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28 February 2024, Volume 53 Issue 1 Previous Issue    Next Issue
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Identification and Expression Analysis of PYL Gene Family in Eriobotrya japonica LIN Qiu-xiang, HAN Yu-xin 2024, 53(1): 1-11.  DOI: 10.3969/j.issn.1009-7791.2024.01.001 Abstract ( )   PDF (452KB) ( )   Abscisic acid (ABA) is one of the important hormones regulating plant growth and development, and abiotic stress. As an ABA receptor, PYL is located at the top of its signaling pathway and plays a core regulatory role. However, this gene family has not been identified and analyzed in detail in loquat. In this study, 12 PYL genes were identified in loquat based on bioinformatics analysis. The molecular weight of the coding PYL protein ranged from 10.47 to 29.12 kDa, and most of them were localized in the cytoplasm. PYL family genes were distributed on 8 chromosomes. Phylogenetic tree analysis showed that PYL gene family could be divided into three subfamilies and EjPYL1 was closely related to AtPYL13. PYL proteins in loquat were consisted of motif 1-6, and motif3 was the common domain. Genes of PYL family were collinearity in species loquat. There were 2 pairs of collinearity between loquat and Arabidopsis thaliana. Cis-components in promoter of PYL genes were related to low temperature, drought, light and hormone response. RNA-seq of loquat young fruits indicated that EjPYL6 and EjPYL8 were both up-regulated by low temperature in fruit pulp and seeds. This study can provide theoretical basis for further understanding ABA signal transduction pathway and breeding new varieties of loquat with frost resistance. References | Related Articles | Metrics

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Physiological Response of Ludisia discolor Seedlings to Drought Stress and Screening of Drought Resistance Indicators XU Li-li, MENG Xin-ya, YOU Yan-ping, SONG Xi-qiang, CHEN Yao-li, ZHONG Yun-fang 2024, 53(1): 12-21.  DOI: 10.3969/j.issn.1009-7791.2024.01.002 Abstract ( )   PDF (500KB) ( )   In order to investigate the tolerance of Ludisia discolor to drought stress, the experiments potted water control to simulate drought were used to study the changes of growth, physiological and biochemical indicators, and bioactive components in L. discolor seedlings with around 6 months of age under different water treatments. The main response factors to drought resistance were evaluated through correlation analysis (CA) and principal component analysis (PA), which would provide some references for the water management under conservation and cultivation of L. discolor. The results showed that with the increase of drought severity, the height of L. discolor seedlings showed a downward trend compared to the control group, but there was no significant effect on stem thickness. The relative water content of leaves decreased, and the content of chloroplast pigment (Chl) first increased and then decreased, while the content of anthocyanin (ACN) showed an upward trend. Under drought stress, the activities of peroxidase (POD) and superoxide dismutase (SOD) showed a trend of first increasing and then decreasing. The contents of malondialdehyde (MDA), soluble sugar (SS), and the bioactive substances such as total flavonoids (TF), total phenols (TP), and polysaccharides (PS) all increased. Through correlation analysis and principal component analysis, it was found that MDA, SS, PS, and relative water content could be used as the preferred evaluation indicators, and in addition, stem thickness and SOD activity could be used as auxiliary indicators for drought resistance evaluation in L. discolor. In summary, the results indicated that moderate drought stress (soil moisture content 50%–55%) could promote the synthesis and accumulation of osmotic regulating substances and bioactive components of L. discolor, At the same time, to improve the quality of L. discolor, it was recommended to carry out short-term severe drought (soil moisture content 15%–20%) before harvest so that the biological activities of L. discolor could be enhanced. These findings had practical guiding significance for cultivation management. References | Related Articles | Metrics

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Differences in Growth and Metabolics between Hydroponic and Peat Soil Cultivation Anoectochilus roxburghii ZHAO Qi, WANG Chi, ZHAO Feng 2024, 53(1): 22-30.  DOI: 10.3969/j.issn.1009-7791.2024.01.003 Abstract ( )   PDF (419KB) ( )   The aim of the study was to systematically compare the differences in growth indexes and quality components between hydroponics and soil cultivation for 30 days and 90 days, and to investigate the main differential metabolic pathways of Anoectochilus roxburghii. The results showed that comparing with soil cultivation, the root length of hydroponics increased significantly on 30th day, and the root length and leaf area increased significantly on 90th day, but the stem thickness decreased. The net photosynthetic rate increased significantly on 30th day of hydroponics, and the chlorophyll content increased significantly on 90th day of hydroponics. In terms of components, total flavonoids and kinsenoside increased significantly on 30th day of hydroponics, and aspartic acid, γ-aminobutyric acid, and hydroxyproline decreased significantly; aspartic acid, asparagine, γ-aminobutyric acid, and methionine decreased significantly on 90th day of hydroponics. On metabolic pathways, lipid, flavonoid and flavonol biosynthesis, diterpene and secondary metabolic biosynthesis were significantly up-regulated on 30th day, and lipid and secondary metabolic pathways were significantly up-regulated, while diterpene biosynthesis underwent a down-regulation on 90th day in the hydroponically grown group compared to the soil-grown group on 30th day and 90th day. References | Related Articles | Metrics

