Abstract: The Hibiscus rosa-sinensis leaves were used as the meterials, and the response surface methodology was used to optimize the flavonoids extraction process, which was designed by Box-Behnken. The results showed that the optimum conditions for extracting flavonoids from H. rosa-sinensis leaves was: the ethanol concentration 70%, 70 min ultrasound treatment and the ratio of solid 40:1. Under the optimum conditions, the yield of flavonoids was 34.56 mg/g.

Key words: Hibiscus rosa-sinensis, leaf, flavonoids, response surface methodology

摘要： 以扶桑叶为研究材料，以Box-Behnken中心组合实验设计，利用响应面法对扶桑叶黄酮提取工艺进行优化。结果表明，超声波辅助乙醇提取扶桑叶黄酮优化工艺条件为乙醇浓度70%，超声提取时间70 min，液料比40∶1。在最佳工艺条件下扶桑叶黄酮提取的得率最高，为34.56 mg/g。

关键词: 扶桑叶, 黄酮, 响应面法