Archive

10 March 2015, Volume 44 Issue 01 Previous Issue    Next Issue
---
Plant physiology, biochemistry and molecular biology



For Selected:

Download Citations EndNote Ris BibTeX

Toggle Thumbnails

Select

Genetic Variation Analysis of Salt-tolerant Mutant from Maize Inbred Lines (AS-9) YANG Feng-jiao,LI Hong-ying,LU Cun-fu,CHEN Yu-zhen 2015, 44(01): 1-7.  DOI: 10.3969/j.issn.1009-7791.2015.01.001 Abstract ( )   PDF (486KB) ( )   Chemical mutation was an effective way of creating new germplasm resources. To analyze the impact of chemical mutagenesis on AS-9 maize inbred offspring, the salt tolerance and genetic variation between mutant M4 and the corresponding original material (AS-9) were evaluated by using 44 pairs of SSR markers that may be associated with root traits. Seed germination, bud length and root number of M4 were higher than that of AS-9. After treatment with 200 and 250 mmol.L-1 NaCl for 7 days, root numbers of IV400 and IV1600 seedlings were higher than that of AS-9, with increases of 23.1% and 19.1%, respectively.IV1600 primary root lengths of 1, 2, 3 were 1.33, 1.70, and 2.15 times longer than that of AS-9, respectively. Twenty five pairs of SSR primers gave profiles amplified in sample of M4 mutants, accounting for 56.82% of the total pairs of primers. The average value of genetic similarity coefficient between IVCK and M4 was 0.6563. With the centered genetic similarity coefficient of -0.04 by UPGMA, the maize inbred line materials were divided into three groups: the original material (IVCK), the M4 mutants including IV1400, IV1600, IV2000, and other M4 mutants (IV200, IV400, IV800, IV1200). Principal component analysis and clustering results were similar. In addition, SSR-PCR products amplified by six pairs of primers were sequenced, the results showed that there was distinct variation between AS-9 and the induced M4, indicating chemical mutagenesis made maize inbred AS-9 produce a wide range of genetic variation. References | Related Articles | Metrics

Select

Analysis of GC-MS on Fruit Aroma Components of Different Strawberry Cultivars ZENG Xiang-guo,HAN Yong-chao,XIANG Fa-yun,YANG Yan-fang,CHEN Feng-ying,GU Yu-cheng 2015, 44(01): 8-12.  DOI: 10.3969/j.issn.1009-7791.2015.01.002 Abstract ( )   PDF (296KB) ( )   The head space-solid phase microextraction and gas chromatography- mass spectrometry were applied to analyze aroma composition of five strawberry cultivars (Sweet Charile, Jingyao, Jingyu, Akihime, Toyonoka). The results indicated 59.36%—77.12% esters components exited in volatile components of five cultivars. Ethyl butanoate, ethyl hexanoate, linalool, furanone, methyl butanoate, methyl hexanoate, 2-heptanone and nerolidol were the most important aroma compounds in fruit of five cultivars. References | Related Articles | Metrics

Select

Extraction and Composition of Polysaccharide from the Fruits of Campnumoea lancifolia LI Bin,LI Gui,CHEN Gong-xi,LI Hui,LIANG Jun-peng,CHEN Ya 2015, 44(01): 13-17.  DOI: 10.3969/j.issn.1009-7791.2015.01.003 Abstract ( )   PDF (354KB) ( )   Polysaccharides (PS) from the fruits of Campnumoea lancifolia were extracted and purified by ethanol precipitation, ethyl acetate and sevage reagent with ultrasonic assistance, and the content was determined by the phenol-sulfuric acid method. The components of the polysaccharides were identified by gas chromatography-mass spectrometry (GC-MS). The results showed that the average content of polysaccharide in the fruits of C. lancifolia reached up to 45.80%, and main monosaccharide composition and relative contents were arabia sugar (5.00%), xylose (9.65%), mannose (11.55%), fructose (30.20%), galactose (33.85%). Related Articles | Metrics