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Mechanism of Traditional Chinese Medicine Genkwa Flos for Treatment of Primary Dysmenorrhea Based on Network Pharmacology and Molecular Docking LI Si-meng, TIAN Rong, YIN Ming-jing, GU Wei 2024, 53(1): 31-39.  DOI: 10.3969/j.issn.1009-7791.2024.01.004 Abstract ( )   PDF (470KB) ( )   The effective material basis and potential mechanism of Genkwa Flos in treating primary dysmenorrhea by network pharmacology and molecular docking techniques were studied. Based on the TCMSP database, the active substances and targets of Genkwa Flos were predicted, and the targets related to primary dysmenorrhea were obtained with the help of DisGeNET and other databases. Based on the intersection targets of Genkwa Flos and primary dysmenorrhea, the intersection target protein interaction network was constructed using the STRING database, and the “Genkwa Flos-active substances-target-primary dysmenorrhea” network was constructed using Cytoscape3.7.2. Gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis were performed using the DAVID database, and molecular docking of the core targets and corresponding active ingredients was performed using Autodock tools. Results showed that the core active substances of Genkwa Flos for treating primary dysmenorrhea are Genkwanin, (2R)-5,7-dihydroxy-2- (4-hydroxyphenyl) chroman-4-one, 7-hydroxy-6-methoxy-3,7’-dicoumarin, Gentiacaulein, Hydroxygenkwanin, kaempferol, luteolin, sitosterol, 9-hydroxyglabratolide; key targets include ESR1, PTGS1, PTGS2, GSTM1, CYP1A1, OPRM1, NTRK2 and NR1H2; the associated biological processes are mainly related to aging, positive regulation of nitric oxide biosynthetic process, cytoplasm and ATPase binding; thepathway of KEGG are mainly enriched in the pathogenesis of cancer, chemical carcinogenesis-receptor activation, and chemical carcinogenesis- DNA adducts. In addition, molecular docking shows that the docking energy values between the core active ingredient Genkwanin and the key target proteins are all less than –7.0 kcal·mol–1, which have strong binding activity and can form stable structural conformations. This study used network pharmacology and molecular docking methods to preliminarily explore the active material basis of Genkwa Flos for treating primary dysmenorrhea from the perspective of “Chinese medicine-ingredient-target-disease”, laying a foundation for further studying the mechanism of action of this kind of ingredients for treating dysmenorrhea. References | Related Articles | Metrics

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Effects of Soil Amendments on Soil Environment and Baby Bok Choy Quality WEI Jin-yi, WANG Ming-yuan, TANG Yi, WANG Zi-shu, YANG Chen-yi 2024, 53(1): 40-45.  DOI: 10.3969/j.issn.1009-7791.2024.01.005 Abstract ( )   PDF (642KB) ( )   In order to explore the effects of soil amendments on the soil environment, quality and yield of the Baby Bok Choy, the soil amendments developed by our laboratory were used as materials, and soil pH, organic matter content and the internal and external quality of ‘Xiaobaimiao’ cabbage were studied. Four treatments were designed as follows: no soil amendments (CK), and application of the soil amendments 25 kg (B25), 50 kg (B50) and 75 kg (B75) per 667 m2 of vegetable garden. The results showed that the application of soil amendments could maintain soil pH 7.0 above and improve soil acidification. The organic matter content in different soil layers decreased with the deepening of the soil layer. In the soil layer of 31–45 cm, the highest organic matter content was observed in the B25 treatment, which was 5.35 g·kg–1, significantly higher than that in the CK group (P<0.05); soil amendments could increase the content of Vᴄ, soluble protein, and free amino acids in the Baby Bok Choy, while reducing nitrate content. Among them, under B75 treatment, the content of Vᴄ, soluble protein, and free amino acids was the highest, significantly different from the CK group (P<0.05), and the nitrate content was the lowest, 0.4713 mg·g–1, significantly different from other treatments (P<0.05). Moderate application of soil amendments could increase the length, width, and number of leaves of the Baby Bok Choy, and improve its external quality. Under B25 and B50 treatments, the fresh weight of the Baby Bok Choy above ground increased by 79 g and 16 g compared to the CK group, respectively, with significant differences (P<0.05). The application of soil amendments can improve the yield and quality of the Baby Bok Choy, and improve the soil environment. References | Related Articles | Metrics

