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10 March 2011, Volume 40 Issue 01 Previous Issue    Next Issue

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Cloning Vernalization-Related Gene VRN2 and Construction of its Plant Expression Vector in Arabidopsis thaliana ZHANG Wen-hui, HONG Li-ping, ZENG Bi-yu 2011, 40(01): 1-4.  DOI: 10.3969/j.issn.1009-7791.2011.01.001 Abstract ( )   PDF (1346KB) ( )   In this paper, the vernalization-related Gene VRN2 cDNA from RNA of Arabidopsis thaliana L., GeneBank accession number AY063047 was successfully cloned.The cDNA sequence of VRN2 was 1 354 bp and its region in encoding was 1323 bp.The protein deduced from VRN2 contained 441 amino acids.The cloned cDNA of VRN2 was introduced into a middle vector pBPFΩ7.The fragment with P35S promoter and nos were digested by PstI, which were connected to pBI121 vector, giving plant espression vector containing VRN2 gene. Related Articles | Metrics

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Effects of Different Substrates and Growth Regulators on Photosynthesis and Water Use Efficiency of Artocarpus lingnanensis CHEN Xiang, LU Yao-dong, KE Huan, TIAN Xue-qin, ZHANG Xue-ping, TAN Jia-de 2011, 40(01): 5-8.  DOI: 10.3969/j.issn.1009-7791.2011.01.002 Abstract ( )   PDF (860KB) ( )   The effects of different substrates and growth regulators on photosynthesis and water use efficiency (WUE) of Artocarpus lingnanensis were studied in this paper.The results showed that net photosynthetic rate (Pn), transpiration rate (Tr), WUE of A.lingnanensis cultivated in improvement substrate (S2) with appropriate IBA and NAA were enhanced.The assimilation products of A. lingnanensis were remarkable enhanced in S2 compared with in S1, and Pn, stomatal conductance (Gs), Tr, WUE and chlorophyll b (CHLb) were also significant raised;Pn, WUE, total chlorophyll (TCHL), chlorophyll a (CHLa), ChLb of treatment with appropriate IBA and NAA were significantly higher than that of CK and IBA treatment.The results indicated that S2 with appropriate IBA and NAA was the best treatment of to improve photosynthesis and assimilation of A. lingnanensis. Related Articles | Metrics

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The Effect of Cd-Pb Compound Stress on the Protective Enzyme of Polystichum neolobatum and Dryopteris fuscipe LIAO Xue-lan, PAN Yuan-zhi, YU Yan, ZHOU Feng-rong 2011, 40(01): 9-12.  DOI: 10.3969/j.issn.1009-7791.2011.01.003 Abstract ( )   PDF (789KB) ( )   Fern is an old fibrovascular plant and also a unique species in the evolution of botany system.The active change of the protective enzyme of Polystichum neolobatum and Dryopteris fuscipes under the compound stress of different Cd-Pb concentrations was studied in this article.It was discovered that the activeness of Polystichum neolobatum's protective enzymes, POD, SOD and CAT, had a positive collaboration with the given concentration.In other words, with the increasing of given concentration, the activeness of enzyme also increased evidently.The activeness of Dryopteris fuscipes' protective enzymes, POD, SOD, CAT, was repressed in the given concentration of 600+1200mg/kg, while was promoted in 200+400mg/kg and 400+800 mg/kg. Related Articles | Metrics

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Sap Flow of Pinus massoniana Responses to the Typhoon in Serious Soil Erosion Areas,Changting County of Fujian Province ZHENG Huai-zhou, ZHU Jin-mao, LI Shou-zhong, WEI Xia, YUAN Hong-wei, Fang Zhou-yi 2011, 40(01): 13-17.  DOI: 10.3969/j.issn.1009-7791.2011.01.004 Abstract ( )   PDF (1075KB) ( )   Application of thermal diffusion technology to measure the sap flow of Pinus massoniana in serious soil erosion areas in Changting county during the typhoon, the results showed that:typhoon brought high-intensity rainfall and strong winds which suppressed transpiration;the rainfall didn't improve plant availability moisture;photosynthesis and water physiology before and after the typhoon had no significant difference;the sap flow density during the typhoon was significantly related (P<0.01) with the environmental factors as:solar radiation, air temperature and vapor pressure deficit, while on sunny days were:air temperature, solar radiation, relative humidity and vapor pressure deficit.Studies have shown that:typhoon did not improve the plant available water, nor destroy the physiological activities of Pinus massoniana. Related Articles | Metrics

