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30 June 2017, Volume 46 Issue 02 Previous Issue    Next Issue
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Plant physiology, biochemistry and molecular biology
Plant ecology, resources and taxonomy
Plant cultivation
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Plant physiology, biochemistry and molecular biology

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Adhesion and Detection of Arabidopsis Protoplasts Based on Surface Protonated Poly(dopamine) Modified Electrode ZHOU Tie-an,ZHANG Na,SU Zhao-hong,SHEN Da-zhong,CHEN Zong-xing,DENG Jun,XIE Jie,ZOU Jian-feng 2017, 46(02): 101-107.  DOI: 10.3969/j.issn.1009-7791.2017.02.001 Abstract ( )   PDF (1245KB) ( )   In this study, dopamine was polymerized onto the surface of optically transparent ITO (or Au) electrode by auto-oxidation method. The formed polydopamine film was then protonated in pH 3 buffer solution to become positively charged which facilitates electrostatic interaction with negatively charged protoplasts. The effectiveness of the protonated polydopamine films to the adhesion of Arabidopsis thaliana protoplasts was confirmed by cyclic voltammetry and electrochemical impedance spectroscopy. In the tested range of 1000—30 000 protoplasts, the charge-transfer resistance (Rct) of the protonated polydopamine films increased with the protoplast number Ncells, and the 1/Rct displayed a linear relationship with 1/Ncells. Moreover, the dynamic adhesion test results using quartz crystal microbalance further proved that the protonated polydopamine films had good adhesion to the protoplasts. This study provides an effective method for the immobilization and sensing of protoplasts, which is an important step toward the goal of study of plant structure, function and behavior at the cellular level and the study of plant life diversity. References | Related Articles | Metrics

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Extraction and Enrichment of Acteoside from Macfadyena unguis-cati (Bignoniaceae) LI Xu-dan,LIU Hong-zhou,CAO Hui-cong,ZHENG Zhi-zhong,LIU Shao-song 2017, 46(02): 108-112.  DOI: 10.3969/j.issn.1009-7791.2017.02.002 Abstract ( )   PDF (1104KB) ( )   A UPLC method for the determination of acetoside in Macfadyena unguis-cati was established. Single factor and orthogonal experiments for acetoside extraction from M. unguis-cati were designed. Macroporous resins were screened for the enrichment of acetoside and the elution procession was determinated. The UPLC conditions: HSST3 column (2.1 × 100 mm, 1.8 μm), mobile phase 0.1% HCOOH(A)–CH3CN(B), DAD detector at 310 nm, flow rate 0.3 mL·min-1, column temperature 25 ℃, elution process: 90%A-5 min, 75%A-7.5 min, 30%A-9 min, 90%A. The results showed that the calibration curves of acetoside was in a good linearity over the range of 0.25—100 ng under the experiment condition and the regression equation was Y=89.002X-4.8275 (R=0.9999). The best extraction solvent was 70% ethanol according single factor experiment and the optimum extraction conditions were 70% ethanol, solid-liquid ratio of 1∶10 and 12 h extraction time through orthogonal experiments. The most suitable macroporous resin for acetoside enrichment was HP-20 through static and dynamic adsorption and desorption experiments. The enrichment process by HP-20 resin was gathering 4 BV of the 40% ethanol elute after 4 BV of elution by 15% ethanol. References | Related Articles | Metrics

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Antitumor Activity of Extracts from Voacanga africana Seeds ZHOU Jing,LIU Jia-jia,WU Yi-qiang,YUAN Guang-ming 2017, 46(02): 113-116.  DOI: 10.3969/j.issn.1009-7791.2017.02.003 Abstract ( )   PDF (876KB) ( )   With different polarity organic solvents (n-hexane, dichloromethane, ethyl acetate, acetone and ethanol), extraction yield of Voacanga africana seeds by Soxhlet extraction were determinated. The inhibitory activity of extracts against human tumor cell proliferation was tested using Hela Cells and SMMC-7721 Cells as the experimental object and the MTT method as judge standards. The results indicated that V. africana seed extracts of acetone showed excellent inhibitory activity on the proliferation of SMMC-7721 cells and a general inhibition towards Hela cancer cells. And the n-hexane extracts also had relatively significant cytotoxic activity on Hela cancer cells, while the activity towards SMMC-7721 cells was not significant; other organic phase extracts possessed almost no inhibition activity. References | Related Articles | Metrics

