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10 September 2007, Volume 36 Issue 03 Previous Issue    Next Issue

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Cadmium Absorption, Distribution and Tolerance in Longan XU Wen-bao, XIE Zhi-nan, LIU Hong-zhou, ZHONG Zan-hua, CAI Xiao-dong 2007, 36(03): 1-3.  Abstract ( )   PDF (155KB) ( )   The experiment was carried out using pot-cultured three-year-old longan trees to determine the variation of cadmium (Cd) content and growth of longan plant after Cd applied to soil.The results showed that more of the absorbed Cd assembled in the roots and less transferred to the leaves and fruits.The order of Cd content from high to low in different parts of longan was root, leaves and fruit.Longan tree had strong tolerance to Cd stress.When applied Cd 400 mg/kg to soil, there was no significant toxic symptom on the plant, but the content of Cd in fruit was seriously excessive. Related Articles | Metrics

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Changes of Mineral Element Accumulation and Distribution in Young Age Strawberry under Cadmium Stress ZHOU Bi-qing, ZHANG Jin-biao, HUANG Wei-nan 2007, 36(03): 4-7.  Abstract ( )   PDF (205KB) ( )   Changes of mineral element accumulation and distribution in leaves and root of young age strawberry under cadmium stress were investigated by hydroponic experiment.The results showed that the accumulation of mineral elements in strawberry root, such as K, Ca, Mg, P, Cu, Zn Mn and Fe, was reduced under stress of high concentration cadmium.Similarly, contents of K, Ca, Mg, P, Cu, Zn, Mn, Fe, B and Mo in strawberry leaves decreased with the increase of cadmium concentration.However, contents of some mineral elements, such as K, Mg, P and Zn in both strawberry leaves and root increased at low cadmium concentration.Mineral element distribution in strawberry changed under cadmium stress.Also, contents of some mineral elements were significantly correlated to each other. Related Articles | Metrics

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Research on Photosynthetic Daily Course of Ulmus crassifolia and Its Influencing Factors XIA Shang-guang, ZHANG Jin-chi, LIANG Shu-ying 2007, 36(03): 8-11,36.  Abstract ( )   PDF (330KB) ( )   Taking the one-year seedlings of Ulmus crassifolia as subjects, which were introduced from America and cultivated in the field, the daily courses of its net production rate (Pn), transpiration rate (Tr) and water utilization efficiency (WUE) in leaves, and its influencing factors were studied under natural condition.The results showed that the curve of Pn diurnal variation was a single peak and its peak value appeared at 11:00;Tr daily courses of leaves from different parts were obviously one-peak curves.Tr peak values of both middle and bottom leaves appeared at 13:00 and peak value of top leaves came out at 15:00 and the descending order of Tr peak values was middle leaves, bottom leaves, top leaves;WUE daily courses of leaves from different parts all presented a one-peaked curve decreasing at one side, WUE of middle and bottom leaves lower than those of top leaves.By main component analysis, path coefficient analysis, or multiple return analysis, major physiological and ecological factors affecting Pn were temperature, Vpdl and light intensity;the influencing factors of Tr and WUE were temperature and Vpdl. Related Articles | Metrics

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Effects of Different Conditions of Tissue Culture on Vitrification of Plantlets from Broussonetia papyrifera SHI Qun, WEI Da-qi 2007, 36(03): 12-14.  Abstract ( )   PDF (164KB) ( )   MS medium was chosen as the basic medium and the main factors such as hormone, sucrose, agar, culture irradiation and temperature were investigated on vitrification of plantlets from Broussonetia papyrifera.The results showed that the coefficient of proliferation was higher and the rate of vitrification was lower when the plantlets from B. papyrifera were cultured in MS medium supplemented with 1.0 mg/L 6-BA, 0.3 mg/L NAA, 3.5% sucrose, 0.35% agar and under the conditions of culture illumination 2 000 lx and temperature 25℃. Related Articles | Metrics