Select

Photosynthetic Physiological Analysis on Dwarf Multi-Tillering Rice Mutant CA648 LIU Xiao-peng,ZHU Yun-hua,LIU Qing,SHI Qi,LI He-song 2015, 44(01): 18-22.  DOI: 10.3969/j.issn.1009-7791.2015.01.004 Abstract ( )   PDF (302KB) ( )   The chlorophyll content, photosynthetic rate, sugar and sucrose contents, POD enzyme activity of rice mutant (CA648, performing a dwarf and multi-tillering phenotype) and wild type ZH11 were preliminary studied. The results showed that the chlorophyll content of ZH11 was higher than that of CA648 at tillering stage. At the early stage of tillering, the net photosynthetic rate, stomatal conductance, and transpiration rate of CA648 were lower than those of ZH11, but intercellular CO2 concentration in CA648 was higher than that in ZH11. At the top tillering stage (20 d after tillering stage), the significant difference of the net photosynthetic rates of two kinds of material was found. The net photosynthetic rate of ZH11 was about 17.5% higher than CA648. Meanwhile, stomatal conductance, transpiration rate and intercellular CO2 concentration of ZH11 were also higher than those of CA648. At the late tillering stage, the net photosynthetic rates of ZH11 and CA648 were almost the same. At the top tillering stage, reducing sugar content in CA648 leaf was significantly higher than the control, while there was no difference of reducing sugar content in the two kinds of stems. The sucrose content of leaf in CA648 was lower than the control, about 77.5% of ZH11, while the sucrose content in the stem was 30.7% higher than ZH11. There were obvious differences of POD enzyme activity between two kinds of material. At the mid-tillering stage, the POD activities in leaf and stem of CA648 were 73.0% and 16.8% higher than those in ZH11, respectively. References | Related Articles | Metrics

Plant physiology, biochemistry and molecular biology

Select

Tissue Culture Technique of Dracaena fragrans QI An-min,WANG Jia-qiong,LI Nan,HE Tao,LI Wei,CUI Da-fang 2015, 44(01): 23-28.  DOI: 10.3969/j.issn.1009-7791.2015.01.005 Abstract ( )   PDF (328KB) ( )   The tissue culture techniques of four cultivars of Dracaena fragrans used stem section as the explant were analyzed. A series of important conclusions can be made: 1) The optimal medium for controlling tissue browning of D. fragrans: MS + 0.25 g·L-1 VC + 0.50 g·L-1 Na2S2O3; 2) The optimal medium for inducing the D. fragrans stem stirring: MS+ 3.0 mg·L-1 6-BA + 0.2 mg·L-1 NAA + 0.1 mg·L-1 KT; 3) The optimal proliferation medium of D. fragrans, depends on the cultivars of D. fragrans. The optimal medium for D. fragrans and D. fragrans ‘Massangeana’: MS + 2.0 m g·L-1 6-BA + 1.0 mg·L-1 NAA + 0.1 mg·L-1 GA3; The optimal medium for D. fragrans ‘Compacta’ and D. fragrans ‘Janet-craig’: MS + 1.0 m g·L-1 6-BA + 0.5 mg·L-1 NAA + 0.1 mg·L-1 GA3; 4) Under the condition of MS + 0.05 mg·L-1 6-BA + 0.05 mg·L-1 NAA + l g·L-1 AC was the most efficient the Dracaena fragrans seedling medium; 5) 1/2MS + 0.5 mg·L-1 IBA was the most efficient rooting medium. References | Related Articles | Metrics