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Effect of Water Stress on Physiological Characteristic of Camellia fluviatilis QI Yan,SU Dan-Ping,LI Wei,LIU Chao-yu,CUI Da-fang 2017, 46(02): 117-121.  DOI: 10.3969/j.issn.1009-7791.2017.02.004 Abstract ( )   PDF (1554KB) ( )   The physiological indexes of Camellia fluviatilis under waterlogging stress were studied. The results showed that with the extension of flooding time, the chlorophyll contents of both waterlogging treatments were lower than those of control group. Under waterlogging stress, SOD activity, soluble protein and MDA contents of the waterlogging treatments tended to rise first, then fall and rise again as the flooding time extending. The content of soluble sugar was falling first and then rising. All physiological indexes indicated C. fluviatilis was adapted to waterlogging stress in a certain degree. References | Related Articles | Metrics

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Techniques of Reducing Tissue Culture Browning of Lilium lancifolium LIANG Chun-hui,YAN Xiao-dong,HUANG Min,LI Rong-xi,CHEN Li-fen,LI Xiu-ping 2017, 46(02): 122-125.  DOI: 10.3969/j.issn.1009-7791.2017.02.005 Abstract ( )   PDF (861KB) ( )   Lilium lancifolium bulb scales as explants, 1/2MS+ 0.8 mg·L-1 6-BA+ 0.2 mg·L-1 NAA as the basic medium, the effects of different time on disinfection and the impact on browning with polyvinylpyrrolidone (PVP), ascorbic acid(V?), activated carbon (AC), light and dark treatments were studied. The results showed that there were two disinfection methods having the best effect, one was 75% ethanol for 30 s, followed by 0.1% HgCl2 for 10 min, the other was 2% NaClO for 6 min, the contamination rates of both were 33.33%, the survival rate and callus induction rate of both were 66.67%. Browning could be reduced with the medium involved in polyvinylpyrrolidone (PVP), V?, activated carbon, and in the dark conditions. The best effect of browning was 4.5 mg·L-1 PVP, the browning rate was 30%, callus induction rate was up to 70%. References | Related Articles | Metrics

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Leaf Traits of Different Litchi chinensis Cultivars HUANG Xiao-feng,WEI Yang-lian,YU Jin-chang,TIAN Hai-juan,YUAN Zhi-yong 2017, 46(02): 126-130.  DOI: 10.3969/j.issn.1009-7791.2017.02.006 Abstract ( )   PDF (855KB) ( )   The leaf traits, such as specific leaf area, leaf dry matter content and leaf SPAD value of fourteen Litchi chinensis cultivars were analyzed in this study. The results showed that specific leaf area of three L. chinensis cultivars with apiculate peel were greater than that of two cultivars with flat peel. Among nine cultivars with ridge peel, specific leaf area of five cultivars was greater than that of ‘Heiye’, a cultivar with flat peel, and specific leaf area of four other cultivars was smaller than that of ‘Heiye’. Of all the Litchi chinensis cultivars, speci?c leaf area of thirteen cultivars had positive correlation with leaf dry matter content except ‘Heiye’. The correlation between speci?c leaf area and leaf SPAD value was positive for ‘Shuijingqiu’ and ‘Dadingxiang’, but negative for the other twelve cultivars. References | Related Articles | Metrics

