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30 December 2018, Volume 47 Issue 04 Previous Issue    Next Issue
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Plant physiology, biochemistry and molecular biology
Plant ecology, resources and taxonomy
Plant cultivation and tissue culture
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Plant physiology, biochemistry and molecular biology

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Expression of PP2C Related Genes in Dormancy Process of Pyrus pyrifolia WANG Hui-quan, LI Liang, YU Zhi-xiong, YUAN Ya-fang, WU Shao-hua 2018, 47(04): 305-311.  DOI: 10.3969/j.issn.1009-7791.2018.04.001 Abstract ( )   PDF (560KB) ( )   Plant Protein Phosphatase 2C (PP2C), as a widespread protein phosphatase in plants, plays an important role in plant signal transduction pathway. Expression of PP2C related genes in dormancy process of Pyrus pyrifolia was analyzed. PP2C-related genes of P. pyrifolia were highly homologous to PP2C genes of Prunus. Different PP2C genes played different roles in dormancy of pear bud. PP2C-37-1、PP2C-37-2、PP2C-51-1 and PP2C-24 might regulate endodormancy, PP2C-78 gene might has a significant regulatory effect on release of endodormancy. ABA signal transduction complex was formed by ABA receptor PYR/PYL protein, PP2C protein and SnRK2 (protein kinase) protein, and then acted on transcription factor ABF, which resulted in regulating dormancy of pear flower bud. References | Related Articles | Metrics

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Chlorophyll Content and Photosynthetic Efficiency of Six Thalloid Bryophytes ZHANG Wei, GAO Xue-di, DING Xiao-lu, SUN Li-wei, ZHUANG Li-jie, WANG Jing-ting, PENG Tao, LIU Xing-xing, TANG Lu-yan 2018, 47(04): 312-316.  DOI: 10.3969/j.issn.1009-7791.2018.04.002 Abstract ( )   PDF (731KB) ( )   Using six thalloid bryophytes as materials, the corresponding relationship between chlorophyll content and actual photosynthetic quantum yield was determined. Results showed that the median value of total chlorophyll content of six species was 6.06 mg·g-1, with the highest value of 9.72 mg·g-1 in Pellia endiviifolia and the lowest of 4.46 mg·g-1 in Marchantia emarginata; the ratio of chlorophyll a/b ranged from 1.61 to 2.04, with the highest value in Phaeoceros carolinianus and the lowest in Dumortiera hirsuta; the actual photosynthetic quantum yields of three species, i.e. Plagiochasma rupestre, Marchantia emarginata and Conocephalum conicum, were significantly higher than those of Dumortiera hirsuta, Pellia endiviifolia and Phaeoceros carolinianus. This study indicated that there was no simple linear relationship between chlorophyll content and actual photosynthetic quantum yield in thalloid bryophytes. Habitats and air chamber of gametophytes may affect their actual photosynthetic quantum yield, which further enriches the ecological adaptation theory of bryophytes to light. References | Related Articles | Metrics

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Extraction Technology of Chlorogenic Acid with Low Caffeine in Coffee Beans CHEN Wei, LI Ni, HUANG Wen, CHEN Liang-hua, LIN He-tong, MING Yan-lin 2018, 47(04): 317-321.  DOI: 10.3969/j.issn.1009-7791.2018.04.003 Abstract ( )   PDF (379KB) ( )   The method of high performance liquid chromatography with dual wavelength was established for simultaneous determination of caffeine and chlorogenic acid in coffee bean extracts. The result showed a good linear relationship between peak area and concentration in the range of 0.2—1.0 mg·mL-1. The regression equations of caffeine were: Y=44077X+227.78, R=0.9997, RSD=0.78%; the regression equations of chlorogenic acid were: Y=34896X+289.96, R=0.9995, RSD=0.93%. With chlorogenic acid content as indicator, the optimal conditions for extracting chlorogenic acid from green coffee beans were determined as follows: ethanol concentration was 20%, solid-liquid ratio was 6:1, extraction time was 60 min, the extraction temperature was 70 ℃ according to orthogonal test and single factor test. Taking caffeine and chlorogenic acid contents as indicators, the best decaffeination extractant was cyclohexane-trichloromethane-acetone mixture, and the extraction process was extracted three times with 1.5 volumes of cyclohexane-trichloromethane- acetone mixture with the caffeine content decreased from (3.52±0.08)% to (0.47±0.11)%. References | Related Articles | Metrics

