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10 June 2014, Volume 43 Issue 02 Previous Issue    Next Issue

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Construction of Normalized Full-length cDNA Library for the Coralloid Roots of Cycas debaoensis and Analysis of its Expressed Sequence Tags WANG Yun-hua,CHEN Ting,LI Nan 2014, 43(02): 93-97.  DOI: 10.3969/j.issn.1009-7791.2014.02.001 Abstract ( )   PDF (349KB) ( )   A normalized full-length cDNA library was constructed with the coralloid roots of Cycas debaoensis by DSN (duplex-specific nuclease) normalization method combined with SMART (Switching Mechanism At 5′ end of the RNA Transcript) technique. The titer of original cDNA library was about 1.5×106 cfu·mL-1 and the average insertion size was about 1 kb with high recombination rate (97%). The 175 high quality expressed sequence tags (ESTs) were obtained from 192 randomly picked cDNA clones. Clusting and assembly of ESTs resulted in 165 unique sequences, consisting of 7 contigs and 158 singlets. Using a generic similarity threshold (score ≥ 200, identity ≥ 60), approximately 115 (66.1%) of all 174 clean ESTs matched corresponding homologous sequences according to BLAST analysis of the nr-nt (non-redundant protein and non-redundant nucleotide sequence) database in GenBank. The 33.9% (59) ESTs have no significant match. These results showed preliminarily that we successfully constructed a normalized full-length cDNA library of coralloid roots in C. debaoensis, which could serve as an abundant information platform for functional marker development and functional gene study. References | Related Articles | Metrics

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Studies on the Determination of Chemical Constituents and Bioactivities in Seeds of Voacanga africana LIU Shao-song,ZHENG Zhi-zhong,CHEN Liang-hua,KANG Lian-chang,TONG Qing-xuan,ZHENG Yuan-bin,MING Yan-lin 2014, 43(02): 98-102.  DOI: 10.3969/j.issn.1009-7791.2014.02.002 Abstract ( )   PDF (297KB) ( )   In this study, the total alkaloids, total flavonoids and water-soluble polysaccharides of seeds of Voacanga africana native to Ghana were determined by means of spectrophotometry, cytotoxicity and antioxidant activity of different isolation from ethanol extract by MTT and DPPH method, respectively. The results revealed that the content of total alkaloids, total flavonoids and water-soluble polysaccharides were 3.04%, 0.41% and 3.04%, respectively. The alkaloid isolation exhibited strong cytotoxity against the human hepatocarcinoma cells SMMC7721, the human gastric carcinoma cells SGC7901 and human normal liver cells Chang-liver and the IC50 values were 11.99±2.22, 8.72±6.11 and 7.31±4.39 μg·mL-1 respectively. The n-butanol isolation displayed antioxidant activity and the IC50 value was 0.268?0.002 mg.mL-1 References | Related Articles | Metrics

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Studies on the Stability of Phycocyanion of Rhodella reticulata ZHANG Yan-yan,CHEN Bi-lian,CHEN Zhang-jie 2014, 43(02): 103-107.  DOI: 10.3969/j.issn.1009-7791.2014.02.003 Abstract ( )   PDF (368KB) ( )   The unicellular red alga, Rhodella reticulata, can synthesize some bioactive substances such as phycobiliprotein and exopolysaccharides. After extraction by fractional precipitation with ammonium sulfate, phycocyanion (PC) was purified by ion-exchange chromatography on DEAE-Sepharose Fast Flow column. In this paper, effects of various parameters such as pH, temperature, illumination, food additives and metal ions on the stability of PC of Rhodella reticulate were studied. PC has the most stable activity in pH 7; Lower temperature is in favour of the activity of PC, so do appropriate amount of sucrose and glucose; It had higher stability away from light; Metal ions affect the stability of PC. References | Related Articles | Metrics

