Subtropical Plant Science ›› 2001, Vol. 30 ›› Issue (02): 16-19.
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SHEN Yu-fen
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沈育芬
作者简介:
Abstract: The Griseofulvin producing strain D-756 was taken as the original strain. After three-generation treatment with UV and LiCl, the mutant strain F-1012 of resistant chloride was obtained. The results of the morphological character, yield and the resistance to chloride character of the mutant strain showed that the yield of the mutant strain increased 34.5% when the concentration of chloride in the fermentation medium was raised from 1.5% to 2.0%, and when the concentration of chloride in solid medium was raised to 11%, the survival ratio of the mutant strain spore was 25.5% higher than that of the original strain spore.
Key words: Griseofulvin, mutation, morphological character, yield, resistance to chloride
摘要: 以灰黄霉素产生菌D-756为出发菌株,经过三代紫外线+氯化锂诱变处理,获得耐氯变株F-1012,该变株在形态特征及产量、耐氯性等方面均发生变化。当发酵培养基中的氯化物浓度由1.5%提高到2.0%,F-1012的大米孢子效价提高了34.5%;当固体平板培养基中氯化物浓度提高到11%时,F-1012的孢子存活率比出发菌株提高了25.5%。
关键词: 灰黄霉素, 诱变, 形态特征, 产量, 耐氯特性
CLC Number:
Q933
SHEN Yu-fen. Effects of mutation of Griseofulvin producing strain[J]. Subtropical Plant Science, 2001, 30(02): 16-19.
沈育芬. 灰黄霉素产生菌的诱变效应[J]. 亚热带植物科学, 2001, 30(02): 16-19.
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