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The Seed Morphology and Embryo Culture of Phalaenopsis Red Cultivars ZENG Bi-yu, ZHU Gen-fa, LIU Hai-tao, WANG Bi-qing 2007, 36(03): 15-18.  Abstract ( )   PDF (214KB) ( )   The morphological traits of seeds from 21 self-and cross-combinations in Phalaenopsis and their embryos culture were investigated.The average seed length and seed width were 0.292±0.025 mm and 0.073±0.003 mm, respectively.And the average embryo length and embryo width were 0.151±0.009 mm and 0.069±0.003 mm, respectively.The germination rate and the time required for germination were various because of different combinations.The average germination rate reached 72.4%.The mean time for PLBs and first leaf formation for all combinations were 22±3 and 78±6 days, respectively.The time for germination of cross-combinations was shorter than that of self-combinations and the germination rate was higher.There were no significant correlations among the time required for seed germination, germination rate, fruit size and seed size.But there was significantly linear negative correlation between the time required for seed germination and embryo size.It showed that the seeds with larger embryos would germinate earlier. Related Articles | Metrics

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Radioactive Breeding of Phalaenopsis spp.Using RAPD Method ZHANG Ning, SU Ming-hua, XU Su-xia, LIN Qing-hong 2007, 36(03): 19-22.  Abstract ( )   PDF (314KB) ( )   Using RAPD method, radiation-induced mutant seedlings of Phalaenopsis were studied.Five mutant seedlings of different formation and the control were amplified by PCR method, 13 arbitrary primers amplified 77 bands ranging from 200 bp to 3000 bp with an average of 5.92 bands per primer, among them 61 bands were polymerbhic and the percentage was 79.22%.The most bands were obtained from primer S0036.Compared the bands of mutant seedlings with those of control, there were differences among them and the results indicated that mutant seedlings were caused by gene mutation. Related Articles | Metrics

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Studies on the Optimum Condition of Trichoderma viride Persoon ex fries Fermentation to Produce Cellulase CHEN Jian-xuan, CHEN Lian 2007, 36(03): 23-26.  Abstract ( )   PDF (256KB) ( )   Solid-state fermentation process was carried out to convert brewer's grains to produce cellulase by Trichoderma viride Persoon ex fries.Such factors including initial moisture, fermentation time, initial pH value, the amounts of wheat bran and nitrogen source types were studied in this experiment.An optimum medium ingredient was obtained by primary test of single factor and orthogonal design experiment.The results were as follows.The best fermentation conditions were initial pH 5~6, fermentation temperature 28~30℃ and fermentation time 4d, and the best medium composition was wheat bran 30%, water contain 50% at least and (NH4)2SO4 2.5%. Related Articles | Metrics

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Defoliating insects of Loquat and the Efficacy Trials of Melanographia flexilineata in the Laboratory LUO Jia, XIE Chang, XIE Hui-feng 2007, 36(03): 27-30.  Abstract ( )   PDF (186KB) ( )   The investigation showed that among the 24 species of loquat leaf-eating pests, the most severe insect pests were Melanographia flexilineata and Phalera flavescens, followed by Thosea sinensis and Porthesia scintillans.The Melanographia flexilineata occurs 5 generations a year, and the first generation of larvae appeared in early May, which has the largest population.In this study, Abamectin, Acetamiprid and Fipronil were used to do indoor efficacy tests on Melanographia flexilineata.The results showed that 2% Abamectin diluted to 2 000 times, 3% Acetamiprid diluted to 1 000 times and 5% Fipronil diluted to 2 000 times had the best controlling effects. Related Articles | Metrics

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Control of Rhynchophorus ferrugineus Damaging to Phoenix canariensis in Xiamen LIU Wen-fang 2007, 36(03): 31-33.  Abstract ( )   PDF (230KB) ( )   Rhynchophorus ferrugineus is one of exotic insect pests of Palmae plants.It mainly distributes in Hainan, Guangxi, Guangdong, Taiwan, Fujian, Yunnan, Tibet (Motou) in China.The symptom of infection, morphological characters and living habits of Rhynchophorus ferrugineus were described.Based on the investigation of the damage of Rhynchophorus ferrugineus in Phoenix canariensis in Xiamen, some suggestions for control were proposed. Related Articles | Metrics

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Primary Investigation and Utilized Strategy of Beneficial Ladybugs in Garden Plants of Xiamen City CAI Hui, SUN Guo-kun, LIU Wen-fang, LIN Zhi-wei, XIE Yi-xuan 2007, 36(03): 34-36.  Abstract ( )   PDF (255KB) ( )   According to the investigation, there exits 22 genera and 33 species of ladybug in garden plants of Xiamen.The dominant species are Coccinella septempunctata, Rodolia pumila, R. rufopilosa, Micraspis disclor, Harmonia axyridis and Adalia bipunctata.The measures of protection and use of beneficial ladybugs were proposed in this paper. Related Articles | Metrics