Plant ecology, resources and taxonomy

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Seedling Growth and the Environmental Factors of the Non-native Mangrove Species, Sonneratia caseolaris, in Shenzhen Bay CHEN Ye-bin,GUO Xu-dong,LI Kang-ming,CHEN Lu-zhen 2017, 46(02): 131-136.  DOI: 10.3969/j.issn.1009-7791.2017.02.007 Abstract ( )   PDF (1151KB) ( )   This study aimed to discover the growth, photosynthetic traits of Sonneratia caseolaris seedlings to different environment in natural mangroves, which were four kinds of plots under dense canopy of Kandelia obovata forests and S. caseolaris forests, forest gaps, and mudflats. The results showed that the seedlings growing in mudflats had the largest total biomass and leaf biomass partitioning, attributing to their high photosynthetic rates, water use efficiency and photosynthetic nitrogen use efficiency. Light intensity was the key factor among the all environmental factors that contributing to the fast growing of S. caseolaris seedlings, which led to its competition for new habitats. The results provided a reference for the management of non-native species, and the control of its invasive potential in Shenzhen Bay. References | Related Articles | Metrics

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Effects of Ecological Breeding under Forest on Plant Diversity and Soil Properties GAO Cheng-fang,LUO Xu-hui,ZHANG Xiao-pei,WENG Bo-qi 2017, 46(02): 137-141.  DOI: 10.3969/j.issn.1009-7791.2017.02.008 Abstract ( )   PDF (872KB) ( )   The forest stock, plant diversity, soil physical and chemical properties, and soil erosion modulus of ecological breeding under forest were studied. The results showed that forest stock of ecological breeding under forest was significantly higher than that of CK(P0.05). But soil moisture content, pH value, nitrate nitrogen and ammonium nitrogen were higher than those of CK. Soil erosion modulus of ecological breeding under forest was 120 t穔m-2 in downhill, compared to CK, total soil erosion decreased by 770 t穔m-2, the decreasing amplitude was up to 95.1%. References | Related Articles | Metrics

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Effects of Different Fertilization Treatments on Nitrogen Content and Its Distribution Pattern in Castanopsis fissa Seedlings SUN Li-jing,CHEN Hong-yue 2017, 46(02): 142-146.  DOI: 10.3969/j.issn.1009-7791.2017.02.009 Abstract ( )   PDF (885KB) ( )   To discuss nitrogen content and its distribution pattern of Castanopsis fissa seedlings under different nutritional conditions, different gradient ratios fertilizing of C. fissa seedlings were carried out by uniform design. The results showed that T5 treatments (N: 0.0750 g穝train-1, P2O5: 0.3500 g穚lant-1) was best for C. fissa seedlings growth. In each growth period, N contents of root, stem or leaf were varied with different nutritional conditions, the relationship between C. fissa seedlings total N content and N application rate was positive. Nitrogen content had a trend of transferring from root to leaf with the growth of C. fissa seedlings. The correlation analysis showed that nitrogen contents of root and stem, stem and leaf showed significantly positive correlation. Nitrogen contents of root and leaf showed positive correlation. References | Related Articles | Metrics

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Analysis on Growth Characteristics and Adaptation of Magnolia officinalis Based on Standard Deviation Classification Method of Normal Distribution SHI Xiang-dong 2017, 46(02): 147-151.  DOI: 10.3969/j.issn.1009-7791.2017.02.010 Abstract ( )   PDF (907KB) ( )   The growth of height and breast-diameter of forest stands of Magnolia officinalis at slop-bottom, slop-middle and slop-top were investigated by large sample. The results indicated that the distribution of height growth and breast-diameter growth at slop-bottom, slop-middle and slop-top were 4.3-14.1 m, 4.0-15.3 m, 4.3-14.5 m and 5.0-21.5 cm, 5.0-19.6 cm, 5.1-18.2 cm respectively. The growth types of height and breast-diameter of M. officinalis at different slop-position were classified by the standard deviation classification method. The results showed that the distribution of diversity growth types at different slop-position was consistent, and the colony weight of growth types with the average value ± 1σ was about 86%. The average values of growth of height and breast-diameter were different because of different site conditions of slop-position. The growth difference was observed from genetic diversity and adaption growth of M. officinalis, which would provide reference for excellent individual selection and provenance improvement. References | Related Articles | Metrics

