Chemical Constituents and Antibacterial and Anti-inflammatory Activities of Ethanol Extracts from Cinnamomum burmanni Leaves

doi:10.3969/j.issn.1009-7791.2023.05.002

Subtropical Plant Science ›› 2023, Vol. 52 ›› Issue (5): 381-390.DOI: 10.3969/j.issn.1009-7791.2023.05.002

• Plant physiology, biochemistry and molecular biology • Previous Articles Next Articles

Chemical Constituents and Antibacterial and Anti-inflammatory Activities of Ethanol Extracts from Cinnamomum burmanni Leaves

SU Xin-ke¹, YAO Min¹, YANG Lei¹, WU Peng¹, JIN Jing-wei¹, XIONG Ping², HUANG Jun-ming^1*, LI Dong-li¹

(1. School of Biotechnology and Health Sciences, Wuyi University, Jiangmen 529000, Guangdong China; 2. Department of Pharmaceutical Engineering, South China Agricultural University, Guangzhou 510642, Guangdong China)

Received:2023-04-05 Accepted:2023-06-01 Online:2023-10-30 Published:2024-01-19
Contact: HUANG Jun-ming

阴香叶乙醇提取物的化学成分鉴定及其抗菌抗炎活性

苏馨科¹，姚敏¹，杨磊¹，吴鹏¹，金静维¹，熊平²，黄俊明^1*，李冬利¹

(1. 五邑大学生物科技与大健康学院, 广东江门 529000；2. 华南农业大学制药工程系，广东广州 510642)

通讯作者: 黄俊明
基金资助:
广东省企业科技特派员专项(GDKTP20211049000)；2021年广东省科技创新战略专项资金(“大专项+任务清单”) (2021A0105)

Abstract

Abstract: Modern separation techniques such as D101 macroporous resin, MCI macroporous resin, positive and negative silica gel, gel column chromatography and semi-preparative high performance liquid chromatography were used to separate, purify and identify the chemical components in 95% ethanol extracts of Cinnamomum burmanni leaves from Meizhou, Guangdong Province. At the same time, the antibacterial activities of the compounds were determined by microplate method, and their anti-inflammatory activities were determined by LPS-induced BV2 cells. The results showed that 25 compounds were isolated and identified from the 95% ethanol extracts of C. burmanni leaves, including flavonoids (1–3), lignans (4–6), simple phenylpropanoids (7–16), coumarins (18), benzoquinones (20–21), sesquiterpenes (22–24) and indole alkaloids (25). Except for compounds 1, 2, 5 and 18, all of them were isolated from C. burmanni for the first time. The results of antibacterial experiments showed that compounds 20 and 21 showed good antibacterial activity against Staphylococcus aureus, with MIC of 32 μmol·L^–1, while MBC of compound 20 was 64 μmol·L^–1, and MBC of compound 21 was 32 μmol·L^–1. The results of antibacterial kinetics also showed that compound 21 had better antibacterial activity than compound 20. The results of anti-inflammatory experiments showed that compounds 5, 6 and 7 could inhibit the production of NO in BV2 cells induced by LPS, and showed good anti-inflammatory activity, the IC₅₀ values were 7.85 μmol·L^–1, 24.97 μmol·L^–1 and 7.49 μmol·L^–1, respectively.

Key words: Cinnamomum burmanni, chemical composition, antibacterial activity, anti-inflammatory activity

摘要： 采用D101大孔树脂、MCI小孔树脂、正反相硅胶、凝胶柱层析和半制备型高效液相色谱等分离技术对广东梅州产阴香Cinnamomum burmanni叶95%乙醇提取物的化学成分进行分离纯化和结构鉴定，用微孔板法测定化合物抗菌活性，以LPS诱导的BV2细胞测定化合物抗炎活性。结果表明，从阴香叶95%乙醇提取物中分离鉴定了25个化合物，包括黄酮类化合物(1～3)、木脂素类化合物(4～6)、简单苯丙素类化合物(7～16)、香豆素类化合物(18)、苯醌类化合物(20～21)、倍半萜类化合物(22～24)和吲哚生物碱(25)，除化合物1、2、5、18以外，其余都是首次从阴香中分离得到。化合物20和21对金黄色葡萄球菌表现出较好的抑菌活性，MIC均为32 μmol·L^–1，化合物20的MBC为64 μmol·L^–1，化合物21的MBC为32 μmol·L^–1。抑菌动力学实验也表明，化合物21的抗菌活性优于化合物20。化合物5、6、7可抑制LPS诱导的BV2细胞NO的生成，表现出较好的抗炎活性，IC₅₀值分别为7.85 μmol·L^–1、24.97 μmol·L^–1、7.49 μmol·L^–1。

关键词: 阴香, 化学成分, 抗菌活性, 抗炎活性

CLC Number:

Q946

SU Xin-ke, YAO Min, YANG Lei, WU Peng, JIN Jing-wei, XIONG Ping, HUANG Jun-ming, LI Dong-li. Chemical Constituents and Antibacterial and Anti-inflammatory Activities of Ethanol Extracts from Cinnamomum burmanni Leaves[J]. Subtropical Plant Science, 2023, 52(5): 381-390.

苏馨科, 姚敏, 杨磊, 吴鹏, 金静维, 熊平, 黄俊明, 李冬利. 阴香叶乙醇提取物的化学成分鉴定及其抗菌抗炎活性[J]. 亚热带植物科学, 2023, 52(5): 381-390.

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Chemical Constituents and Antibacterial and Anti-inflammatory Activities of Ethanol Extracts from Cinnamomum burmanni Leaves

阴香叶乙醇提取物的化学成分鉴定及其抗菌抗炎活性

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