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Determination and Analysis of Amino Acids in Pulp of Different Citrus Cultivars ZHANG Di, XIE Hong-gen, PAN He-li, SUN Jian-hua, CHEN Yuan, PAN Dong-ming 2018, 47(04): 322-326.  DOI: 10.3969/j.issn.1009-7791.2018.04.004 Abstract ( )   PDF (327KB) ( )   The amino acid content of citrus pulps were determined by Hitachi L-8800 amino acid analyzer.The results showed that the pulp of citrus fruit was rich in aspartic acid, proline and glutamate. The total amino acid content of umbilical cord orange 52 pulp (7.04 g·100g-1) was higher, and the lowest was red flesh pomelo (4.53 g·100g-1), 1.5 times higher than that of the latter. Aspartic acid (Asp) was the most abundant amino acid in 17 of the 5 varieties. The trend of TAA and EAA content in all varieties was basically the same, that is, the higher the TAA content was, the higher the EAA content was. The order of the contents of various taste amino acids was not completely consistent among the varieties, which indicated that there were significant differences in nutrition between different citrus varieties. References | Related Articles | Metrics

Plant ecology, resources and taxonomy

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Indigenous Isolates of Arbuscular Mycorrhizal Fungi in Citrus Orchard Soil and Their Growth-Promoting Effects WU Wei, ZHOU Yang, CHEN Jie-zhong, ZHU Hong-hui, YAO Qing 2018, 47(04): 327-332.  DOI: 10.3969/j.issn.1009-7791.2018.04.005 Abstract ( )   PDF (509KB) ( )   Citrus plants are highly dependent on arbuscular mycorrhizal (AM) fungi, therefore, it is of practical significance to screen efficient isolates from citrus orchard soil. This study used 4 indigenous AMF isolates from citrus (‘Hongjiangcheng’ cultivar with Citrus haniana stock) orchard soil in Zengcheng, Guangdong Province as materials, investigated the growth-promoting effects of indigenous isolates on citrus plants. Results indicated that 4 isolates belonged to Scutellospora and Glomus, with root colonization rate of 12.7%—29.3%. Compared with the non-inoculated control, 4 indigenous isolates promoted the plant height, biomass and N, P, K nutrient content of ‘Hongjiangcheng’ seedlings, with mycorrhizal dependency of 9.4%—37.1%. Principle component analysis suggested that the growth-promoting effect of indigenous isolate ZCSP-D was similar to that of the commonly used efficient isolate Rhizophagus irregularis. References | Related Articles | Metrics

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Altitude Adaptability of Four Blueberry Cultivars in Guizhou WANG Di, ZHOU Ying, NIE Fei 2018, 47(04): 333-338.  DOI: 10.3969/j.issn.1009-7791.2018.04.006 Abstract ( )   PDF (370KB) ( )   Four cultivars of blueberry, i.e. Vaccinium ashei ‘Britewell’, V. ashei ‘Gardenblue’, V. corymbosum ‘Bluerain’, and V. corymbosum ‘Legacy’, were grown in different altitude regions, i.e. low altitude (800 m), mediate altitude (1100 m) and high altitude (2000 m) in Guizhou. And their relevant physiological indicators of leaves and fruits were determined. The results suggested that, physiologically, the MDA content and CAT activity of the four varieties showed a significant downward trend with increasing altitude, while the leaves’ REL, POD, SOD, and TC increased significantly with increasing altitude. When given a same elevation, the REL and MDA content of ‘Britewell’ leaves were the lowest among the four species, while its SOD activity appeared to be the highest in the three elevation zones. In terms of the quality of blueberry fruit, the VC content of ‘Legacy’ grown in high-altitude area performed the best, while that of ‘Britewell’ in high-altitude area ranked the lowest. As for low and mid-altitude areas, ‘Gardenblue’ had the highest VC content, and ‘Bluerain’ the lowest. The rank of anthocyanin content was as such: ‘Gardenblue’> ‘Britewell’> ‘Legacy’>‘Bluerain’; as for the average weight of blueberry fruit, the order was ‘Legacy’> ‘Britewell’ > ‘Gardenblue’ > ‘Bluerain’, which showed certain regularity; in low and mid-altitude areas, ‘Gardenblue’ produced the highest sugar-acid ratio out of the four varieties. And in high-altitude region, sugar-acid ratio of ‘Britewell’ appeared to be the highest. The above studies presented the resistance indexes and chlorophyll changes of the four blueberry varieties in regions of different altitudes in Guizhou, indicating that they were all equipped with certain stress resistance, that was, the adaptability against harsh environment, wherein, ‘Britewell’ was believed to had the best adaptability, with larger fruit, highest sugar-acid ratio in high altitude region, and the content of OPC as well as VC only following that of ‘Gardenblue’; the adaptability of ‘Britewell’ and ‘Legacy’ beats that of ‘Gardenblue’, whose fruit was smaller, with sugar-acid ratio ranking the highest in low and mid-altitude, the best palatability, and the highest VC and OPC content. References | Related Articles | Metrics

