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Effect of Aluminum Toxicity Stress on Growth and Physiology of Michelia chapensis Seedlings
GONG Hai-guang,JIANG Chun-ning,HUANG Yong-fang,LI Yan
2015, 44(02):
96-100.
DOI: 10.3969/j.issn.1009-7791.2015.02.002
To study the effect of aluminum stress on the growth of Michelia chapensis seedlings, the seedlings were cultivated with water culture with different Al3+ concentration of 0.5 (T1), 1 (T2), 2 (T3) and 0 mmol·L-1 (CK), and the root length, content of aluminum in the roots’ tip, the activity of POD and CAT, the content of MDA and proline were measured. The results showed that the elongation of M. chapensis root was inhibited significantly cultured with Al3+ for 24 hours, with the increasing of the concentration of aluminum, the degree of inhibition was rising. The relative elongation of roots was 84.53%, 63.16% and 54.46%, respectively. The content of aluminum in the root tip of M. chapensis was increased with the increasing of treatment time and aluminum density; after 6 days, the concentration of Al were 63.64, 82.6, 121.69 times of control, respectively. Stressed by the aluminum, the activity of POD and CAT increased with the increasing of time and aluminum density. The activity of POD and CAT in 6th day respectively increased 17.00%—115.21% and 25.11%—49.28% compared with 1st day. The MDA and proline were increased with the increasing of time and aluminum density. In 6th day, MDA in roots cultured in T1, T2, T3 increased 46.13%—59.78%, and proline increased 60.12%—150.85% compared with control. With aluminum stress, the content of aluminum in root tips of M. chapensis increased and elongation of its roots was inhibited; M. chapensis activated antioxydase system to defense the aluminum stress, and generated proline to regulate osmotic pressure.
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