Abstract: Onosma Paniculatum treated with He-Ne laser and magnetic field of certain intensity was studied. The results showed that the pigment content was higher after 2~3h laser radiation and the cell growth and pigment formation could be reduced in 1.0 T magnetic field. The two kinds of physical factors had no obvious influence on Onosma paniculatum pigment composition by the chromatographic analysis with thin-layer silica gel.

Key words: Onosma paniculatum, He-Ne laser, magnetic field, tissue culture, callus, shikonin

摘要： 用He-Ne激光和一定强度的磁场处理滇紫草愈伤组织,发现2~3h激光辐照可提高色素含量;而1.0T磁场能促进细胞生长和色素形成。硅胶薄层层析比较两种物理因子对紫草色素成分无显著影响。

关键词: 滇紫草, He-Ne激光, 磁场, 组织培养, 愈伤组织, 紫草色素