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Bryophyte Species Diversity of Alsophila Community in Chishui Alsophila National Nature Reserve WENG Tao, HUANG Wen-hu, HE Qin-qin, LIU Bang-you, ZHANG Ting-yue, LIANG Sheng, PENG Tao, YANG Shu-lin, TANG Lu-yan 2018, 47(04): 339-344.  DOI: 10.3969/j.issn.1009-7791.2018.04.007 Abstract ( )   PDF (407KB) ( )   Based on 165 bryophyte samples collected from 18 Alsophila plant quadrats of Chishui Alsophila Nature Reserve, 61 species belonging to 37 genera in 26 families were identified. Among these species, 16 species of 10 genera in 8 families were liverworts, 45 species of 27 genera in 18 families are mosses. Dominant families included Lophocoleaceae, Leucobryaceae, Fissidentaceae, Pylaisiadelphaceae and Hypnaceae. Dominant genera included Leucobryum, Ctenidium, Campylopus, Fissidens, Heteroscyphus. The quadrats with rich bryophyte species were located in moist valleys, in addition, bryophytes distributing more frequently in the environments where there were few small plants such as herbs, ferns and shrubs, few seedlings of Alsophila, or thick humus. Epilithic bryophytes were most abundant and accounted for 85.25% of all species. References | Related Articles | Metrics

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The Diversity of Coastal Ornamental Plants and Their Landscape Application in Fujian CHEN Heng-bin 2018, 47(04): 345-351.  DOI: 10.3969/j.issn.1009-7791.2018.04.008 Abstract ( )   PDF (376KB) ( )   This paper reports 111 taxa of coastal ornamental plants belonging to 51 families in Fujian, including 40 trees, 35 shrubs, 28 herbs and 8 vines. The landscape applications of these species, especially 10 species with high ornamental value and high application value were introduced. References | Related Articles | Metrics

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Moisture-tolerant Herbaceous Plants in Guangzhou’s Parks and Their Landscape Application XU Ze-hao, XIE Xiao-liang, XIAN Li-hua, WENG Shu-fei 2018, 47(04): 352-356.  DOI: 10.3969/j.issn.1009-7791.2018.04.009 Abstract ( )   PDF (1094KB) ( )   Six parks with good plant maintenance and management in Guangzhou were investigated. There were long natural gentle slope revetments in the parks. The article investigated the moisture-tolerant herbaceous plants of the parks, summarized their landscape application, then analyzed their landscape characteristics and existing problems. The results showed that, within investigation area, a total of 77 moisture-tolerant herbs belonging to 52 genera in 26 families were recorded, 26 of which were annuals, 50 of which were perennials, and left was fern. The mainly common moisture-tolerant herbs in Guangzhou’s parks were the low and middle herbs. The characteristics of moisture-tolerant herbs landscape application to Guangzhou’s parks as follows: ① Reconciling the color of plants, showed the water scenery; ② Using the varied lines to make the wetland more interesting; ③ Right place, right grass, reflecting the tropical characteristics; ④ Planting naturally and creating a view effect. Appropriate increase in native perennial moisture-tolerant herbs species should be taken into consideration in the construction of Guangzhou’s parks, and the popular science about the moisture-tolerant herbs should be strengthened. References | Related Articles | Metrics

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Landscape Quality Evaluation of Rhododendron moulmainense Aesthetic Forest in Shenzhen Wutong Mountain SHI Bo, WANG Ding-yue, CHEN Shi-qing, LI Wen-hua, LIN Jin-tao 2018, 47(04): 357-362.  DOI: 10.3969/j.issn.1009-7791.2018.04.010 Abstract ( )   PDF (363KB) ( )   In order to get a more accurate evaluation of the quality about Rhododendron moulmainense aesthetic forest, and provide a more comprehensive and general quality evaluation system about flowering tree of ornaments, on the basis of literature about evaluation of aesthetic forest, using analytic hierarchy process (AHP) to build the evaluation system and ascertain various levels index weights by 3 projects, 13 indexes and 38 classes. The index weight of the forest outside the landscape, the single tree landscape, and the forest inside the landscape are respectively 0.6483, 0.2270, 0.1591. Through field survey and evaluation, the flower screen score is 52.8466, with a better grade. The valley of R. moulmainense score is 44.5834, with a good grade, the double phoenix pavilion score is 20.0336, with a poor grade. References | Related Articles | Metrics