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Establishment of Regeneration System of Pepper Cotyledon JING Guo-xing, LI Fu-qin, CHEN Yong-shuang, ZENG Fu-hua, LU Xiang-yang 2007, 36(03): 37-39,42.  Abstract ( )   PDF (188KB) ( )   Effects of plant regulators on adventitious buds differentiation and elongation of Bulgaria pointed pepper and chiyan No.6 were studied in the report.The results showed that the best combinations for the adventitious buds differentiation of Bulgaria pointed pepper and chiyan No.6 were LC 1.0 mg/L+IAA 0.1 mg/L and LC 1.0 mg/L+IAA 1.0 mg/L, respectively, with which the differentiation rates were all 100%, the rates of superior buds were 83.3% and 66.7%, respectively.Best combinations for elongation of adventitious buds of Bulgaria pointed pepper and chiyan No.6 were LC 0.1 mg/L+IAA 0.2 mg/L and LC 0.2 mg/L+IAA 0.6 mg/L, respectively, the elongation rates were 78.5% and 58.5%, respectively.The optimum rooting medium was 1/2 MS+IAA 1.0 mg/L+NAA 0.5 mg/L, and the average rooting rates were 100% and 85%, respectively. Related Articles | Metrics

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Studies on the Tissue Culture in Cymbidium Golden elf.‘Sundust’ DENG Ying, LING Xu-bai 2007, 36(03): 40-42.  Abstract ( )   PDF (154KB) ( )   The paper reported a propagation method of Cymbidium Golden elf.‘Sundust’ in Vitro culture of shoot-tips. The results showed that Protocorm-like-bodies (PLB) could be induced from shoot-tip explants in Hyponex without plant growth regulator. PLB multiplicated fast in Hyponex liquid medium supplemented with 6-BA 1.5 mg/L and developed into cluster PLB; mode of cultivation could obviously influence the multiplication rate of PLBs. PLBs could develop into plantlets without roots in MS or Hyponex medium. Hyponex medium supplemented with NAA 0.2mg/L and IBA 0.6mg/L was suitable for roots induction and growth. Related Articles | Metrics

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Effects of Edible Pigments on Dyeing of Cut Lily HUANG Xiao-kun, HUANG Xiao-dong 2007, 36(03): 43-45.  Abstract ( )   PDF (153KB) ( )   The effects of edible pigments on dyeing of cut lily were studied.The results showed that Fruit Green and Tartrazinne were more suitable for cut lily than Ponceau 4R, Amranth and Sun Set Yellow.The suitable dyeing density and time for different food colors were Fruit Green (15 g/L, 21 h), Tartrazinne (11.25 g/L, 12 h), Ponceau 4R (15 g/L, 10 h), Amranth (11.25 g/L, 20 h), Sun Set Yellow (15 g/L, 17 h).The vase life became shorter after dyeing but still had 7~9 days for ornament. Related Articles | Metrics

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Comparative Experiment of Four Preservatives HUANG Xiao-jun, PAN Ting-ting, LUO Guo-rong, CHEN Juan 2007, 36(03): 46-48,55.  Abstract ( )   PDF (786KB) ( )   The effects of simple preservatives containing sucrose, salt, alcohol, aspirin, vinegara and penicillin with different concentrations and various combination on three cut flowers Dianthus carryophylllus, Gerbera jamesonii, Rosa hybrida were studied by defermining the vase life, fresh weight and water balance of those cut flowers.It showed that preservative agent B (water 1 L+sucrose 10 g+salt 10 g+alcohol 10 ml) gave best result for preservation of cut flowers. Related Articles | Metrics

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Techniques of Cutting Propagation for Fokienia hodginsii ZHENG Rong, XIAO Xiang-xi, HUANG Xiu-mei, YANG Zong-wu, WEI Ying-jing, HUANG Sheng-ji 2007, 36(03): 49-52.  Abstract ( )   PDF (266KB) ( )   The cutting techniques of Fokienia hodginsii were studied in this paper.The results indicated that the optimum pruning time for mother-trees was from the end of February to the beginning of March, and the slip budding in June was suitable for cuttage.The mother-tree of 2 to 4 years was better for cutting.The root-inducing regulator could effectively promote rooting IBA with low density (100 mg/L) and short-time soaking (0.5 h) for the slip gave the best effects for rooting.The sandy soil was the better substrate for the growth of cutting.Based on the local climate, the cutting season of F. hodginsii should be in June and October in Fujian. Related Articles | Metrics