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Characteristics of Population Structure and Growth Status of Syzygium tephrodes in Jianfengling Tropical Rainforest, Hainan, China XIA Lian, XU Han, LI Yan-peng, QIN Wen-hao 2024, 53(1): 46-52.  DOI: 10.3969/j.issn.1009-7791.2024.01.006 Abstract ( )   PDF (615KB) ( )   In this study, we took Syzygium tephrodes as the object in a 60 hm2 large sample plot in Jianfengling, Hainan, and investigated the structural characteristics and growth dynamics of its population, as well as the intensity of inter-plant competition and its distribution pattern. The results showed that: (1) S. tephrodes populations have strong natural renewal ability, abundant seedlings, and the mortality rate of the populations increased or decreased with the increase of the diameter level and then decreased rapidly at the Ⅳ stage, and the fluctuation amplitude was the most obvious at the Ⅳ–Ⅴ diameter level, and the mortality rate declined rapidly after the Ⅳ level and tended to be stabilized. (2) The survival curves of S. tephrodes were of Deevey-Ⅲ type, the mortality rate was high in the early stage, and low once it reached a certain age. (3) The results of the time series prediction showed that the number of individuals in each age class increased to different degrees after the next 2, 4 and 6 age classes. In conclusion, the mortality rate of young individuals in S. tephrodes population was high, but there was still a certain potential for renewal. This study provided theoretical support for the landscaping application and protection strategy formulation of S. tephrodes. It was recommended to further strengthen habitat protection and take appropriate intervention measures to promote stable population development within the community. References | Related Articles | Metrics

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Preliminary Study on Bryophyte Diversity in Medog of Xizang, China MA He-ping, SI Meng-xin, GAO Zhi-yuan 2024, 53(1): 53-59.  DOI: 10.3969/j.issn.1009-7791.2024.01.007 Abstract ( )   PDF (492KB) ( )   Medog is rich in plant species due to its special geographical location and huge elevation difference. Its unique physical geography and climatic conditions give birth to a variety of habitats for the distribution of bryophytes and form a diverse bryophyte community. In order to study the bryophyte diversity and its life-form composition in Medog of Xizang, line collection methods was used to investigate and collect bryophyte specimens, species identification and correlation analysis. The results showed that: (1) the present checklist includes 631 species belonging to 239 genera in 83 families, with 2 hornwort species in 2 genera and 2 families, 217 liverwort species in 68 genera and 38 families, and 412 moss species in 169 genera and 43 families. There are 19 dominant families, 26 dominant genus and 19 dominant species of bryophytes in this area. (2) There are 327 species belonging to Soil and Rock-community, accounting for 51.83% of the total bryophytes species in Medog; There are 216 species belonging to Rock-community, accounting for 34.23% of the total bryophytes species in the area; there are 173 species in Soil-community, accounting for 27.42% of the total bryophytes species. There are 97 species in Tree-community, accounting for 15.37% of the total bryophytes species in this area, and 21 species in Leaf epiphyte-community, accounting for 3.32% of the total bryophytes species in this area. Overall, because of its good natural environment conditions, Medog provides good conditions for the survival of rich bryophytes. References | Related Articles | Metrics

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New Data of Salvia from Zhejiang Province LI Jun-ping, ZHANG Pei-lin, ZHONG Jian-ping, ZHU Yi-rong, XU Liang-dong, ZHU Hao-dong, XIE Wen-yuan 2024, 53(1): 60-63.  DOI: 10.3969/j.issn.1009-7791.2024.01.008 Abstract ( )   PDF (276KB) ( )   The classification below Salvia sinica Migo (Lamiaceae) was studied in this paper. The form rake of S. sinica f. purpurea H. W. Li was restored, and S. sinica f. flaviflora J. P. Li, Y. R. Zhu et Z. H. Chen, as a new form from Zhejiang Province was reported. The voucher specimens were deposited in Zhejiang Museum of Natural History (ZM). References | Related Articles | Metrics