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Study on Graduation and Comprehensive Evaluation of Tree Species Wind-resistance in Zhanjiang,Guangdong Province WU Zhi-hua, LI Tian-hui, ZHANG Hua-lin, XIE Yao-jian 2011, 40(01): 18-23.  DOI: 10.3969/j.issn.1009-7791.2011.01.005 Abstract ( )   PDF (998KB) ( )   In terms of tree type, tree height, diameter at breast height, crown width, stem strength, wood density, foliage layer and root morphology, which were all related with the wind-resistance, graduation and comprehensive evaluation of tree species wind-resistance were studied by comprehensive evaluation and grey relational grade method in this paper.For 82 tree species in Zhanjiang city, the classification result was that:They were classified into four classes including class Ⅰ with 19 species, class Ⅱ with 20 species, class Ⅲ with 31 species and class Ⅳ with 12 species, and grey relational grade was much fitter to evaluate and grade the wind-resistance of tree species than comprehensive evaluation. Related Articles | Metrics

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Study on Carbon Sequestration of Vegetation in Eco-Economic Zone of Poyang Lake JIN Shu-lan, ZHU Zi-ming, XU Cai-qiu 2011, 40(01): 24-27.  DOI: 10.3969/j.issn.1009-7791.2011.01.006 Abstract ( )   PDF (863KB) ( )   According to the study on carbon sequestration of vegetation in Eco-economic Zone of Poyang Lake, the total carbon storage of forest is 364.3×106(t), the carbon sequestration of grassland vegetation is 90.65×104 (t), the carbon sequestration of rice is 18.51×106 (t), the carbon sequestration of other crops is 20.64×106 (t).The total CO2 storage of wetland is 609120t/a in Poyang Lake.There were some suggestions given, includes:For existing forest, tending wood scientifically and felling reasonably to form rational tree species structure, age structure and Lin layer structure, to improve the stability of forest ecosystem and carbon absorbing ability.Use the conservation tillage, water-saving irrigation and fertilization techniques, and to cultivate the new nitrogen efficient utilization of agricultural varieties, to improve photosynthesis and enhance carbon sequestration ability of local crops.Graze and ecological monitor the grassland resources rationally, control the size and number of breeding strictly, so as to achieve the goal of the grassland ecosystem in reducing and CO2 fixation.Prohibit silted up, perfect environment monitoring network, and also protect water wetland ecological functions. Related Articles | Metrics

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Research on the Antimicrobial Activities of Extracts from Gelidium amansii and Sargassum pallidum LIN Xiong-ping, ZHOU Feng-fang, CHEN Xiao-qing, PENG Biao 2011, 40(01): 28-30.  DOI: 10.3969/j.issn.1009-7791.2011.01.007 Abstract ( )   PDF (764KB) ( )   Crude extracts and fractions separated from ethanol extracts from Gelidium amansii and Sargassum pallidum were tested for their efficacy against bacterial by the circle agar disc diffusion method.As a result, Gelidium amansii and Sargassum pallidum had different degree of antimicrobial activitics.The antibacterial activities of crude extracts from Sargassum pallidum were stronger than antifungal activities.The ethanol extracts from Gelidium amansii showed the antifungal activities against Aspergillus niger with the inhibition zone 4 diameters, which were stronger than the antibacterial activities against Bacillus subtilis, Escherichia coli and Staphylococcus aurens. Related Articles | Metrics

