Subtropical Plant Science ›› 2016, Vol. 45 ›› Issue (01): 42-47.DOI: 10.3969/j.issn.1009-7791.2016.01.009

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Characteristics and Expression of Dormancy-Associated MADS-Box PpMADS13 in Different Chilling Requirement Pear Cultivars

LI Liang,LIU Hang,ZHANG Gui-chi,WU Shao-hua,LI Yong-yu   

  1. (College of Horticulture, Fujian Agriculture and Forestry University; Institute of Natural Products of Horticultural Plants, Fuzhou 350002, Fujian China)
  • Received:2015-12-08 Revised:2016-04-17 Online:2016-03-10 Published:2016-03-10
  • Contact: WU Shao-hua; LI Yong-yu

不同低温需求量砂梨休眠相关基因PpMADS13的特征及表达模式

李亮,刘杭,张桂池,吴少华,李永裕   

  1. (福建农林大学园艺学院,园艺植物天然产物研究所,福建 福州 350002)
  • 通讯作者: 吴少华;李永裕
  • 作者简介:李亮,硕士研究生,从事果树分子生物学研究。
  • 基金资助:

    国家自然科学基金(31501694);高等学校博士学科点专项科研基金(20113515110011)

Abstract: The buds from ‘Huanghua’ and ‘Mixue’, two cultivars of Pyrus pyrifolia, were used as the experiment materials to explore the characteristics and expression of dormancy-associated MADS-box (DAM) genes PpMADS13-1 and PpMADS13-3 in different chilling requirement pear cultivars from Fujian. PpMADS13 genes were cloned and used the QRT-PCR to analysis their expression changes during the dormancy period. The results showed that PpMADS13-1 was conservative in low chilling requirement pears. The conservatism of PpMADS13-3 was not so high in different chilling requirement pears. The PpMADS13-1hh and PpMADS13-3hh in ‘Huanghua’ were gradual down-regulation with dormancy release. The PpMADS13-1mx and PpMADS13-3mx in ‘Mixue’ presented a trend of ‘M’ type during the dormancy. The result indicated that both PpMADS13-1 and PpMADS13-3 work in dormancy release, but their regulation model were different between two pear cultivars.

Key words: Pyrus pyrifolia, dormancy-associated MADS-box, expression

摘要: 为探究福建主栽的不同低温需求量砂梨Pyrus pyrifolia品种中休眠相关基因PpMADS13-1和PpMADS13-3序列及表达特征,以‘黄花’、‘蜜雪’两品种休眠时期的花芽为材料,克隆PpMADS13-1和PpMADS13-3基因,利用荧光定量PCR技术分析基因在休眠时期的表达变化情况。结果显示,PpMADS13-1序列在砂梨两品种间较保守,而PpMADS13-3序列在两品种间保守性较差;‘黄花’梨PpMADS13-1hh和PpMADS13-3hh在休眠期间随着休眠的解除下调表达,‘蜜雪’梨PpMADS13-1mx和PpMADS13-3mx在休眠期间呈现“M”型表达趋势。PpMADS13-1和PpMADS13-3均对砂梨的花芽休眠解除起到调控作用,在不同低温需求量品种间PpMADS13-1和PpMADS13-3的休眠调控模式有所不同。

关键词: 砂梨, 休眠相关MADS-box, 表达

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