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Polyphenols Extraction from Pyrrosia petiolosa by Ultrasonic and Its Content Determination CHENG Dan-dan,GAO Bei,XIN Xiao-wei,GAO De-min 2014, 43(02): 108-109.  DOI: 10.3969/j.issn.1009-7791.2014.02.004 Abstract ( )   PDF (224KB) ( )   To optimize the extraction of total polyphenols in Pyrrosia petiolosa by ultrasonic, the content of the polyphenols was used as investigation index, and the total polyphenol content was determined by Folin-Ciocalteus colorimetric. The extraction process parameters for polyphenols were optimized by the single factor and the orthogonal experiment. The results showed that there was a good linearity between gallic acid concentration and absorbance in the range 1~10 mg·L-1 (y = 2.406x －0.012, R= 0.9994)；The optimum conditions were as follows: solvent concentration 55%, ultrasound time 60 min, extraction temperature 75 ℃, solid to liquid ratio 1∶10. The polyphenols extraction rate was 21.47 mg·g-1 in the best technical conditions. References | Related Articles | Metrics

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Effects of Plant Growth Regulators on Seed Germination of Dalbergia odorifera LIN Wei,LI Yun,HUANG Yong-fang,GONG Hai-guang,YANG Ya-hui,ZHA Qian-hui 2014, 43(02): 110-113.  DOI: 10.3969/j.issn.1009-7791.2014.02.005 Abstract ( )   PDF (221KB) ( )   Seeds of Dalbergia odorifera were treated by three plant growth regulators of 6-BA, IBA, GA3 and distilled water. The content of soluble sugar, starch, protein and fat of seeds were determined during germination. The results showed as follows：seeds of D. odorifera showed a downward trend in the soluble sugar content in all treatments. The starch content dropped from 0.6% which was the one of the third day to 0.2% below when the seeds ended germination. In the early period of germination (3～6 d), starch content of seeds changed little with the treatment of 6-BA, but there was a significant downward trend in the later period of germination. Changes of protein and fat content were relatively stable, and inapparent after treatment of three plant growth regulators. References | Related Articles | Metrics

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Structural and Histochemical Observation on the Anthers of Celosia argentea GUO Jiao-jiao,GUO Xiao-juan,SONG Tie-dan,TIAN Hui-qiao 2014, 43(02): 114-118.  DOI: 10.3969/j.issn.1009-7791.2014.02.006 Abstract ( )   PDF (382KB) ( )   Structural and histochemical observations on the developing anthers of Celosia argentea L. (Amaranthaceae) were conducted. Its microsporogenesis is simultaneous and microspore tetrad is tetrahedral. Its anther wall consists of four typical layers of cells and tapetum is homotypical. Its mature pollen contains a small generative cell and a vegetative cell, belonging to bicellular. In young anthers starches and lipids were few. At late microspore stage, degenerating tapetal cells transformed into large lipids, and at early bicellular pollen stage, middle layer cell also transformed into lipids. In early bicellular pollen polysaccharose appeared first, and then lipids. Mature pollen of C. argentea accumulated numerous starches and fewer lipids as storage material. References | Related Articles | Metrics

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Meiotic Observations on the Pollen Mother Cells of Five Interspecific Hybrids in Magnoliaceae ZHAO Qiang-min,HE Zi-can,WANG Ya-ling 2014, 43(02): 119-122.  DOI: 10.3969/j.issn.1009-7791.2014.02.007 Abstract ( )   PDF (329KB) ( )   The meiosis processes of pollen mother cells (PMCs) of five hybrids in Magnoliaceae were observed. The results were shown as follow: the meiosis process was quite normal; some bivalences separated early and formed univalent in MI; there were heteromorphic bivalent in PMCs, which revealed that certain differentiation existed among these parents, but only on the level of genes. The meiosis process is regular and can produce normal spores. On the other word, some nature hybrids can reproduce by themselves in wild, and will form a new species with certain population. References | Related Articles | Metrics