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Investigation and Analysis of Heavy Metal Pollution of Banana Soil in Zhangzhou,Fujian Province LIN Xiang-hua, LIU Jia-min 2011, 40(01): 31-33.  DOI: 10.3969/j.issn.1009-7791.2011.01.008 Abstract ( )   PDF (749KB) ( )   By investigating the contents of heavy metals as Cd, Pb, Hg, Cr, Cuand the harmful elements As heavy metal content in banana soil of Zhangzhou, Fujian province, evaluating them by taking the ways of single pollution index and comprehensive pollution index.It shows that, by taking the background value of soil of Fujian as the evaluation standard, soil of some of the banana lands of Zhangzhou are polluted by heavy metals with a pollution order of single pollution index as Hg (1.60) >Pb (1.26) >Cd (1.07) >Cu (0.76) >As (0.50) >Cr (0.38) and the main pollution elements as Cd, Pb and Hg, of which the pollution of Cd and Hg are moderate pollution;while the comprehensive pollution index is 1.16 that they rank light grade.By taking the second grade standard of soil environmental quality as the evaluation standard, the comprehensive pollution index is 0.34 that they rank non-pollution grade.Different sampling points on heavy metal pollution in a comparative analysis shows the road traffic and garbage and sewage on the banana plantations in the soil are caused by different levels of heavy metal pollution, mainly as Cd, Pb, Hg pollution. Related Articles | Metrics

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Tissue Culture of Brassica alboglabra CHEN Qiong-e, ZHONG Feng-lin, LIN Bi-ying 2011, 40(01): 34-37.  DOI: 10.3969/j.issn.1009-7791.2011.01.009 Abstract ( )   PDF (955KB) ( )   This experiment focused on testing the most suitable substrate for the tissue culture of Brassica alboglabra.The caulis segments and hypocotyl were separated from aseptic seedlings and used as explants.The results showed the best culture medium for hypocotyl was MS+6-BA 3.0 mg/L+NAA 0.2 mg/L, and for the caulis segments was MS+6-BA 5.0 mg/L+NAA 0.2 mg/L.The most suitable substrate for multiplication of adventitious buds was MS+6-BA 3.0 mg/L+NAA 0.2 mg/L, and the substrate with 1/2 MS+NAA 0.1 mg/L+IBA 0.2 mg/L had the best performance for rooting culture.The survival percentage of transplanting seedlings reached 96.6%. Related Articles | Metrics

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The Effect of KT and NAA on Redifferentiation of Anemarrhena asphodeloides Callus HONG Sen-rong, XIONG Xi 2011, 40(01): 38-40.  DOI: 10.3969/j.issn.1009-7791.2011.01.010 Abstract ( )   PDF (865KB) ( )   The effects of concentrations of KT and NAA on redifferentiation of Anemarrhena asphodeloides callus were studied using plant tissue culture methods and single-factor test.The results showed:The optimal rooting medium of A. asphodeloides callus was MS+KT 2 mg/L.The optimal regenerated shoot medium of A. asphodeloides callus was MS+KT 2 mg/L+NAA 1 mg/L.The optimal seedling growth medium of A. asphodeloides callus was MS+KT 2 mg/L+NAA 0.5 mg/L.The optimal proliferation medium of A. asphodeloides callus was MS+KT 2 mg/L+NAA 1 mg/L. Related Articles | Metrics

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The Effects of KT and NAA on Proliferation of Astragalus membranceus Stem with a Bud and its Induction of Callus and Rooting YIN Ming-hua, CAO Zhen-long 2011, 40(01): 41-44.  DOI: 10.3969/j.issn.1009-7791.2011.01.011 Abstract ( )   PDF (890KB) ( )   Through the establishment of tissue culture rapid propagation system, the effects of KT and NAA on the induction of new stem, callus and roots of Astragalus membranceus were studied.The induction of new stem was related to the ratio of KT/NAA, which was obvious with the average number of new stem was the maximum, 11.7, when the value was 4.With the promotion of NAA, lots of white callus was produced.The average diameter of white callus, which was in the medium with 1 mg/L of NAA, was 1.4 cm.Roots came mainly from the white callus, being promoted by NAA but inhibited by KT.1 mg/L of NAA played a key positive role in the increase of roots number, the average length of roots and the number of secondary roots, but negative in roots average diameter. Related Articles | Metrics

