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10 March 2013, Volume 42 Issue 01 Previous Issue    Next Issue

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Amyrin Synthase Gene Conservative District Sequence from Eriobotrya japonica ‘Jie Fang Zhong’ 2013, 42(01): 1-4.  Abstract ( )   PDF (372KB) ( )   Taking the leaves of loquat as materials, the improved method SDS was used for the RNA isolation. Base on the extracted total RNA, a AS gene conservative district sequence was cloned by the method of homology cloning and nested PCR. The results demonstrated that the conserved sequence was 854 bp in length, coded 284 amino acids and was named ejAS (NCBI number: JX173279.1). Though sequence analysis by Blast in NCBI, the results show that the ejAS gene had the highest homology and similarity (98%) to the Malus×domestica, and shared above 70% homology with the rerorted Aralia elata, Glycyrrhiza uralensis, Polygala tenuifolia, etc. References | Related Articles | Metrics

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Separation of Sperm Cells of Rhododendron simsii 2013, 42(01): 5-9.  Abstract ( )   PDF (350KB) ( )   The mature pollen of Rhododendron simsii is bicellular, containing one vegetative cell and one germ cell, with the sperm cells forming in pollen tube. Semi vitro technique was used in style pollinated to make pollen tube sprout from style. Then the pollen tube burst by osmotic shock method and released a pair of sperm cells which connected to vegetative nucleus. The sperm cells separated kept activities proved by FDA method. A fairly large number of separated sperm cells could be collected by micromanipulator. References | Related Articles | Metrics

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Relationship between Tourism and Vegetation Environment of Baihua Mountain in Beijing 2013, 42(01): 10-14.  Abstract ( )   PDF (233KB) ( )   According to field sampling and choosing index of vegetation landscape features, the relationship between tourism and vegetation environment in the nature reserve were analyzed. The results showed that the vegetation landscape features were less affected by natural factors, but each vegetation landscape features and natural factors had some relevance. In human factors, the width of tourist road and proportion of species companying with human had a significant negative correlation with ecological effect value, it showed that human activities had a greater impact on vegetation landscape. In conclusion, when ecological tourism was developed in the mountain nature reserve, ecotourism planning and management must be strengthened, and the impact of human factors on the vegetation landscape should be reduced. References | Related Articles | Metrics

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Response of Native Tree Species Adapted to Air Pollution in Urban Green Land 2013, 42(01): 15-22.  Abstract ( )   PDF (339KB) ( )   In order to check the adaptability of 14 kinds native tree species of South China adapted to green land with different air pollution stress, the species were planted in 4 types green land and taken into physiology and ecology research. 4 types green land included botanical garden, park with more total suspended particulate than that in botanical garden, green land in industrial estate with more nitrogen oxides and total suspended particulate, green land of busy road, which is the most polluted land of the city. The result suggested that: the ways and the ability adapted to different types green land were not the same among the native tree species. With the pollution stress increasing, the correlation of the Pn with Gs in most native tree species decreased, and the change level of correlation coefficient was also different. Among them, 9 kinds native tree species including Michelia maudiae, Cinnamomum camphora, Elaeocarpus sylvestris, Gardenia jasminoides, Cyclobalanopsis hui, Psychotria asiatica, Erythrophleum fordii, Cryptocarya concinna and Machilus chinensis were sensitive to all the pollution stress, while Syzygium hancei and Helicia cochinchinensis were not. Photinia serrulata, Desmos chinensis and Liquidambar formosana were sensitive to some types pollution stress but not to the others. References | Related Articles | Metrics

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Effect of Nitrogen Fertilizer on Recovery Growth of Zoysia japonica Trample Stressed 2013, 42(01): 23-26.  Abstract ( )   PDF (215KB) ( )   Based on the data collected from field tests and lab experiments, the effect of nitrogen fertilizer on recovery growth of Zoysia japonica trample stressed was studied. The results showed that nitrogen fertilizer was beneficial to the recovery growth of Z. japonica trample stressed. Applying nitrogenous fertilizer with the condition of 8 g/m2 could increase the density, plant height, aboveground biomass; and total chlorophyll content was increased significantly than the control. References | Related Articles | Metrics