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Relationships among 3 Species of Dendrobium Based on RAPD Marker ZHANG Yang,CHEN Zhi-kuan,LAI Yu-bo,HUANG Shu-lin,SHAO Hong-wei, 2014, 43(02): 123-126.  DOI: 10.3969/j.issn.1009-7791.2014.02.008 Abstract ( )   PDF (318KB) ( )   Three species of Dendrobium, D. nobile, D. officinale and D. devonianum were analyzed using random amplified polymorphic DNA (RAPD) markers. A total of 113 bands were amplified by 10 RAPD primers, among which 106 (93.81%) bands were polymorphic. The data of 113 RAPD bands were used to generate genetic distances and to construct a dendrogram by unweighted pair group method analysis (UPGMA). The results showed that the RAPD approach was efficient in studying genetic background and relationships among these 3 species and could be used to provide abundant molecular data for the medicinal development and utilization of Dendrobium. References | Related Articles | Metrics

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The New Records of the Vascular Plants from Jiulianshan Nature Reserve, Jiangxi Province XU Guo-liang 2014, 43(02): 127-132.  DOI: 10.3969/j.issn.1009-7791.2014.02.009 Abstract ( )   PDF (935KB) ( )   Fifteen plant species are reported as new records to Mount Jiulianshan, among them, six species are newly reported to Jiangxi province including Gastrochilus japonicus (Makino) Schlechter, Chiloschista guangdongensis Z H Tsi, Dendrobium densiflorum Wallich, Sciaphila ramosa Fukuyama & T Suzuki, Burmannia nepalensis (Miers) J D Hooker, Hetaeria cristata Blume. References | Related Articles | Metrics

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Liparis gigantea, a New Record of Liparis from Fujian Province, China WANG Xiao-xia 2014, 43(02): 133-134.  DOI: 10.3969/j.issn.1009-7791.2014.02.010 Abstract ( )   PDF (176KB) ( )   Liparis gigantea, a newly recorded species of Orchidaceae from Fujian, is described. This species resembles L. nervosa, but differs by having bigger flowers, dorsal sepal 1.8—2 cm , flowers dark purple throughout the latter features smaller flowers, dorsal sepals shorter than 1.6 cm, flowers greenish, tinged with purple, never dark purple throughout. References | Related Articles | Metrics

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Changes in Floristic Composition and Species Diversity of Plant Community along Environment Gradient in the Yellow River Delta National Natural Reserve, China LI Xin-hua,WANG Yong,ZHU Zhen-lin 2014, 43(02): 135-138.  DOI: 10.3969/j.issn.1009-7791.2014.02.011 Abstract ( )   PDF (282KB) ( )   The typical wetlands in the Yellow River Delta National Natural Reserve had been selected to research on the variation rules of plant community composition and diversity along the environment gradient explored. The results showed that from the community of Ass. Phragmites australis-Typha angustifolia to Ass. Suaeda salsa, (1) the species richness fluctuated and there was the highest species richness in the community of Ass. Salix matsudana-Phragmites australis-Imperata cylinddrica var. major, while there was the lowest species richness in the community of Ass. Suaeda salsa; (2) α diversity of the community had a different fashion compared with species richness, and the Shannon-Winner diversity and Pelou evenness index had a pattern of fluctuated downward trend, while Simpson dominance index showed a pattern of fluctuating upward trend; (3) the changes of the β diversity pattern were not the same, and Jaccard index showed a fluctuated change, while the Copy index had a pattern of fluctuated downward trend. The changes of typical community composition and species richness in the Yellow River Delta wetlands were mainly related to the changes of habitats. References | Related Articles | Metrics

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Study on the Diversity of Machilus versicolora Community in Mangdang Mountain, Fujian Province HUANG Zi-liang,YANG Wan-ping,CHEN Xue-fu 2014, 43(02): 139-143.  DOI: 10.3969/j.issn.1009-7791.2014.02.012 Abstract ( )   PDF (274KB) ( )   By investigating the diversity of Machilus versicolora community in Mangdang Mountain and comparing with other adjacent communities, the results showed that the index of species diversity (ISD) in the M. versicolora community distinguished from evergreen broad-leaved forest located in the east middle sub-tropical zone China, which the rich of diversity of community showed shrubs, trees and herbs respectively, were richer than other adjacent communities without obvious change along latitudinal gradient. References | Related Articles | Metrics