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Effects of Gap Thinning on Growth and Form Quality of Taxus wallichina var. mairei Seedlings in Pinus massoniana Forest and Optimization KANG Yong-wu,OU Jian-de,LUO Ning 2017, 46(02): 152-157.  DOI: 10.3969/j.issn.1009-7791.2017.02.011 Abstract ( )   PDF (921KB) ( )   In order to clarify the effects of different forest gap size thinning on growth and form quality of Taxus wallichina var. mairei and the cultivation of precious timbers, 18 gap sample plots were established in Pinus massoniana forest after gap thinning in Mingxi County, Fujian, China to measure the indices of growth, stem form and branching indexs of 12-year old T. wallichina var. mairei plantations. The 18 sample were classified in gap size as follows: ClassⅠ, Ⅱ, and Ⅲ, which had a gap size of 50—100 m2, 100—150 m2, and 150—200 m2, respectively. The relationship between the gap size and growth, stem form and branching were investigated. The 18 gap sample plots were cluster analyzed according to the comprehensive score of growth, stem form and branching traits by principal component cluster analysis method. Determined the optimal gap sample plots group, estimate the optimal group gap size general parameter, and accurate optimization of the gap size of the gap thinning. The results showed that gap size significantly affected 10 indices, i.e. height, DBH, crown width, forking rate, stem straightness, stem fullness, taperingness, diameter height ratio, interval between branches, max-branch base diameter and comprehensive evaluation value. ClassⅠand Ⅱ both significantly promoted the growth of height, DBH, crown width and comprehensive evaluation value, and on the indices of stem form, both significantly inhibited max-branch base diameter. ClassⅠsignificantly inhibited forking rate and improved stem fullness. Class Ⅱ significantly reduced taperingness, improved stem straightness and diameter height ratio, both significantly increased intervals between branches. ClassⅠand Ⅱ significantly improved the comprehensive evaluation score of precious timbers. The optimum gap thinning was 105.31—153.21 m2 for gap size with a 95% confidence interval, which can greatly accelerate tree growth of forest and improve lumber quality. References | Related Articles | Metrics

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Effects of Cold Weather on Zingiberales Plants in Xiamen, China LIU Xue-xia 2017, 46(02): 158-162.  DOI: 10.3969/j.issn.1009-7791.2017.02.012 Abstract ( )   PDF (1256KB) ( )   Xiamen area was suffered a strong cold wave in January 22－27, 2016, causing cold damage on Zingiberales plants in Xiamen Botanical Garden. The temperature reached the lowest point at –2 ℃ in January 24. The damage degrees of the plants were divided into 6 levels. 38 species of Zingiberales plants were investigated. Only 4 species were not hurt in 38 species after the cold wave. Zingiberaceae and Strelitziaceae had higher resistance to cold wave, while Heliconiaceae and Costaceae were more damaged in the cold-air outbreak. The cold resistance of Alpinia was better than that of Heliconia and Costus. Results showed that the latitude of habitat was the major factor affecting the cold resistance of Zingiberales plants. Species from lower latitude were more damaged when suffered the cold wave. The elevation of habitat also affected the cold resistance of Zingiberales plants. References | Related Articles | Metrics

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Leaf Morphology of Mutated Impatiens balsamina Induced by Space Flight LIAO Xue-lan,LIAO Ke,WEN Guo-qin,WANG Mei,FENG Hao,ZHANG Li,PAN Yuan-zhi 2017, 46(02): 163-166.  DOI: 10.3969/j.issn.1009-7791.2017.02.013 Abstract ( )   PDF (9115KB) ( )   This study focused on the offspring of mutated Impatiens balsamina that was induced by space flight of Shenzhou 4. Comparative analysis of morphological characteristics of the mutant and normal I. balsamina (the control group) was performed. The results showed that, compared to the control group, the offspring of mutagenic I. balsamina variety changed greatly in ratio of leaf length to leaf width and leaf thickness. No significant difference in stomatal width was observed between the two groups, while their stomatal lengths varied significantly. Moreover, morphological mutations in leaves resulted in variations in detached leave wilting curve: the water loss was slower and steadier in mutants than in controls. The results suggested mutation in leaf morphology of mutated I. balsamina induced by space flight was beneficial. References | Related Articles | Metrics