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Phylogenetic Relationship of Allium sect. Bromatorrhiza Inferred from Morphological Characters HU Chun-ya, JIANG Xiao-jun, WANG Yue-feng, CHEN Xu, HUANG De-qing 2018, 47(04): 363-369.  DOI: 10.3969/j.issn.1009-7791.2018.04.011 Abstract ( )   PDF (488KB) ( )   Allium section Bromatorrhiza is endemic to the Hengduan Mountain Region and neighboring areas. Its taxonomic circumscription and systematic position have been clarified by previous systematic works, but the infra-sectional taxonomic classification and interspecific relationships within the section are as yet unclear. Here, section Bromatorrhiza is cladistically analyzed based on 36 morphological characters using subgenus Amerallium species of circle-Mediterranean Region and North America as outgroups. The topology of the tree obtained is consistent with the results derived from molecular data. Sect. Bromatorrhiza and subg. Amerallium species of circle-Mediterranean Region form a good clade, suggesting more closely relationships between them. Within the section, its species are arranged into two obvious clades. One consists of A. fasciculatum (x = 10, two ovules) and all x = 11 species (one ovule), and the other clade (x = 7, two ovules) includes A. macranthum and A. wallichii. The results suggest that subsection Bromatorrhiza previously presented is artificial and should be cancelled. Combining the existed results, the present study suggests that sect. Bromatorrhiza comprises four independent evolutionary clades, viz. A. wallichii and its variety, A. macranthum, A. fasciculatum and all x = 11 species (including five species and one variety). Although subsection Uniovulatum formerly proposed is reasonable, we argue that a subdivision of section Bromatorrhiza seems unnecessary for such a relatively small number of species. References | Related Articles | Metrics

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New Records of Seed Plants from Jiangxi Province ZHANG Xin-jian, FENG Lu, SONG Han-zhang, YE Fan, FAN Qiang, LIAO Wen-bo 2018, 47(04): 370-376.  DOI: 10.3969/j.issn.1009-7791.2018.04.012 Abstract ( )   PDF (8160KB) ( )   Three genera and six species were reported as new records to Jiangxi Province, China, i.e. Peracarpa carnosa (Wall.) Hook. f. et Thoms., Listera japonica Bl., Odontochilus poilanei (Gagnepain) Ormerod, Odontochilus guangdongensis S. C. Chen et al., Ribes davidii Franch., and Ilex syzygiophylla C. J. Tseng ex S. K. Chen et Y. X. Feng. In addition, the genera Peracarpa Hook. f. et Thoms., Odontochilus Blume and Listera R. Br. had not been previously recorded in Jiangxi. References | Related Articles | Metrics

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Euphorbia graminea, a Newly Naturalized Species of Euphorbia from Chinese Mainland WU Bao-huan, SHI Wen-ting, LIU Chao-yu, YANG Hai-jun 2018, 47(04): 377-380.  DOI: 10.3969/j.issn.1009-7791.2018.04.013 Abstract ( )   PDF (1194KB) ( )   Euphorbia graminea Jacq. is newly reported to naturalized in Guangdong Province, China. This species is considered originated in area from Mexico to Northern South America, and it was recorded naturalized as weed in Pacific Islands, Nigeria, Italy and China’s Taiwan. References | Related Articles | Metrics

Plant cultivation and tissue culture

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Truncated Harvesting Technology of Cinnamomum burmannii chvar. Borneol WU Xian-ji, CHENG Yi-qun, CHEN Jie-lian, LAN Yan-qun, LIAN Hui-ming, HE Bo-xiang, ZHANG Qian, WANG Ying-li, CAI Yan-ling, LIN Sheng, YAO Yan-fei 2018, 47(04): 381-385.  DOI: 10.3969/j.issn.1009-7791.2018.04.014 Abstract ( )   PDF (823KB) ( )   In order to standardization of harvesting techniques for dwarf forest of Cinnamomum burmannii chvar. Borneol, the cutting and harvesting technology was studied in the 2-year-old young forest, 4 truncation heights were set, and 3 sub-branch processing modes were adopted to carry out each truncation height. Based on the analysis of three surveys in two years, results showed that there was no significant difference in background among four traits of tree height, ground diameter, crown width and steaming biomass. After treatment, the differences of four traits among different treatments were increased significantly, and all of them achieved very significant level. Multiple comparisons were also increased from 2 DUNNCAN groupings in the background to 3—4 groupings, and with the increase of truncation height, four trait indicators were gradually increased; the fewer retained branches, the four trait indicators were smaller. So the optimum processing combination was proposed that all branches of the trunk were retained after truncation at the height of 120 cm. References | Related Articles | Metrics