Select

Allelopathy of Water Extracts from Root of Mirabilis himalaica on Five Speices of Plants MA Shi-yao,LIU Sheng-li,CHEN Yu-zhen,LU Cun-fu,LAN Xiao-zhong 2015, 44(01): 29-35.  DOI: 10.3969/j.issn.1009-7791.2015.01.006 Abstract ( )   PDF (349KB) ( )   To investigate the allelopathic effects of Mirabilis himalaica root,which was picked from Tibet Nyingchi region, on crop and vegetable plants, different concentrations (0.0125, 0.025, 0.05, 0.1 g·mL-1) of water extracts were used as treatment solutions to asses their effects on seed germination and seedling growth of five plant species (wheat, mung beans, lettuce, rape, and radish). The results showed that with the increase of the concentration of aqueous extract, the seed germination and seedling growth of the five plants were inhibited. Accordingly, antioxidant enzyme activity and chlorophyll content decreased, while the content of malondialdehyde was significantly increased. Allelopathic effects indicated that the underground part was more sensitive to the M. himalaica root water extracts than the aboveground part of the five plants. The results also conformed that allelopathic effects were species-specific and concentration- dependent. The sensitivity order was as follows: lettuce> mung beans>wheat> radish> rape. References | Related Articles | Metrics

Select

Floristic Characteristics of Seed Plants in Yihuang Mergus squamatus Provincial Nature Reserve from Jiangxi YE Jing,MA Jian-bo,LI Yun-jing,YU Dao-ping,LIU Yi-zhen, 2015, 44(01): 36-41.  DOI: 10.3969/j.issn.1009-7791.2015.01.007 Abstract ( )   PDF (297KB) ( )   The flora of Yihuang Mergus squamatus provincial nature reserve were surveyed and analyzed. The results showed that: (1) 776 species of spermatophytes affiliated to 428 genera in 119 families were discovered in the nature reserve, the families of flora were dominated by Gramineae (47 genera/80 species, similarly hereinafter), Compositae (35/51), Rosaceae (13/37) etc., the genera of flora were dominated by Polygonum and Rubus. (2) There was a high proportion in genera and families which contain one to four species, indicated the differentiation of the flora in this nature reserve. (3) The distribution types of families could be classified into 8 distribution types and 7 subtypes, and the ratio of tropical geographical elements to temperate geographical elements of families were 2.43, showed a tightness relation with tropical flora of the families in this nature reserve. Furthermore, the high proportion of cosmopolitan elements in families implied the intrazonal feature of the flora. (4) The distribution types of genera could be classified into 14 distribution types and 15 subtypes, and the ratio between tropical geographical elements and temperate geographical elements of genera were 1.05, suggested that the flora transitional character from tropical flora to temperate flora. The floristic characteristics show that the nature reserve provides an advantageous habitat for Mergus squamatus to winter, which is an extremely endangered bird affirmed by BirdLife International. References | Related Articles | Metrics

Select

Natural Regeneration of Rhododendron moulmainense Forest in Wutong Mountain of Shenzhen LIU Yong-jin,CHEN Shi-qing,YUAN Yin,SUN Li-fang 2015, 44(01): 42-46.  DOI: 10.3969/j.issn.1009-7791.2015.01.008 Abstract ( )   PDF (310KB) ( )   The regeneration characteristics of Rhododendron moulmainense forest were investigated by sample plot method. It was showed that there were 12 species with larger amount of seedlings in the understory. The results showed that the population of Machilus chekiangensis, Ilex viridis had great regeneration ability; the population of Itea chinensis, Machilus breviflora, Rapanea neriifolia, Lindera communis had medium regeneration ability; the population of Litsea rotundifolia var. oblongifolia, Schefflera octophylla, Symplocos lancifolia, Rhododendron moulmainense, Enkianthus quinqueflorus Ficus hirta had bad regeneration ability. The seedlings of Rhododendron moulmainense had only been found in two sample plots among the six sample plots, and no seedlings lower than 30 cm. Besides, Rhododendron moulmainense forest had low forest density. So the natural regeneration of Rhododendron moulmainense cannot keep its landscape sustainable. References | Related Articles | Metrics