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Research on Techniques of Tissue Culture and Rapid Propagation of Wild Cymbidium lowianum 2013, 42(01): 27-30.  Abstract ( )   PDF (233KB) ( )   With the in vitro germination plantlet of wild Cymbidium lowianum in Yunnan as test material, a series of tissue culture and rapid propagation system research including induced proliferation and rooting for its multiple shoots was carried out. The results showed that the suitable multiplication medium was 1/2MS + 6-BA 2.0 mg/L + KT 0.5 mg/L + NAA 0.2 mg/L, with the proliferation rate of 300%, and the rooting medium was 1/2MS + NAA 1.5 mg/L + 6-BA 0.3 mg/L, then the roots healthy and strong. References | Related Articles | Metrics

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Preliminary Study on Cutting Propagation of Camellia chekiangoleosa Shoots 2013, 42(01): 31-34.  Abstract ( )   PDF (499KB) ( )   Through orthogonal design in the fields, the effects of ABT1# rooting powder with different concentrations and different treatment time on cuttings survival of Camellia chekiangoleosa were studied. The results showed that: the ABT1# rooting powder treatment could significantly improve the survival rate of C. chekiangoleosa shoots cuttings, and ABT1# rooting powder was the key factors that influenced the cuttings survival. The combination with the highest survival rate in this experiment was A2B2C1 that 100 mg/L ABT1# on softwood shoots spike top section of C. chekiangoleosa for 2 hours. C. chekiangoleosa belongs to the type of rooting from callus. References | Related Articles | Metrics

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Anatomical Observation on the Rooting Process of Camellia oleifera Cuttings 2013, 42(01): 35-39.  Abstract ( )   PDF (715KB) ( )   The growth course of adventitious roots from Camellia oleifera cuttings was observed with anatomical method. The results showed as follows: there was not incubative root primordium in one-year-old shoot of C. oleifera, and there were cyclical and continuous arranging sclerenchymatous cells in periderm. These structure features might be related to the longer rootage duration of cuttings from C. oleifera. The adventitious roots developed from the induced root primodium, which originated from the cells of vascular cambium, phloem and callus. The adventitious root of C. oleifera belonged to initial type and mixed rooting type of induced root. Just only a very few adventitious roots, which had no direct relationship with callus, developed from callus. References | Related Articles | Metrics

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Effects of 60Co-γRadiation on Grafting Survival Rate and Growth of Longan Scion 2013, 42(01): 40-42.  Abstract ( )   PDF (207KB) ( )   The effects of 60Co-γ radiation with different doses on grafting survival rate and growth of Shixia longan scion were discussed and observed in this experiment. The results showed that the grafting survival rate and growth of longan scion were influenced by 60Co-γradiation in various degrees. After treated by 60Co-γ, the survival rate was lower than the contrast, further, with the increasing of the treatment dose, the survival rate descended, and the growth of new shoots were also inhibited to a certain degree. References | Related Articles | Metrics

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Effect of Light and Temperature on Seed Germination of Clematis ranunculoides 2013, 42(01): 43-45.  Abstract ( )   PDF (320KB) ( )   In order to study the effect of light and temperature on seed germination of Clematis ranunculoides and its basic biological characteristics, its wild plant seeds were collected, moreover, the seed’s appearance was observed, and the thousand-seed weight was detected. The laboratory test method was used to determine the germination rate and potentiality under different conditions of light and temperature. The results showed that the thousand-seed weight of C. ranunculoides was 1.6624±0.0006 g. The optimal temperature for the seed germination was 20~25 ℃, and at this temperature, the seed begin to germinate in 8 days after seeding, and the germination procedure finished in 3~4 weeks, with the germination rate of 90.33%. Both higher and lower temperature could cause the decrease of germination rate, while light and dark did not influence significantly on the germination rate of C. ranunculoides seed. References | Related Articles | Metrics

