Abstract: The buds of Hypoxis angustifolia were used as material for tissue culturing. The results showed that the optimum culture medium for bud callus induction was MS + 6-BA 0.8 mg·L-1 + NAA 0.05 mg·L-1 + TDZ 0.1 mg·L-1; the culture medium for bud induction was MS + 6-BA 0.5 mg·L-1 + NAA 0.02 mg·L-1 + AC 0.2 g·L-1; the culture medium for propagation was MS + 6-BA 1.0 mg·L-1 + NAA 0.1 mg·L-1 + Huabao-1 1 g·L-1 + AC 0.2 g·L-1. And the subculture circle was 30 d, the multiplication rate was 10-20. The culture medium for root was 1/2 MS + IBA 0.5 mg·L-1 + NAA 0.02 mg·L-1 +AC 0.5 g·L-1. The suitable transplanting medium was surface soil and vermiculite (3:1), the survival rate reached 80%.

Key words: Hypoxis angustifolia, bud, propagation, tissue culture

摘要： 以狭叶小金梅草(Hypoxis angustifolia)当年花苞为材料进行离体快速繁殖体系研究。结果表明，花苞的愈伤诱导适宜培养基为MS + 6-BA 0.8 mg·L-1 + NAA 0.05 mg·L-1 + TDZ 0.1 mg·L-1；诱导芽培养基为MS + 6-BA 0.5 mg·L-1 + NAA 0.02 mg·L-1 + AC(活性碳) 0.2 g·L-1；芽继代增殖培养基为MS + 6-BA 1.0 mg·L-1+ NAA 0.1 mg·L-1 + 花宝1号1 g·L-1 + AC 0.2 g·L-1，30 d为一个继代周期，增殖系数达10～20倍；壮苗生根培养基为1/2MS + IBA 0.5 mg·L-1 + NAA 0.02 mg·L-1 + AC 0.5 g·L-1。将生根苗移栽至泥土:蛭石按3:1配制旳基质中，盖膜保湿，成活率达80%。

关键词: 狭叶小金梅草, 花蕾, 增殖, 离体培养