Select

The Investigation of Growth Characteristics of Eucalyptus dunnii in Different Site Type in Northern Fujian LIU Jian-bin 2015, 44(01): 47-51.  DOI: 10.3969/j.issn.1009-7791.2015.01.009 Abstract ( )   PDF (295KB) ( )   This study investigated diameter at breast height (DBH) and height of nine years old Eucalyptus dunnii in different site type. The average DBH and height of E. dunnii in mountain forest 1 were 11.6 cm and 11.5 m, the average DBH and height of E. dunnii in mountain forest 2 were 13.0 cm and 13.1 m, the average DBH and height of E. dunnii in riparian forest were 21.3 cm and 17.0 m. The indexs of fast-growing and high yield of E. dunnii in different type lands were higher than local native species, such as masson pine and fir tree, though the growth of E. dunnii in different type lands was obviously different. The differences of indexes due to high growth differentiation of E. dunnii, showed that adaptability of E. dunnii in introduction area was strong. References | Related Articles | Metrics

Select

Effects of Different Cultivation Techniques on Production of Bud Sticks of Cinnamomum camphora var. linaloolifera ZHANG Yao-xiong 2015, 44(01): 52-55.  DOI: 10.3969/j.issn.1009-7791.2015.01.010 Abstract ( )   PDF (296KB) ( )   The effects of different afforestation measures, such as fertilization, cutting trunk measures and time of cutting trunk on bud sticks of Cinnamomum camphora var. linaloolifera, have been studied. The results show different seasons of fertilization, different cutting trunk measures and different times of cutting trunk have different effects on yield and quality of bud sticks. Using base fertilization in March and add quick fertilization in August again can significant increasing output and quality of cuttings; cutting trunk measures do surely with the position of 20—30 cm can promote obviously quality of cuttings; cutting trunk in July or early March, cuttings of quality and quantity is most considerable. References | Related Articles | Metrics

Select

Additions to Fern Flora in Fujian Province, China LIN Qin-wen 2015, 44(01): 56-57.  DOI: 10.3969/j.issn.1009-7791.2015.01.011 Abstract ( )   PDF (331KB) ( )   The two new recorded species of fern in Fujian were Lepisorus miyoshianus (Makino) Fraser-Jenkins & Subh. and Elaphoglossum marginatum燭. Moore. The voucher specimens were preserved in the herbarium of Fujian Guilongshan Provincial Nature Reserve (FGNR) References | Related Articles | Metrics

Select

Ornamental Plants and Their Landscape Application in Hong Kong XIE Yuan-tao,DAI Se-ping 2015, 44(01): 58-64.  DOI: 10.3969/j.issn.1009-7791.2015.01.012 Abstract ( )   PDF (359KB) ( )   The ornamental plant resources and their landscape applications were investigated from some urban streets, parks in Hong Kong on the basis of field survey and data collection. There are 260 species of 70 families of ornamental plant catalogued, and the characters of ornamental plants and landscape application were discussed and analyzed. Problems and suggestions were proposed on exploring the ornamental plant resources to promote the development of landscape in Hong Kong. References | Related Articles | Metrics

Select

Vegetation Survey and Landscape Pattern Analysis of Donghu Park in Jieyang, Guangdong Province TANG Hai-rong,YU Xi-hong,ZHENG Bin-jie,LIN Li-jia 2015, 44(01): 65-69.  DOI: 10.3969/j.issn.1009-7791.2015.01.013 Abstract ( )   PDF (329KB) ( )   Based on the landscape ecological theory, vegetation survey of Donghu Park was conducted. Meanwhile, several landscape pattern indexes such as diversity, patch area ratio, dominance, evenness were selected to analyze the landscape pattern of the park. The results showed that the vegetation of Donghu Park was rich, landscape pattern was generally rational, and the structure of landscape was stable in general. But there was a need to improve vegetation configuration, especially strengthen the seasonal aspect variation, and increase shrub and grassland patches to enrich landscape structure. References | Related Articles | Metrics