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Seed Germination and Seedling Growth Rhythm of Adansonia digitata QI Guo-hai, ZHANG Yan-ping, WU Jiang-chong, SUN Heng, ZHENG Yi-xing, LIU Zi-yi, YANG Ming, LIU Jian-jin, WANG Xue-jian, ZHANG Ti-kun 2018, 47(04): 386-390.  DOI: 10.3969/j.issn.1009-7791.2018.04.015 Abstract ( )   PDF (493KB) ( )   Seedling-raising experiments of Adansonia digitata seeds with three different pretreated conditions were carried out. Meanwhile, the seed germination characteristics, seedling heights, ground diameter and phenological characteristics of seedlings were observed during two years. The results showed that, (1) the highest seed germination rate and germination power were 18.72% and 6.20% by dipping in 70 ℃ water. (2)The seedling heights and ground diameter increments followed an ‘S’ type growth curve, including slow-growing stage, fast-growing stage, lag-growing stage. The seedling heights and ground diameter grow fast from early July to early September, and the fast-growing point was occurred in mid-August. While the annual growth of A. digitata seedling heights and ground diameter was 81.9 cm and 12.6 mm. The seedlings had a good uniformity and growth quality, and the survival rate of transplant could be above 90%. (3) Seedling phenological characteristics were no significant difference according to the phenological records in Yuanyang dry-hot valley areas during two years, and could growth normally, which indicated A. digitata would be introduced successfully in Yuanyang dry-hot valley areas and maybe introduced successfully in other similar areas. References | Related Articles | Metrics

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Tissue Culture and Rapid Propagation of Ornithogalum concodianum CHEN Han-xin, JU Yu-dong, WAN Xue-feng 2018, 47(04): 391-394.  DOI: 10.3969/j.issn.1009-7791.2018.04.016 Abstract ( )   PDF (376KB) ( )   The bulbs in the dormant period of Ornithogalum concodianum were used in vitro regeneration system study. The results showed that the medium MS (KH2PO4 doubled)+4.0 mg·L-1 6-BA+0.5 mg·L-1 NAA was suitable for induction of bulbs with 54.2% induction rate; 6-BA was the main factor affecting bulb proliferation, and the medium 2/3MS (KH2PO4 doubled)+4.0 mg·L-1 6-BA+1.0 mg·L-1 NAA+2% sucrose was suitable for bulb proliferation with proliferation coefficient 4.0; the medium 1/2MS (half of large element only)+1.0 mg·L-1 IBA+1.0 mg·L-1 NAA+1.0 g·L-1 AC promoted rooting. The substrate (grass charcoal : garden soil : volcanic rock= 5 : 1 : 1 in volume) was suitable for cultivation, the survival rate was reached 89.6%. References | Related Articles | Metrics

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Progress in Research of Palmitoylated Proteins and Protein Palmitoylation DING Yu-jiao, HAN Ying-ying, ZHOU Jing-wen 2018, 47(04): 395-403.  DOI: 10.3969/j.issn.1009-7791.2018.04.017 Abstract ( )   PDF (419KB) ( )   Protein S-palmitoylation, the most common lipid modification with the 16-carbon fatty acid palmitate, provided an important mechanism for regulating protein trafficking and function. This article summarized a family of conserved DHHC proteins with palmitoyltransferase activity that had been discovered from plants to mammals. The method of protein palmitoylation was so concluded. The paper also introduced the research methods of protein palmitoylation, the prediction methods of palmitoylation sites (CSS-Palm, NBA-Palm, TermiNator2), radioactive labeling (radiolabeling with 3H palmitate or 125I-IC16 palmitate) and non-radioactive labeling (chemical labeling and mass spectrometry) to detect palmitoylated proteins. Next, techniques to inhibit protein palmitoylation were described. These included site specific mutagenesis, and treatment of cells with inhibitors of protein palmitoylation, including 2-bromopalmitate, cerulenin, and tunicamycin. Based on the authors’ research directions, the response of protein palmitoylation in plant stress and the application prospect of plant stress resistance were summarized and prospected. References | Related Articles | Metrics