Abstract: Taking the leaves of loquat as materials, the improved method SDS was used for the RNA isolation. Base on the extracted total RNA, a AS gene conservative district sequence was cloned by the method of homology cloning and nested PCR. The results demonstrated that the conserved sequence was 854 bp in length, coded 284 amino acids and was named ejAS (NCBI number: JX173279.1). Though sequence analysis by Blast in NCBI, the results show that the ejAS gene had the highest homology and similarity (98%) to the Malus×domestica, and shared above 70% homology with the rerorted Aralia elata, Glycyrrhiza uralensis, Polygala tenuifolia, etc.

Key words: Loquat, AS gene, gene clone, homology

摘要： 以‘解放钟’枇杷（Eriobotrya japonica）叶片为材料，采用改良SDS法提取总RNA，通过同源克隆和巢氏PCR方法首次从枇杷叶片中分离得到AS基因的保守区序列。结果表明：该AS基因保守区长854 bp，编码284个氨基酸残基，命名为ejAS（GenBank注册号: JX173279.1）；经比对，与同为蔷薇科的嘎啦果的同源性最高，达98%，与辽东楤木、乌拉尔甘草、绿玉树等均有70%以上的相似性。

关键词: 枇杷, AS基因, 基因克隆, 同源性