Select

Tissue Culture of Murraya tetramera LIN Qian,GAO Ying-ying,HAN Xiao-hua,ZHANG Lu,HUANG Tian-kun,LIN Gui-mei 2015, 44(01): 70-71.  DOI: 10.3969/j.issn.1009-7791.2015.01.014 Abstract ( )   PDF (233KB) ( )   Stems with newborn axillary bud used as explant, sampling times, concentrations of 6-BA, type of auxins were researched as important factor affecting growth of explants. Also, the effect of activated carbon on browning of explants was studied. Appropriate sampling time for tissue culture of Murraya tetramera was April, appropriate 6-BA concentration for the shoots induction from explants was 2.5 mg·L-1, appropriate auxin was IBA and 1.5 g·L-1 activated carbon could reduce the browning of explants. The results showed that the initial media was MS + 6-BA 2.5 mg·L-1 + IBA 0.1 mg·L-1 + GA3 0.3 mg·L-1 + AC 1.5 g·L-1 in tissue culture of M. tetramera. References | Related Articles | Metrics

Select

Research Advances in Mycoviruses of Botrytis LI Wen-jing,WU Ming-de,LI Guo-qing 2015, 44(01): 72-76.  DOI: 10.3969/j.issn.1009-7791.2015.01.015 Abstract ( )   PDF (259KB) ( )   This article reviewed the species of mycovirus infecting Botrytis spp. and their effects on the host fungi. In addition, the morphology and genomic structure of five species of Botrytis viruses were described. Based on the current research situation of Botrytis mycoviruses, the direction for the further research were proposed, which provides reference for the study of Botrytis mycoviruses and using mycoviruses for biocontrol. References | Related Articles | Metrics

Select

Advances on Chemical Constituents and Pharmacological Activities of Scutellaria barbata LIN Jing-yi,LIU Shao-song,MING Yan-lin 2015, 44(01): 77-82.  DOI: 10.3969/j.issn.1009-7791.2015.01.016 Abstract ( )   PDF (331KB) ( )   Scutellaria barbata, known as Banzhilian in traditional Chinese medicine, has multifunction such as clearing heat, dispersing blood stasis, analgesia and diuretic. Phytochemistry research reveals S. barbata contains flavonoids, diterpanes, essential oil and polysaccharides, etc. Pharmacological research shows that S. barbata displays antitumor, antioxidant, antibacterial, immunoregulatory activity. This review summarizes the progress on chemical constituents and pharmacological activities of S. barbata, which provides reference for further development and utilization of S. barbata. References | Related Articles | Metrics

Select

Review of Sexual Reproduction Characteristics of Amomum villosum LIN Mei-zhen,TIAN Hui-qiao 2015, 44(01): 83-86.  DOI: 10.3969/j.issn.1009-7791.2015.01.017 Abstract ( )   PDF (358KB) ( )   Amomum villosum is rare and endangered medicinal plants in China, its fruit is used as medicine. Natural pollination seed setting rate is low for stigma being higher than anther, which severely limits its fruit production. In order to reveal the basis of forming fruit and explore corresponding strategy of improving the yield of A. villosum, the paper summarized research status on sexual reproduction such as morphological characteristics, ecological characteristics, pollen development, embryo sac development, flowering, pollination and fertilization. References | Related Articles | Metrics

Select

Analysis of the Contradictions of Keys and Its Significance in Practice WU Zeng-hua,HOU Xue-liang 2015, 44(01): 87-90.  DOI: 10.3969/j.issn.1009-7791.2015.01.018 Abstract ( )   PDF (191KB) ( )   Based on analysis of the structure of keys, this paper deems that the fundamental contradiction of keys is the contradiction between the individual characters of specimens and the common characters used in keys. The principal contradiction of single-access key is the contradiction between the characters used conditionally in keys and the finite characters of individual specimens, so single-access key cannot well solve the fundamental contradiction and is limited in applicative areas and on using effect. The principal contradiction of multi-access key is a contradiction between the limited characters of character database and the unlimited individual characters of species, hence there is not the contradiction between individual characters and the common characters. Therefore, multi-access key well resolves the fundamental contradiction and can be utilized expediently. References | Related Articles | Metrics