Development of SSR Molecular Markers Based on Transcriptome Sequencing of Eriobotrya japonica

doi:10.3969/j.issn.1009-7791.2015.04.002

›› 2015, Vol. 44 ›› Issue (04): 274-278.DOI: 10.3969/j.issn.1009-7791.2015.04.002

• Plant physiology, biochemistry and molecular biology • Previous Articles Next Articles

Development of SSR Molecular Markers Based on Transcriptome Sequencing of Eriobotrya japonica

ZHENG Ting-ting,WEI Wei-lin,YANG Xiang-hui,LIN Shun-quan

(1.College of Horticulture, South China Agricultural University, Guangzhou 510642, Guangdong China; 2.Shangrao City Forest Resources Monitoring Center, Shangrao 334000, Jiangxi China)

Received:2015-10-20 Revised:2016-01-15 Online:2015-12-10 Published:2015-12-10
Contact: YANG Xiang-hui; LIN Shun-quan

基于枇杷转录组序列的SSR分子标记引物开发

郑婷婷,魏伟淋,杨向晖,林顺权

(1.华南农业大学园艺学院，广东广州 510642；2.上饶市森林资源监测中心，江西上饶 334000)

通讯作者: 杨向晖;林顺权
作者简介:郑婷婷，硕士，从事果树育种及生物技术研究。
基金资助:
广州市科技计划项目(201504010028)

Abstract

Abstract: In order to obtain more loquat SSR primers, the SSR locis were analyzed using microsatellite locus scan tool (SSRIT) to analyze 11 798 unigenes (>1 kb) based on the transcriptome sequencing data from flesh of loquat, and we screened out 4438 SSR locis which distributed in 3515 unigenes, accounting for 6.77% of the unigenes. The di-nucleotide and tri-nucleotide repeats were the most abundant repeat types, accounting for 68.27% of the total number of SSRs. And the tetra-nucleotide, penta-nucleotide and hexa-nucleotide repeats were the minor repeat types, accounting for only 1.42%. There were 7911 primers obtained through Primers3 software, which had an important application value to analyze genetic diversity, molecular assistant breeding and establishment of breeding population of loquat.

Key words: Eriobotrya japonica, transcriptome, SSR

摘要： 为获得更多的枇杷SSR引物，对枇杷转录组测序得到的1 kb以上的11 798条Unigenes进行SSR位点搜索。结果在3515 条Unigenes（6.77%）中共获得4438个SSR位点，其中主要重复类型为双碱基重复和三碱基重复，二者占SSR总数的68.27%，而四、五、六碱基重复类型较少，仅占1.42%。对选出的SSR标记采用Primer3进行引物设计，得到7911 对SSR位点特异引物，可用于枇杷遗传多样性分析、分子标记辅助育种、育种群体的建立等研究。

关键词: 枇杷, 转录组, SSR

CLC Number:

S667.3

ZHENG Ting-ting,WEI Wei-lin,YANG Xiang-hui,LIN Shun-quan. Development of SSR Molecular Markers Based on Transcriptome Sequencing of Eriobotrya japonica[J]. , 2015, 44(04): 274-278.

郑婷婷,魏伟淋,杨向晖,林顺权. 基于枇杷转录组序列的SSR分子标记引物开发[J]. , 2015, 44(04): 274-278.

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Development of SSR Molecular Markers Based on Transcriptome Sequencing of Eriobotrya japonica

基于枇杷转录组序列的SSR分子标记引物开发

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