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Investigation of Vascular Plant Resources in Foshan City, Guangdong Province LIAO Hao-bin, LAI Huan-wu, ZHAO Sheng, YANG Zhi-yan, FENG Zhi-jian, ZHOU Bin 2011, 40(01): 45-48.  DOI: 10.3969/j.issn.1009-7791.2011.01.012 Abstract ( )   PDF (848KB) ( )   The current status of the vascular plants in Foshan City, Guangdong Province was reported in this paper.According to the inverstigation, a total of 1813 species belonging to 832 genera, 239 families of vascular plants are found in Foshan City.Among them, there are 96 species of Pteridophytas belonging to 63 genera in 40 families, 41 species of Gymnospermaes belonging to 28 genera in 13 families and 1676 species of Angiospermaes belonging to 979 genera in 278 families.There are also lots of wild plants in Foshan City, including 421 medicinal plants, 100 edible plants, 24 aromatic plants, 61 oil-bearing plants, 61 fiber plants, 8 starchiness plants, 1 resin plant, 30 tannic plants, 8 pigment plants, 90 timber plants and 123 ornamental plants.Among those, there are 15 national key protected plants and 20 rare or endangered plants classified as red list including Cibotium barometz, Alsophila spinulosa and so on.The suggestions of plant resources exploitation and protection were put out according to the natural and social conditions in Foshan City. Related Articles | Metrics

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The Plant Resources of Liliaceae and Orchidaceae from Mt.Maluanshan in Shenzhen,China and their Protections XU Xiao-hui, FAN Qiang, LAI Yan-ling, SUN Yan-jun, YE Chang-jing, LIAO Wen-bo 2011, 40(01): 49-55.  DOI: 10.3969/j.issn.1009-7791.2011.01.013 Abstract ( )   PDF (993KB) ( )   Liliaceous and Orchidaceous plants are important plant resources and have positive significance for studying the system evolution and species diversity.In this paper, plant resources of Liliaceae and Orchidaceae from Mt.Maluanshan in Shenzhen, China have been investigated and studied, the resutes showed that Liliaceous plants has 14 species of 9 genera, which account for 64.3% and 70.0% of Liliaceous total number in Shenzhen;and Orchidaceous plants has 28 species of 22 genera, which account for 68.8% and 54.9% of Orchidaceous total number in Shenzhen.Based on the study of Liliaceae, Orchidaceae plants of Fenghuangshan Forest Park (3 genera and 3 species of Liliaceae;4 genara and 4 species of Orchidaceae), Tiegang Forest Park (3 genera and 3 species of Liliaceae;4 genara and 4 species of Orchidaceae), Tanglangshan Country Park (5 genera and 5 species of Liliaceae;8 genara and 9 species of Orchidaceae), Wutongshan Scenic Area (8 genera and 12 species of Liliaceae;17 genera and 30 species of Orchidaceae), Paiyashan Nature Reserve (7 genera and 8 species of Liliaceae;26 genara and 35 species of Orchidaceae), Qiniangshan Country Park (9 genera and 10 species of Liliaceae;21 genara and 30 species of Orchidaceae), whitch are on the central axis of the Shenzhen region from west to east, combined with the ecological environment feature, indicating the indicative significance in ecological environment and forest vegetation.Hereby authors put forward some ways for strengthening protection, management and research. Related Articles | Metrics

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Study on Plant Diversity of Changzhou Island,Guangzhou ZHOU Jing-song, WANG Shu-tian, LIU Li-cheng 2011, 40(01): 56-60.  DOI: 10.3969/j.issn.1009-7791.2011.01.014 Abstract ( )   PDF (812KB) ( )   Based on the field survey, the plant diversity of Changzhou Island, Guangzhou was studied.There are a total of 487 vascular plant species belonging to 358 genera, 127 families on the island, and the dominating vegetation are secondary evergreen broad-leaved forest and artificial vegetation.The diversity and geographical area of the wild plant on Changzhou Island were analyzed, the results indicated that few families are the dominant components of flora, and tropical elements predominate in the flora but there is a degree influence of temperate elements.Finally, suggestions for the protection of plant diversity were proposed. Related Articles | Metrics

