Abstract: A UPLC method for the determination of acetoside in Macfadyena unguis-cati was established. Single factor and orthogonal experiments for acetoside extraction from M. unguis-cati were designed. Macroporous resins were screened for the enrichment of acetoside and the elution procession was determinated. The UPLC conditions: HSST3 column (2.1 × 100 mm, 1.8 μm), mobile phase 0.1% HCOOH(A)–CH3CN(B), DAD detector at 310 nm, flow rate 0.3 mL·min-1, column temperature 25 ℃, elution process: 90%A-5 min, 75%A-7.5 min, 30%A-9 min, 90%A. The results showed that the calibration curves of acetoside was in a good linearity over the range of 0.25—100 ng under the experiment condition and the regression equation was Y=89.002X-4.8275 (R=0.9999). The best extraction solvent was 70% ethanol according single factor experiment and the optimum extraction conditions were 70% ethanol, solid-liquid ratio of 1∶10 and 12 h extraction time through orthogonal experiments. The most suitable macroporous resin for acetoside enrichment was HP-20 through static and dynamic adsorption and desorption experiments. The enrichment process by HP-20 resin was gathering 4 BV of the 40% ethanol elute after 4 BV of elution by 15% ethanol.

Key words: Macfadyena unguis-cati, acteoside, extraction, macroporous resin

摘要： 建立UPLC测定猫爪藤Macfadyena unguis-cati阿克替苷含量的方法，采用单因素实验和正交试验优化阿克替苷提取工艺，通过静态、动态吸附与解吸实验筛选适合富集阿克替苷的大孔树脂，并对富集工艺进行研究。UPLC条件：色谱柱HSST3柱(2.1 × 100 mm，1.8 μm)；流动相0.1%甲酸(A)-乙腈(B)；梯度90%A 5 min，75%A 7.5 min，30%A 9 min，90%A；检测波长310 nm；柱温25 ℃；流速0.3 mL·min-1；保留时间5.21 min。在UPLC条件下阿克替苷在0.25～100 ng进样范围内峰面积与进样量呈良好的线性关系，回归方程为 Y= 89.002X - 4.8275，R=0.9999。单因素实验显示，70%乙醇为最佳提取溶剂。正交试验确定最佳提取条件为70%乙醇，料液比1∶10，提取时间12 h。静态、动态吸附和解吸实验确定HP-20大孔树脂为最佳富集材料。富集工艺为4倍体积15%乙醇洗脱除杂后收集4倍体积40%乙醇洗脱的部分。

关键词: 猫爪藤, 阿克替苷, 提取, 大孔树脂