Abstract: The tissue culture techniques of four cultivars of Dracaena fragrans used stem section as the explant were analyzed. A series of important conclusions can be made: 1) The optimal medium for controlling tissue browning of D. fragrans: MS + 0.25 g·L-1 VC + 0.50 g·L-1 Na2S2O3; 2) The optimal medium for inducing the D. fragrans stem stirring: MS+ 3.0 mg·L-1 6-BA + 0.2 mg·L-1 NAA + 0.1 mg·L-1 KT; 3) The optimal proliferation medium of D. fragrans, depends on the cultivars of D. fragrans. The optimal medium for D. fragrans and D. fragrans ‘Massangeana’: MS + 2.0 m g·L-1 6-BA + 1.0 mg·L-1 NAA + 0.1 mg·L-1 GA3; The optimal medium for D. fragrans ‘Compacta’ and D. fragrans ‘Janet-craig’: MS + 1.0 m g·L-1 6-BA + 0.5 mg·L-1 NAA + 0.1 mg·L-1 GA3; 4) Under the condition of MS + 0.05 mg·L-1 6-BA + 0.05 mg·L-1 NAA + l g·L-1 AC was the most efficient the Dracaena fragrans seedling medium; 5) 1/2MS + 0.5 mg·L-1 IBA was the most efficient rooting medium.

Key words: Dracaena fragrans, tissue culture, callus browning

摘要： 以4品种也门铁的茎段为外植体进行组织培养技术研究。结果表明，控制普通也门铁茎段褐化效果最理想的培养基为MS + 0.25 g·L-1 VC + 0.50 g·L-1 Na2S2O3；诱导也门铁不定芽萌动的最佳培养基为MS + 3.0 mg·L-1 6-BA + 0.2 mg·L-1 NAA + 0.1 mg·L-1 KT；诱导也门铁不定芽增殖的最佳培养基，因品种不同而异，普通也门铁和金心也门铁为MS + 2.0 mg·L-1 6-BA + 1.0 mg·L-1 NAA + 0.1 mg·L-1 GA3，扭纹铁和金心扭纹铁为MS + 1.0 mg·L-1 6-BA + 0.5 mg·L-1 NAA + 0.1 mg·L-1 GA3；也门铁壮苗的最佳培养基为MS + 0.05 mg·L-1 6-BA + 0.05 mg·L-1 NAA + l g·L-1AC；也门铁生根最优培养基为1/2MS + 0.5 mg·L-1 IBA。

关键词: 也门铁, 组织培养, 褐化