Abstract: By using PLB and tube seedlings as material, based on the culture medium MS + 6-BA 2 mg/L + Ad 1 mg/L + NAA 0.1 mg/L + sucrose 30 g/L, the effects of 5 additives including banana, taro, potato, pumpkin, activated carbon (AC) and their combinations on the Oncidium ‘Gower Rramsey’ protocorm-like body (PLB) propagation and tube seedlings growth, were studied by the method of morphological indexes by weighted analysis. The results showed that potato homogenate 50 g/L appropriate for PLB propagation, after 45 d of cultured, the PLB propagation coefficient was 3.64 times than the control; banana homogenate 50 g/L + AC 1 g/L appropriate for small tube seedlings (about 1.5 cm) propagation and growth, weighted score was 1.53 times than the control; potato homogenate 25 g/L + banana homogenate 25 g/L appropriate for middle tube seedlings (about 2.5 cm) propagation and growth, weighted score was 1.55 times than the control.

Key words: Oncidium, tissue culture, protoeorm-like body (PLB), tube seedling, propagation, additives

摘要： 以文心兰切花品种‘南茜’原球茎(PLB)及试管苗为材料，在培养基MS＋6-BA 2 mg/L＋Ad 1 mg/L＋NAA 0.1 mg/L＋蔗糖30 g/L基础上，利用增殖系数及不同形态指标加权分析研究马铃薯、芋头、南瓜、香蕉及活性炭(AC)5种培养基添加物及其组合对‘南茜’PLB增殖及试管苗生长的影响。结果表明，适宜文心兰PLB增殖培养的添加物为马铃薯泥50 g/L，培养45 d后，PLB增殖系数是对照的3.64倍；适宜无菌小苗(约1.5 cm)增殖及生长的添加物为香蕉泥50 g/L＋AC 1 g/L，加权总分是对照的1.53 倍；适宜无菌中苗(约2.5 cm)增殖及生长的添加物为马铃薯泥25 g/L＋香蕉泥25 g/L，加权总分是对照的1.55倍。

关键词: 文心兰, 组织培养, PLB, 试管苗, 增殖, 添加物