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The Effect of Different LED Light on the Growth of Anoectochilus roxburghii 2013, 42(01): 46-48.  Abstract ( )   PDF (432KB) ( )   Anoectochilus roxburghii was used for the study of the effect of different light of light-emitting diode (red, yellow, blue) on its growth index, chlorophyll and stoma. The results showed that red and yellow light were better for the plant height which increased by 18.74%, 21.47%, respectively. But the content of chlorophyll a and chlorophyll b under red and yellow light treat are lower than CK and the plants are weaker. A. roxburghii grew well under blue light, leaf area, and the content of chlorophyll increased a lot than those of CK. The frequency, length and width of each treatment plant stoma have a certain growth. References | Related Articles | Metrics

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Dynamics of Polyphenol Oxidase ( PPO) Activity and Total Phenol Content of Actinidia arguta var. purpurea Shoots 2013, 42(01): 49-52.  Abstract ( )   PDF (235KB) ( )   Using the twigs and petioles of Actinidia arguta var. purpurea as test materials, the polyphenol oxidase (PPO), total phenol content, and their change pattern were analyzed. The results showed that, the suitable pH value for determining A. arguta var. purpurea PPO activity was 7.0, the optimal substrate was oxophenic acid, with the concent of 0.16 mol/L, and the optimum temperature was 25 ℃. The PPO activity of A. arguta var. purpurea at growth phase was higher than at dormancy stage. The total phenol content of A. arguta var. purpurea was the minimum in April, May and September. References | Related Articles | Metrics

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Contributions to the Taxonomy of the Genus Phyllostachys (Gramineae: Bambusoideae) 2013, 42(01): 53-64.  Abstract ( )   PDF (1240KB) ( )   Two species and five forms of Phyllostachys Sieb. et Zucc. (Bambusoideae), Ph. longiciliata G. H. Lai, Ph. zhejiangensis G. H. Lai, Ph. angusta McClure f. flavosulcata G. H. Lai, Ph. aurea Carr. ex A. et C. Riv. f. koi G. H. Lai, Ph. edulis (Carr.) H. de Lehaie f. abbreviata G. H. Lai, Ph. hirtivagina G. H. Lai f. flavovittata G. H. Lai and Ph. nidularia Munro f. basipilis G. H. Lai, are described as new to science, and the photos of the living plants are provided. Three new combinations, Ph. edulis (Carr.) H. de Lehaie f. pachyloen (G. Y. Yang et al.) Y. L. Ding, Ph. edulis (Carr.) H. de Lehaie f. anjiensis (P. X. Zhang) G. H. Lai and Ph. violascens (Carr.) A. et C. Riv. f. viridisulcata (P. X. Zhang et W. X. Huang) G. H. Lai, are proposed. The typification of Ph. aureosulcata McClure f. flavostriata S. J. Zhao, Ph. bambusoides Sieb. et Zucc. f. duihuazhu C. J. Wu, Ph. bambusoides Sieb. et Zucc. f. mixta Z. P. Wang et N. X. Ma and Ph. edulis (Carr.) H. de Lehaie f. holochrysa (Muroi et K. Kasahara) Ohrnberger is discussed, and a lectotype and three neotypes are newly designated here. References | Related Articles | Metrics

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New Distribution Record of Angiosperm in Fujian (Ⅴ) 2013, 42(01): 65-67.  Abstract ( )   PDF (312KB) ( )   According to the investigation on the medicinal plant resources in Fujian Province, some newly recorded taxa of angiosperm were found, i.e. one family Triuridaceae, two genera Sciaphila and Eleutherine, and four species, Erechtites valerianaefolia, Sciaphila megastyla, Eleutherine plicata, and Burmannia itoana. The voucher specimens were deposited in the Herbarium of FJTCM. References | Related Articles | Metrics

