Abstract: In order to provide scientific basis for the introduction of excellent cold-resistant rootstocks in Gansu, the branches of 12 grape rootstock varieties treated with artificial simulated low temperature were used as test materials, and the relative conductivity, proline, malondialdehyde, soluble sugar and soluble protein contents of the branches were determined. The relative conductivity combined with the Logistic equation was used to predict the half-lethal temperature (LT50) of each variety, and the subordinate function method was applied to make a comprehensive evaluation of the cold resistance of grape rootstocks. The results showed that the relative conductivity, soluble protein, soluble sugar, malondialdehyde, and free proline contents of the branches of each grape rootstock increased gradually, and the calculated half-lethal temperatures (LT50) ranged from -16.32 ℃ to -24.84 ℃; and the results of the comprehensive evaluation showed that the strengths of cold resistance of 12 grape varieties rootstocks were in the order of Beda, Shanhe 2, 101-14, 5BB, 188-08, SO4, Valliant, 3309, 1103P, Du Lot, BRNo.2, 110R.

Key words: grape rootstock, cold resistance, low temperature stress, subordinate function

摘要： 采用人工模拟低温处理的12个葡萄砧木品种枝条为材料，测定枝条相对电导率及脯氨酸、丙二醛、可溶性糖、可溶性蛋白含量，基于相对电导率和Logistic方程预测各品种的半致死温度(LT50)，并运用隶属函数法对其抗寒性进行综合评价，为甘肃引进抗寒性优良葡萄砧木提供科学依据。结果表明，各品种砧木枝条的相对电导率、可溶性蛋白、可溶性糖、丙二醛、游离脯氨酸含量均随温度降低逐渐增加，半致死温度(LT50)介于-16.32 ℃～-24.84 ℃；综合评价表明，12个葡萄品种砧木的抗寒性强弱依次为贝达、山河2号、101-14、5BB、188-08、SO4、Valliant、3309、1103P、Du Lot、BRNo.2、110R。

关键词: 葡萄砧木, 低温胁迫, 抗寒性, 隶属函数