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Cultivation and Propagation of Euphorbia excelsa and Its Application to Landscape GUO Ling-yu, LI Wu-jin, ZHOU Qun, WANG Cheng-cong 2007, 36(03): 53-55.  Abstract ( )   PDF (741KB) ( )   This paper describes the botanical character of Euphorbia excelsa, presents the paths of cultivation and propagation, and its application in landscape.It expounds the potentiality and feasibility using as a landscape plant as well. Related Articles | Metrics

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The Key Protocols for the Standardization Production of Cymbidium ensifolium LIN Hong, CHEN Yin-feng 2007, 36(03): 56-58.  Abstract ( )   PDF (154KB) ( )   We recommened the key protocols for the standardization production of Cymbidium in this paper, which includes the requirements for establishment of Cymbidium garden, cultivation, routine management and pests control.The standardization is guidelines for the standardization production of Cymbidium. Related Articles | Metrics

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Studies on the Medicinal Pteridophytes in Tianmushan National Nature Reserve, Zhejiang Province YOU Zhi-mian, ZHANG Sen-yao, YAO Zhen-sheng 2007, 36(03): 59-63.  Abstract ( )   PDF (272KB) ( )   It introduced the medicinal pteridophytes in Tianmushan National Nature Reserve, Zhejiang Province.There exists 114 species of pteridophytes in this area, which belong to 57 genera of 32 families.The species distribution, and main chemistries were introduced, the measures of protection and the utilization of them were also discussed. Related Articles | Metrics

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Embryo Culture and Propagation of Atractylodes lancea ZHAO Yu-guo, WU Yan-you, SANG Xiao-hua 2007, 36(03): 64-64,66.  Abstract ( )   PDF (395KB) ( )   Related Articles | Metrics

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Rapid Propagation and Induction of Flower Buds of Phalaenopsis WANG Zai-hua, ZHU Gen-fa, Lü Fu-bing, YE Qing-sheng 2007, 36(03): 65-66.  Abstract ( )   PDF (298KB) ( )   Related Articles | Metrics

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Effects of NAA and 6-BA on Propagation with Protocorm-like-body of Phalaenopsis wilsonii CHEN Yin-feng, LIN Hong 2007, 36(03): 67-68.  Abstract ( )   PDF (124KB) ( )   Related Articles | Metrics

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Application and Prospect of Artificial Mutation Technology on Breeding for Resistance to the Diseases of Plants ZHANG Yan-ling, WU Li-rong, HE Hong 2007, 36(03): 69-73.  Abstract ( )   PDF (279KB) ( )   This paper introduces the main achievements of breeding for disease resistant of plants.It elaborates that artificial mutation connected with crossbreeding, gene transfer and in vitro selection could raise the breeding efficiency and enlarge the field of breeding for disease resistant.The application of artificial mutation technology is favorable to cultivate new disease-resistant varieties, and promote the development of agriculture. Related Articles | Metrics

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Advances in Biotechnological Research on Narcissus HUANG Shuang-long, LAI Zhong-xiong 2007, 36(03): 74-79.  Abstract ( )   PDF (382KB) ( )   Advances in biotechnological research on Narcissus, including in vitro plant regeneration, gene cloning, genetic transformation as well as their applications to micro propagation, virus free, transgenic improvement, germplasm and manufacture of secondary metabolite are reviewed, and the existing problems and intending prospects are also indicated. Related Articles | Metrics

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Brassinosteroids Signaling and Cell Expansion in Plant FAN Yu-qin 2007, 36(03): 80-84.  Abstract ( )   PDF (332KB) ( )   Upon perception of BRs, signals of BRI1/BAK1 receptor complex are transmitted through a phosphorylation cascade that includes both changes in gene expression and rapid growth induction responses that involve the V-ATPase.These pathways act separately to affect cell expansion processes.This review focuses on recent progresses in the studies of the gene expression regulation by BRs, BRs signaling and cell expansion in plant, and discusses the unsolved issues. Related Articles | Metrics

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Research Advances of Oncidium LIU Xiao-rong, WANG Bi-qing, ZHU Gen-fa 2007, 36(03): 85-90.  Abstract ( )   PDF (253KB) ( )   Based on the progress of the Oncidium at home and abroad in recent decade, this article summarizes its research results of tissue culture, cultivation and gene-engineering, also viewes its industrialization development in the future. Related Articles | Metrics