Abstract: The effect of different concentrations of KT and 2,4-D on redifferentiation of Anemarrhena asphodeloides callus was studied. The results showed that the optimal regenerated shoot medium, the optimal seedling growth medium and the optimal proliferation medium of A. asphodeloides callus were all MS + KT 4.0 mg/L. The rooting of A. asphodeloides callus was both inhibited by using KT alone or using KT and 2, 4-D altogether.

Key words: Anemarrhena asphodeloides, callus, KT, 2,4-D, redifferentiation

摘要： 本文探讨KT和2,4-D组合对知母愈伤组织再分化的影响。结果表明：知母愈伤组织再生芽和促进再生苗长高以及愈伤组织增殖的最佳培养基均为MS + KT 4.0 mg/L。单独使用KT或KT与2,4-D组合均可抑制知母愈伤组织生根。

关键词: 知母, 愈伤组织, KT, 2, 4-D, 再分化