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The Composition of Oil Seed Plants in Longdi Nature Reserve of Southwest Hunan and their Resource Value Analysis 2013, 42(01): 68-72.  Abstract ( )   PDF (249KB) ( )   In order to provide the basis for utilizing plant resources, the compositions of oil seed plants in Longdi Nature Reserve in Southwest Hunan and their resources characteristics were analyzed. The result showed that (1) there were 477 species of oil seed plants, belong to 107 families and 281 genus, and among them, there were 382 species of oil plants and 131 species of essential oil plants, and 11 families including Lauraceae( 7 genus 27 species), Rutaceae( 6 genus 21 species), Labiatae( 11 genus 17 species) were the dominant families. The growth forms were dominated by woody plants, accounting for 73.38%. (2) The main oil-containing part was seed coat in 71.47% oil plants, and there were 211 species with the oil content above 20.0%, 43 species above 50.0%; and 11 species in 7 families with the oil fatty acid content beyond 70.0%, 20 species in 12 families with the linoleic acids content beyond 70.0%. (3) In oil plants, there were 57 species containing essential oil above 1.0%, 17 species above 4.0%. In conclusion, the oil plants resources in the reserve, especially oil-rich plants are abundant; the reserve is the place possessing enrichment oil plants, with development potential. At the same time, some recommendations for the sustainable management and utilization of oil plants were put forward. References | Related Articles | Metrics

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The Resources and Protection of Psammophilous Vegetation on Coasts in Xiamen Guanyinshan, Fujian Province 2013, 42(01): 73-76.  Abstract ( )   PDF (264KB) ( )   There are abundant plant resources of psammophilous vegetation on the coast of Xiamen Guanyinshan, including 135 species of vascular plants belonging to 42 families and 113 genera. Compared with the historical investigation data twenty-three years ago, the pioneer plants like Spinifex littoreus, Sporobolus virginicus and others are nearly extinct. In order to change the secondary vegetation status on the coast, the remnant forest and shrub should be protected strengtheningly. And the coastal protection forest system should be constructed on the basis of coastal psammophilous vegetation, thus to improve the environment condition and effectiveness against natural disasters. References | Related Articles | Metrics

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Studies on Introduction and Selection of Vertical Greening Plants in Quanzhou 2013, 42(01): 77-81.  Abstract ( )   PDF (287KB) ( )   Thirty kinds of climbing plants were introduced from surrounding areas of Quanzhou, and their survival rate, growth, major phenological period and stress resistance were tested in this paper. 29 of the climbing species that were suitable to be vertical greening plants in Quanzhou were selected as a result by the percentage evaluation method according to experiments. And 11 of the suitable climbing species were studied to be the principal varieties for popularization and application, which met the necessary that landscape diversity and plant species diversity in the vertical greening of Quanzhou. References | Related Articles | Metrics

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The Advantage, Problem and Development Countermeasure of the Industry of Anoectochilus roxburghii in Nanjing County, Fujian Province 2013, 42(01): 82-85.  Abstract ( )   PDF (260KB) ( )   According to the in-depth investigation on the industry of Anoectochilus roxburghii in Nanjing County, Fujian Province, the development advantages, problems and following development countermeasures are analyzed in this paper. The results show that there are inherent advantages for developing the industry of Anoectochilus roxburghii in Nanjing County, and the industry has achieved a certain scale and has produced a significant level of economic and social benefits. However, there also exist problems such as low industry cluster degree, lack of standardization strategies and technology input, and insufficient extension of industrial chain. The author hold that it is necessary to cultivate industrial cluster and pursue the path of joint development, implement regional brand strategy and promote standardization process, exploit product and expand industrial chain, and increase investment in science and technology and upgrade the overall industry. References | Related Articles | Metrics

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Biological Characteristics of Griffonia simplicifolia and its Chemical Composition 2013, 42(01): 86-90.  Abstract ( )   PDF (277KB) ( )   The modern phytochemistry study found that 5-hydroxytryptophan was the main chemical composition of Griffonia simplicifolia, and modern pharmacology researches showed that it had some pharmacological activities including depression, inhibition of obesity, antioxidation and so on. This article systematically introduced the biological characteristics of Griffonia simplicifolia and some correlative researches about its chemical composition and pharmacological efficacy, which provides a theoretical basis for its introduction, domestication and further development. References | Related Articles | Metrics