Studies on the Determination of Chemical Constituents and Bioactivities in Seeds of Voacanga africana

doi:10.3969/j.issn.1009-7791.2014.02.002

Subtropical Plant Science ›› 2014, Vol. 43 ›› Issue (02): 98-102.DOI: 10.3969/j.issn.1009-7791.2014.02.002

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Studies on the Determination of Chemical Constituents and Bioactivities in Seeds of Voacanga africana

LIU Shao-song,ZHENG Zhi-zhong,CHEN Liang-hua,KANG Lian-chang,TONG Qing-xuan,ZHENG Yuan-bin,MING Yan-lin

(1.Xiamen Botanical Plant Introduction, Quarantine and Plant Products Key Laboratory, Research and Development Center for Medicinal Plants and Plant Drugs, Xiamen Overseas Chinese Subtropical Plant Introduction Garden, Xiamen 361002, Fujian China; 2.Enjoye C&G (Xiamen) Bioengineering Co. Ltd, Xiamen 361022, Fujian China)

Received:2014-02-21 Revised:2014-02-21 Online:2014-06-10 Published:2014-06-10
Contact: MING Yan-lin

非洲马铃果种子的化学成分含量测定和生物活性研究

刘韶松,童庆宣,郑志忠,陈良华,康炼常,郑远斌,明艳林

（1.厦门华侨亚热带植物引种园药用植物与植物药研发中心/厦门市植物引种检疫与植物源产物重点实验室，福建厦门 361002；2.天医堂(厦门)生物工程有限公司，福建厦门 361022）

通讯作者: 明艳林
作者简介:刘韶松，助理研究员，从事天然产物分离鉴定和活性研究。
基金资助:
厦门市科技创新资金项目(3502Z20101016; 3502Z 20122011)

Abstract

Abstract: In this study, the total alkaloids, total flavonoids and water-soluble polysaccharides of seeds of Voacanga africana native to Ghana were determined by means of spectrophotometry, cytotoxicity and antioxidant activity of different isolation from ethanol extract by MTT and DPPH method, respectively. The results revealed that the content of total alkaloids, total flavonoids and water-soluble polysaccharides were 3.04%, 0.41% and 3.04%, respectively. The alkaloid isolation exhibited strong cytotoxity against the human hepatocarcinoma cells SMMC7721, the human gastric carcinoma cells SGC7901 and human normal liver cells Chang-liver and the IC₅₀values were 11.99±2.22, 8.72±6.11 and 7.31±4.39 μg·mL^-1 respectively. The n-butanol isolation displayed antioxidant activity and the IC₅₀ value was 0.268?0.002 mg.mL^-1

Key words: Voacanga africana, content determination, cytotoxity, antioxidant activity, DPPH method

摘要： 以分光光度法测定非洲马铃果种子总生物碱、总黄酮、水溶性多糖含量，MTT法测定乙醇提取物不同分离部位的细胞毒活性，以及DPPH法测定不同分离部位的抗氧化活性。结果表明，非洲马铃果种子总生物碱、总黄酮、水溶性多糖含量分别为5.70%、0.41%和3.04%；各分离部位中总生物碱的细胞毒活性最强，其对人的肝癌细胞株SMMC7721、胃癌细胞株SGC7901和正常肝细胞株Chang-liver的IC₅₀值分别为11.99±2.22、8.72±6.11和7.31±4.39 μg·mL^-1；各分离部位中正丁醇相抗氧化活性最强，IC50值为0.268±0.002 mg·mL^-1。

关键词: 非洲马铃果, 含量测定, 细胞毒活性, 抗氧化活性, DPPH法

CLC Number:

R284.1

LIU Shao-song,ZHENG Zhi-zhong,CHEN Liang-hua,KANG Lian-chang,TONG Qing-xuan,ZHENG Yuan-bin,MING Yan-lin. Studies on the Determination of Chemical Constituents and Bioactivities in Seeds of Voacanga africana[J]. Subtropical Plant Science, 2014, 43(02): 98-102.

刘韶松,郑志忠,陈良华,康炼常,童庆宣,郑远斌,明艳林. 非洲马铃果种子的化学成分含量测定和生物活性研究[J]. 亚热带植物科学, 2014, 43(02): 98-102.

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Studies on the Determination of Chemical Constituents and Bioactivities in Seeds of Voacanga africana

非洲马铃果种子的化学成分含量测定和生物活性研究

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