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Study on the Tree Species, Distribution and Growth Status on Gulangyu Islet FANG Shui-chi 2014, 43(02): 144-151.  DOI: 10.3969/j.issn.1009-7791.2014.02.013 Abstract ( )   PDF (425KB) ( )   Using method of route reconnaissance and focus of the investigation, a study on tree species, distribution and growth was carried out on Gulangyu Islet in Xiamen City. The results showed that the trees in Gulangyu Islet?(including woody lianas, according to Engler system) were a total of 75 families and 296 species?(including subspecies,variants,cultivated varieties),? in which there were primary section of 31 families, 49 species and alien introduction?60 families, 247 species. Exotic species on the Island tere spontaneously introduced by local residents for many years. There were 178 ancient trees (17 species) survived on the Island. Meanwhile, the propose about protection of trees on Gulangyu Islet was put forward. References | Related Articles | Metrics

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Inhibition of Ginkgo Phenols on Five Vegetable Pathogenic Fungi LIN Qing-hong,LI Jin-yu,CHEN Fei,HUANG Yu-huan,LIN Guang-rong,LIN Zhi-ka,XU Wen-jiang 2014, 43(02): 152-155.  DOI: 10.3969/j.issn.1009-7791.2014.02.014 Abstract ( )   PDF (286KB) ( )   Ginkgo phenols exocarp refluxed with 70% ethanol extraction of active ingredients ginkgo phenols obtained with the crude extract with ether, 5% Na2CO3, 5% NaOH extraction , to obtain ginkgo phenols; In this study, using GC-MS identified the extract as ginkgo phenols. Bioassary to Ginkgo Phenols for the tested fungi showed that the Ginkgo Phenols was effective to Pellicularia rolfsii, in which the EC50 was 1.36 mg.mL-1. References | Related Articles | Metrics

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Field Efficacy Evaluation of Five Kinds of Chemicals on Solenopsis invicta ZHENG Xiu-fang 2014, 43(02): 156-158.  DOI: 10.3969/j.issn.1009-7791.2014.02.015 Abstract ( )   PDF (201KB) ( )   By means of the active mound decrease rate and worker ants decline rate indexes, the author evaluated five chemicals’ efficacy of preventing Solenopsis invicta, including ‘Solenopsis invicta Killer (IV-Type)’, ‘Mieyiwei’, ‘Huoyijing’, ‘Yijingan’ and ‘Mieyihuang’. The results show that there are some differences of the control efficacy between these chemicals. After 21 d treatment, ‘Solenopsis invicta Killer(IV-Type)’ and ‘Mieyiwei’ showed good control efficacy. The control efficacy of the nest prevention and worker ants can reach more than 90.0%. It is suggested that we should alternate these two chemicals to delay the drug-resistant of Solenopsis invicta. References | Related Articles | Metrics

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Tissue Culture Techniques of Blueberry (Britewell) TAO Jun-feng,LI Ye-fang,SONG Jie,GUAN Wen-ling 2014, 43(02): 159-163.  DOI: 10.3969/j.issn.1009-7791.2014.02.016 Abstract ( )   PDF (304KB) ( )   In order to obtain efficient tissue cultural propagation technology of blueberry, the semi-lignification shoots of blueberry (Britewell) used as explants, propagation medium, rooting culture medium was selected. The result showed that 1/2MS＋2.0 mg·L-1 ZT＋0.01 mg·L-1 NAA＋30 g·L-1 sugar＋6.5 g·L-1 agar＋pH 5.8 is the optimal propagation medium to blueberry (Britewell), has 87% rate of buds induction on aseptic seeding, and the coefficient multiplication is 3.6，Improved ZT concentration, more callus emerged in propagation medium. its contributed to improving multiplication but also caused more thin aseptic seeding; 1/2 MS＋1.5 mg·L-1 IBA＋0.01 mg·L-1 NAA＋25 g·L-1 sugar＋7 g·L-1 agar＋pH 5.8 is the optimal rooting medium, has 70% rooting percentage. Being acclimatized for 10 d, the rooted plantlets were transplanted to turfy-soil and the survival rate was 78%. Control callus produced and cheaper hormones applicated is the key of efficient tissue cultural propagation technology of blueberry. References | Related Articles | Metrics

