Subtropical Plant Science ›› 2014, Vol. 43 ›› Issue (04): 276-280.DOI: Doi: 10.3969/j.issn.1009-7791.2014.04.002

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Effects of Five Kinds of Heavy Metal Stress on Seed Germination and Seedling Growth of Trifolium repens and T. pratense

SONG Feng-ming,LIU Jian-hua,WU Cai-qiong,LEI Jiang-li,XU Yi-yan   

  1. 1.Shenzhen Techand Ecology & Environment Company Limited in Guangdong Province, Shenzhen 518040, Guangdong China; 2.Fairylake Botanical Garden, Shenzhen & Chinese Academy of Sciences, Shenzhen 518004, Guangdong China
  • Received:2014-09-26 Revised:2015-01-26 Online:2014-12-10 Published:2014-12-10
  • Contact: XU Yi-yan

五种重金属胁迫对白三叶和红三叶种子萌发及幼苗生长的影响

宋凤鸣,刘建华,吴彩琼,雷江丽,徐义炎   

  1. 1.深圳市铁汉生态环境股份有限公司,广东 深圳518040;2.深圳市中国科学院 仙湖植物园,广东 深圳 518004
  • 通讯作者: 徐义炎
  • 作者简介:宋凤鸣,硕士,从事边坡绿化研究。
  • 基金资助:

    深圳市生物、互联网、新能源、新材料产业发展专项资金项目(SW201110069)

Abstract: Effects of five kinds of heavy metal stress on seed germination and seedling growth of Trifolium repens and T. pratense were studied by germination test, aimed to provide a reference for the study of the applications of Trifolium in heavy metal contaminated soil. The results showed that Haifa (a cultivar of T. repens)and T. pratense had better tolerance to Cd2+ and Mn2+, than to Pb2+, Cu2+ and Zn2+.They had over 80% germination rate under the III grade standard concentration of Cd2+ or under 800 mg稬-1 Mn2+, and the seedlings still maintained good vitality. High concentration of heavy metal ions stress had significant inhibition on the growth of embryo and hypocotyl, and T. pratense had better germination rate than Haifa. These two species of Trifolium could be applied for Cd-Mn contaminated soil restoration.

Key words: Trifolium, heavy metal ions, stress, seed germination

摘要: 通过发芽试验探讨镉、锰、铅、铜、锌五种重金属离子胁迫对海发白三叶和红三叶种子萌发的影响。结果表明:海发白三叶和红三叶较耐镉和锰,铅、铜和锌次之。在国家土壤三级标准的镉离子浓度和800 mg·L-1锰离子浓度胁迫下仍有80%以上的发芽率,且幼苗均能保持较好活性。高浓度重金属离子对两种三叶草胚轴和胚根生长有显著抑制,红三叶发芽率整体略优于海发白三叶;两种三叶草均适合于镉、锰污染的土壤修复。

关键词: 三叶草, 重金属离子, 胁迫, 种子萌发

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