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Four Newly Recorded Species of Angiosperms from Anhui Province FANG Cheng-cheng, CHEN Ya-nan, LI Si-rong, ZHA Rui, ZHANG Wei 2024, 53(1): 64-67.  DOI: 10.3969/j.issn.1009-7791.2024.01.009 Abstract ( )   PDF (258KB) ( )   Through field survey and specimen identification, four species of angiosperms are confirmed as new distribution records from Anhui Province, belonging to four genera in four families, namely Persicaria debilis (Meisn.) H. Gross ex W. Lee, Lactuca serriola L., Carex baohuashanica Tang et Wang ex L. K. Dai, and Vitis shizishanensis Z. Y. Ma, J. Wen, Q. Fu & X. Q. Liu. The voucher specimens are stored in the Herbarium of the College of Life Sciences, Anqing Normal University. References | Related Articles | Metrics

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Calyptocarpus, a Newly Naturalized Genus of Asteraceae in Guangdong HE Chun-mei, TONG Yi-hua 2024, 53(1): 68-71.  DOI: 10.3969/j.issn.1009-7791.2024.01.010 Abstract ( )   PDF (345KB) ( )   This paper reported a newly naturalized genus of Asteraceae, Calyptocarpus Less. in Guangdong. Calyptocarpus vialis Less. is native to the southern United States, Mexico and Cuba, and was previously reported to be naturalized in China (Taiwan, Yunnan and Guangxi), Indonesia (Java), Oceania, India and Africa. Many populations of this invasive species were discovered in Chebei Chong, Guangzhou. It grows vigorously and tends to take over the habitat of other native herbaceous plants, which seems to be an invasive species and should attract the attention of relevant departments. Calyptocarpus vialis is similar to Synedrella nodiflora (L.) Gaertn., but markedly differs by having prostrate stems, homogeneous achenes with wingless ray ones, and palea with an acute apex. The vouchers are deposited in the herbarium of South China Botanical Garden, Chinese Academy of Sciences (IBSC). References | Related Articles | Metrics

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Application of Horticultural Therapy to Improvement of College Students' Psychological Quality GUO Can-li, XIE Han, WANG Li-xian, LI Dong-ze 2024, 53(1): 72-79.  DOI: 10.3969/j.issn.1009-7791.2024.01.011 Abstract ( )   PDF (531KB) ( )   This paper explores the benefits of alleviating college students' mood and improving their psychological quality, expected to provide reference for the popularization and application of horticultural therapy in colleges and universities. Through 10 types of horticultural activities, and the POMS scale and satisfaction survey were used for quantitative and qualitative analysis. The results indicate that the mood state TMD of participants in 10 horticultural activities decrease, with an average decrease of 12.68; Activities such as plant recognition, leaf vein bookmarking, plant sowing, plant rubbing, succulent planting, and sachet making can significantly improve the negative emotions of college students; Various horticultural activities have a good effect on improving specific mood states such as fatigue (2.50), depression (2.05), and tension (1.91). Among them, plant rubbing activities have the best effect and can significantly improve 6 mood states; The average overall satisfaction evaluation of various horticultural activities is 8.10, indicating a good overall evaluation, with higher satisfaction rates for sachet making and succulent planting. This study indicates that each horticultural activity has a certain improvement effect on the participants' emotions. Plant recognition, plant sowing, succulent cultivation, and sachet making improve the participants' psychological status well, conclusion that horticultural therapy can improve the psychological quality of college students to a certain extent. It is feasible to apply horticultural therapy to college students' mental health education. References | Related Articles | Metrics

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Research Advances in Metabolic Regulation, Storage and Transport of Carotenoids in Plants YU Yin-feng, LIU Qing-qing, LIU Xiao-chun, ZHANG Da-sheng, CUI Li-jie 2024, 53(1): 80-88.  DOI: 10.3969/j.issn.1009-7791.2024.01.012 Abstract ( )   PDF (367KB) ( )   Carotenoids are a group of natural pigments widely found in plants, which are generally composed of C40 terpenoids and their derivatives, which are connected from end to end of 8 isoprene units. In plants, carotenoids not only make plant organs appear color, but also perform important biological functions. In recent years, although the biosynthesis pathway of carotenoids is relatively clear, the mechanisms of metabolic regulation, including storage and transport of carotenoids in vivo are not yet understood. This paper briefly summarized the research progress of the biosynthesis, storage localization of carotenoids and terpenoid transporters, and focused on the potential substances that mediate carotenoid transport and the methods of identifying carotenoid transporters, in order to provide a better understanding of carotenoid synthesis and transportation. References | Related Articles | Metrics