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Studies on Morphology and Histology of Curcuma wenyujin Rhizoma from Hainan LIU Pei-wei,HUANG Xian,CHEN Xu-Yu,ZHANG Hui-feng,ZHANG Yu-xiu 2017, 46(02): 167-169.  DOI: 10.3969/j.issn.1009-7791.2017.02.014 Abstract ( )   PDF (1375KB) ( )   The morphological characteristics of Curcuma wenyujin rhizoma from Hainan and Wenzhou, Zhejiang, were observed by traditional paraffin section and photomicrographic technique. The results showed that the morphological characteristics of C. wenyujin rhizoma from Hainan and Wenzhou were consistent, and characters and microscopic identification of both were accorded with Chinese Pharmacopoeia (2015). Oil cell density of 2nd generation of C. wenyujin rhizoma from Hainan was higher than that of C. wenyujin from Wenzhou. References | Related Articles | Metrics

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Investigation of Wild Hygienical Plants in Guangdong Xiangtoushan National Nature Reserve CHEN Xin-gui,LUO Tan-ji,DENG Jie-ming,KANG Ning,LIU Cai-qin,ZHANG Yue 2017, 46(02): 170-174.  DOI: 10.3969/j.issn.1009-7791.2017.02.015 Abstract ( )   PDF (896KB) ( )   Eighty-four species of wild hygienical plants belonging to 56 genera in 39 families were identified in Guangdong Xiangtoushan national nature reserve. There were 3 species of gymnosperm belonging to 2 genera in 2 families, 75 species of Dicotyledoneae belonging to 50 genera in 34 families, 6 species of Monocotyledoneae belonging to 4 genera in 3 familes. The dominant families were Rosaceae(13 species), Moraceae(5 species), Aquifoliaceae(3 species), Caprifoliaceae(3 species), Papilionacea(3 species), Polygonaceae(3 species), Rubiaceae(3 species), Vacciniaceae(3 species) and Verbenaceae(3 species). The dominant genera were Rubus(6 species), Ilex(3 species), Lonicera(3 species), Smilax(3 species) and Vaccinium(3 species). The resource conditions, life type and using plot of wild hygienical plants from the nature reserve were analyzed. The development and utilization measures were put forward. References | Related Articles | Metrics

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Research on Linolenic Acid Resources from Actinidia Plants in China Based on Literature Analysis WANG Bing-qing,WANG Jian-xia,CHEN Gong-xi,XIAO Jia-wei,SONG Wang 2017, 46(02): 175-180.  DOI: 10.3969/j.issn.1009-7791.2017.02.016 Abstract ( )   PDF (916KB) ( )   The species and distribution of Actinidia enriched in α-linolenic acid, content and extraction methods of α-linolenic acid in Actinidia were summarized. In China, there were 11 species and 10 cultivars of Actinidia enriched in α-linolenic acid. Most species distributed in Yangtze River, only a few in northeast and southwest regions of China. Seed oil of Actinidia showed relative high content of α-linolenic acid (about 40%—70% of total fatty acids). Importantly, different extraction methods had great influence on extraction effect of α-linolenic acid. The content of α-linolenic acid separated and purified by Urea adduction fractionation and molecular distillation was up to 87.20%. References | Related Articles | Metrics

