Abstract: The effects of 4-PU and 6-BA on formations of the calli and adventitious buds from epicotyl explants in peanut were studied in the experiment.in the MS medium contained 0.1 mg/L 4-PU and 6-BA 4 mg/L for 20 days, the formation rate of calli could reach 100%.0.1 mg/L 4-PU combined with 1-4mg/L 6-BA in MS medium, promoted the adventitious buds formation.The optimal medium for adventitious bud information and shoot elongation were MS+0.1 mg/L 4-PU+2 mg/L 6-BA and MS+0.1 mg/L 4-PU+4 mg/L BA, respectively.The percentage of adventitious bud formation, number and length of buds of peanut grown in the MS medium contained only 0.5 mg/L 4-PU were higher than those grown in the MS medium contained 0.5 mg/L 4-PU+6-BA.

Key words: 4-PU, 6-BA, peanut, calli, adventitious buds

摘要： 以‘汕油523’花生上胚轴为外植体,研究细胞分裂素4-PU和6-BA对愈伤组织诱导及不定芽发生的效应。结果表明,当MS培养基含有4-PU 0.1 mg/L和6-BA 4 mg/L培育20 d时,愈伤组织形成率为100%;0.1 mg/L 4-PU与适宜浓度的6-BA(1~4 mg/L)共同作用,促进上胚轴不定芽发生,不定芽形成与芽伸长的适宜培养基配方分别为MS+4-PU 0.1 mg/L+6-BA 2 mg/L和MS+4-PU 0.1 mg/L+6-BA 4 mg/L;0.5 mg/L 4-PU对不定芽出芽率、芽数与长度的作用均大于其与不同浓度6-BA混合作用的效果。

关键词: 4-PU, 6-BA, 花生, 愈伤组织, 不定芽