| [1] Kranz E, Bautor J, Lorz H. In vitro fertilization of single isolated，gametes of maize mediated by electrofusion[J]. Sexual Plant Reproduction, 1991,4: 12-16.[2] Kranz E, Lorz H. In vitro fertilization with isolated, single gametes results in zygotic embryogenesis and fertile maize plants[J]. Plant Cell, 1993, 5: 739-746.[3] Uchiumi T, Uemura I, Okamota T. Establishment of an in vitro fertilization system in rice[J]. Planta 2007,226: 581-589[4] Xu H L, Swoboda I, Bhalla P L et al. Male gametic cell-specific expression of H2A and H3 histone genes[J]. Plant Molecular Biology, 1991, 39: 607-614[5] 苟小平,徐莺,唐琳,等. 水稻精细胞cDNA文库的构建及分析[J]. 植物学报, 2001,43(10): 1 093-1 096.[6] Xu H P, Weterings K, Vriezen W et al. Isolation and characterization of male-germ-cell transcripts in Nicotiana tabacum[J]. Sexual Plant Reproduction, 2002,14: 339-346[7] Singh M B, Xu H L, Bhalla P L. Developmental expression of polyubiquitin genes and distribution of ubiquitinated proteins in generative and sperm cells[J]. Sexual Plant Reproduction, 2002,14: 325-329[8] Bai Y, Xu Y, Tang L et al. Molecular clonging and preliminary study of a gene differentially expressed in rice sperm cell[J]. Acta Botanic Sinica, 2003,45(3): 346-351.[9] Engel M L, Chaboud A, Dumas C et al. Sperm cells of Zea mays have a complex complent of mRNAs[J]. Plant Journal, 2003,34: 697-707.[10] Xin H P, Peng X B, Ning J et al. Expressed sequence-tag analysis of tobacco sperm cells reveals a unique transcriptional profile and selective persistence of paternal transcripts after fertilization[J]. Sexual Plant Reproduction, 2011,24: 37-46[11] Cass D D. An ultrastructural and Nomarsiki-interference study of the sperms of barlely[J]. Can J Bot, 1973,51: 601-605.[12] Russell S D. Isolation of sperm cells from the pollen Plumbago zeylanica[J]. Plant Physiology, 1986,81: 317-319.[13] Dupuis I,Roeckel P, Matthys-RochonE. Procedure to isolate viable sperm cells from corn Zea mays L. pollen grain[J]. Plant Physiology, 1987,85: 876-878.[14] Nielsen J E, Olesen P, Wilms H J. Isolation of sperm cells from the trinucleate pollen of sugar beet (Beta vulgaris)[M]// Wilms H J, Keijizer C J. Plants Sperm Cells as Tools for Biotechnology, Wageningen: Pudoc, 1988: 111-112.[15] Cass D D, Hough R B, Knox C A et al. Isolation of sperms from pollen of corn and oil seed rape[J]. Plant Physiology, 1986,80(supp1): 130.[16] 莫永胜,杨弘远. 紫菜苔精细胞的大量分离和生活力的保存[J]. 植物学报, 1991,33(9): 649-657.[17] 宋玉燕,包晗,陈美灵,等. 胡萝卜精、卵细胞、助细胞和中央细胞的分离[J]. 植物生理学报, 2012,48(1): 90-94.[18] Read S M, Clarke A E, Bacic A et al. Requirements for division of generative nucleus in cultured pollen tubes of Nicotiana.[J]. Protoplasma, 1993,174: 101-115[19] Shivanna K R, Xu H, Taylor P et al. Isolation of sperms from the pollen tubes of flowering plants during fertilization[J]. Plant Physiology 1988,87: 647-650[20] 莫永胜,杨弘远. 几种具二细胞型花粉植物精细胞的分离和融合[J]. 植物学报, 1992,34(9): 688-697.[21] Tian H Q, Russell SD. The fusion of sperm cells and the function of male germ unit (MGU) of tobacco (Nicotiana tabacum L.)[J]. Sexual Plant Reproduction, 1998,11: 171-176 |