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A New Recorded Spermatophyte Family (Petrosaviaceae) from Jiangxi and Its Biogeographic Implication ZHANG Zhong,ZHAO Wan-yi,FAN Qiang,CHEN Chun-quan,LIAO Wen-bo 2017, 46(02): 181-184.  DOI: 10.3969/j.issn.1009-7791.2017.02.017 Abstract ( )   PDF (2022KB) ( )   The family Petrosaviaceae, containing one genus and one species, i.e. Petrosavia Beccari and Petrosavia sakurai (Makino) J. J. Smith ex van Steenis, was reported as a new record to Jiangxi Province, China. The new record taxon was a residual species that reflected the paleo-climate and tropical paleo-forest at the late tertiary. And it was discovered in the montane evergreen broadleaved forest (elevation 1200 m) in Bijiashan in Jinggangshan Region, which could indicate strongly a remnant property of the monsoon rainforest in Jinggangshan region. References | Related Articles | Metrics

Plant cultivation

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Effects of IBA and NAA on Cutting Propagation of Rhodomyrtus tomentosa YE Chang-hui,CHEN Ting-hui,CHEN Heng-heng,ZHENG Xiao-ting,CHEN Dan,WU Tian 2017, 46(02): 185-187.  DOI: 10.3969/j.issn.1009-7791.2017.02.018 Abstract ( )   PDF (4773KB) ( )   Using binary quadratic regression general rotational combination, the effects of plant growth regulators (IBA and NAA) on Rhodomyrtus tomentosa root development by cuttage with treatments of different concentrations of IBA or NAA were investigated. The results suggested that both IBA and NAA played positive roles on root development during cutting propagation of R. tomentosa. Compared with NAA, IBA exerted a more significant function on rooting rate, root length and weight. The optimum concentration was IBA 100 mg稬-1and NAA 100 mg稬-1, which was beneficial to survival rate of cutting propagation of R. tomentosa. References | Related Articles | Metrics

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Effects of Fertilizers on Growth and Flower quality of Phalaenopsis DING Xue,LING Jun-hua,LIN Xue-fen,YE Yin-zhu,NI Jian-zhong,HU Yan-hui,HE Zhong-jian 2017, 46(02): 188-191.  DOI: 10.3969/j.issn.1009-7791.2017.02.019 Abstract ( )   PDF (3812KB) ( )   In order to improve flower quality of Phalaenopsis, the effects of different fertilizers on growth and flower quality of Phalaenopsis ‘Sogo China Red’ and Phal. ‘Bull Demon King’ were investigated. The results showed that the characters of Phalaenopsis with T1 and T2 treatments were better than CK, but the influence of flower quality between T1 and CK was not significant. The nutrient solution of monomer fertilizer(N∶P∶K=1∶3∶2) showed a significantly impact on the leaf size, leaf chlorophyll content, flower stalk and the number of flower, and it was contributed to commercial value of Phalaenopsis . References | Related Articles | Metrics

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Cultivation Techniques of Tissue Culture Container Seedlings of Asplenium nidus WANG Chang-shui 2017, 46(02): 192-194.  DOI: 10.3969/j.issn.1009-7791.2017.02.020 Abstract ( )   PDF (815KB) ( )   Primary culture, subculture, rooting culture and transplantation of Asplenium nidus spores were carried out in this study. Results showed that the best primary culture medium was MS＋0.1% activated carbon, the formation rate of prothallus was 80%; In the multiplication medium with 1/3MS ＋6-BA 1.8 mg·L-1＋NAA 0.5 mg·L-1, the proliferation times was up to 5.1; in rooting medium with 1/2MS＋NAA 0.5 mg·L-1＋IBA 0.5 mg·L-1, the rooting rate of subculture shoot was highest; the best transplanting substrate was the red soil＋humus (1∶1), transplanting survival rate was 90%. References | Related Articles | Metrics

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Research Advance on Plant Response to Low Temperature Stress and Its Ubiquitination ZHOU Qiang,HAN Ying-ying 2017, 46(02): 195-200.  DOI: 10.3969/j.issn.1009-7791.2017.02.021 Abstract ( )   PDF (868KB) ( )   Cell regulation mechanism is the main response of plant to low temperature stress. In this article, the effects of low temperature stress on signal transduction, gene expression, ubiquitination, the level of proline, soluble sugar and SOD enzyme activity were discussed, so as to provide references for studying on plant resistance to low temperature or freezing stress. References | Related Articles | Metrics