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Tissue Culture of Precious Timber Species Toona ciliata Roem CHEN Li-wen,SHI Qun,LIANG Gang,CAI Lin,HE Gui-zheng 2014, 43(02): 164-167.  DOI: 10.3969/j.issn.1009-7791.2014.02.017 Abstract ( )   PDF (254KB) ( )   The seeds of Toona ciliata were used as explants to study the tissue culture of T. ciliata. The results showed that the MS＋6-BA 0.5 mg·L-1＋NAA 0.2 mg·L-1 media was good for the bud induction of the asepsis seedling, the optimal bud proliferation culture medium was 2/3MS＋6-BA 0.5 mg·L-1＋NAA 0.2 mg·L-1＋GA3 1.0 mg·L-1, and the optimal root induction culture medium was 1/2MS＋IBA 0.5 mg·L-1, The plantlet was transplanted with the matrix of the peat soil, yellow subsoil and river sand, and the survival rates up to 85%. References | Related Articles | Metrics

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Effects of PEG on Germination of Bauhinia Pollens WANG Xiao-liang 2014, 43(02): 168-169.  DOI: 10.3969/j.issn.1009-7791.2014.02.018 Abstract ( )   PDF (493KB) ( )   The effects of different concentration of PEG on Bauhinia (Cercis chinensis) pollen germination were studied in this paper. The results showed that 5～30 g·L-1 PEG promoted the Bauhinia pollen germination rate and tube length, the pollen germination rate and tube length in the treatment of 20 g·L-1 concentration were both significantly higher than the other treatments. References | Related Articles | Metrics

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Research Advance of Heme Oxygenase Physiological Function in Plant WANG Guang-hui,WU Huang-ming,ZHENG Yan,LI Cheng-liang,CHEN Hui-ping, 2014, 43(02): 170-176.  DOI: 10.3969/j.issn.1009-7791.2014.02.019 Abstract ( )   PDF (347KB) ( )   Heme oxygenase (HO) is a key enzyme that catalyzes the oxidative degradation of heme to carbon monoxide (CO), free iron, and biliverdin (BV)．This review focuses on the recent advances of HO research made in plant including HO playing the role in phytochrome synthesis，regulating the constitution in morphological architecture of root，raising the resistance of plant against stress，and promoting the germination of seeds and regulating the closure of stoma, etc. Forthmore，the direction of coming research is made a discussion． References | Related Articles | Metrics

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Application Progress of Near Infrared Spectroscopy in Determination of Rice Characteristics WU Wei-zheng,CHEN Hong-peng 2014, 43(02): 177-182.  DOI: 10.3969/j.issn.1009-7791.2014.02.020 Abstract ( )   PDF (270KB) ( )   As a new detection technology, near infrared spectroscopy is widely applied in many industries including agriculture, forestry, industry, medicine, food and others. This paper reviews the application progress of near infrared spectroscopy in determination of rice characteristics, including analysis of carbohydrates, proteins and lipids and other nutrients, analysis of starch pasting property, viscosity and palatability, analysis of nitrogen, phosphorus, potassium and other nutrient elements content in the process of rice growth, identification of varieties and breeding, analysis of disease and heavy metals and other aspects. However, some defects of the spectral technology in the determination of rice characteristics are briefly discussed as well as the prospects of the technology with the current development trend. References | Related Articles | Metrics

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A Review on Pharmacological Effects of Epimedium LIU Kai-jie,LI Chao 2014, 43(02): 183-186.  DOI: 10.3969/j.issn.1009-7791.2014.02.021 Abstract ( )   PDF (315KB) ( )   The pharmacological effects of epimedium in recent three years were summarized. And the pharmacological effects in reproductive system, bone, cardiovascular, cancer, immunity, memory and asthma, etc were generalized. It provided some references for the further research of epimedium . References | Related Articles | Metrics