermentation time 4d, and the best medium composition was wheat bran 30%, water contain 50% at least and (NH₄)₂SO₄ 2.5%.

Key words: Trichoderma viride, brewers, cellulase, optimum, condition

摘要： 对绿色木霉接种到啤酒糟固态发酵产纤维素酶的培养基和培养条件进行优化,考察发酵物料起始含水量、发酵时间、起始pH值等发酵条件,以及啤酒糟培养基中添加麸皮、氮源种类对产酶的影响。结果表明,以啤酒糟为发酵基质接种绿色木霉生产纤维素酶是可行的。经单因素和正交试验获得最适固态发酵的培养条件为:起始pH 5~6,培养温度28~30℃,发酵4 d;最佳发酵培养基组合为:麸皮比例30%,培养基起始含水量50%,(NH₄)₂SO₄添加量为2.0%~2.5%。

关键词: 绿色木霉, 啤酒糟, 纤维素酶, 优化条件