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Investigation on Woody Medicinal Plant Resources in Yongchun County of Fujian Province WU Li-qing 2011, 40(01): 61-64.  DOI: 10.3969/j.issn.1009-7791.2011.01.015 Abstract ( )   PDF (872KB) ( )   Yongchun County, locating in the transition between mid-subtropical and south subtropical areas, is rich in woody medicinal plant resources.Based on the field investigation, specimen collection and identification, and a large amount of references, the status of woody medicinal plants was studied.There are a total of 129 species (including varieties) woody medicinal plants in 90 genera of 45 families.The medicinal portions of woody medicinal plants and their corresponding functions were introduced briefly, and the suggestions about their protection and utilization were also put forward. Related Articles | Metrics

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New Distribution Record of Angiosperm in Fujian CHEN Yong, WU Fa-wang 2011, 40(01): 65-66.  DOI: 10.3969/j.issn.1009-7791.2011.01.016 Abstract ( )   PDF (709KB) ( )   According to the investigation on the vegetation in Mingdong District of Fujian Province, a newly recorded genus, Phalaris Linn., and four newly recorded species of Angiosperm including Phalaris arundinacea Linn., Saxifraga zhejiangensis Z. Wei. et Y. B. Chang, Brasenia schreberi J. P. Gmel., and Euphorbia maculate Linn. were found in Fujian.The voucher specimens are deposited in the Herbarium of Ningde Normal University (NDTC). Related Articles | Metrics

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Application Research on Standardization Technology of Banana Production WEI Shao-long, DENG Li-guo, LU Shao-feng, LIN Gui-mei, ZOU Yu, LI Xiao-quan, ZHANG Jin-zhong 2011, 40(01): 67-69.  DOI: 10.3969/j.issn.1009-7791.2011.01.017 Abstract ( )   PDF (744KB) ( )   The general situation of banana production, and the banana industry key link's standardization technology and application were introduced in this article, such as plantlet production technology, precision planting technology, and fruit protection technology.The planting trial showed the competitiveness in banana market could be advanced by standardization technology applied for banana planting. Related Articles | Metrics

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Quality Evaluation of Fructus viticis Commodity in Fujian Province LIU Xiao-fen, YANG Cheng-zi, FAN Shi-ming, YE Meng-ping 2011, 40(01): 70-74.  DOI: 10.3969/j.issn.1009-7791.2011.01.018 Abstract ( )   PDF (821KB) ( )   To provide the reference for making quality standard of Fructus viticis drug, the quality of 16 Fructus viticis commodities from different drugstores in Fujian province were analyzed and evaluated.The source and trait of Fructus viticis commodities form different cities were collected and studied, and the contents of total ash, methanol-extract, total flavones, vitexicarpin were determined.The results showed that the quality of Fructus viticis commodities were influenced by manufacturing location, batch and the process of preparing Chinese medicine.It is necessary to make strict quality evaluation and control system at intermediate links to guarantee the effectual use of drug ingredients. Related Articles | Metrics

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Industry Status and Development Trend of Blueberry in Hubei Province YANG Fu-chen, TU Jun-fan, QIN Zhong-qi, LIU Xian-qin, LI Xian-ming, ZHU Hong-yan 2011, 40(01): 75-78.  DOI: 10.3969/j.issn.1009-7791.2011.01.019 Abstract ( )   PDF (831KB) ( )   The production and research situation of blueberry in Hubei Province are introduced.Also, according to the development status, the development trend is analyzed, the development direction is made clear, and the development suggestion is put forward. Related Articles | Metrics

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In vitro Culture and Rapid Propagation of Hedychium flavescens XIONG You-hua, LIU Nian 2011, 40(01): 79-79.  DOI: 10.3969/j.issn.1009-7791.2011.01.020 Abstract ( )   PDF (706KB) ( )   Related Articles | Metrics

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Research Progress on Cold-resistance of Fruit Trees HUANG Min, CHEN Jie-zhong 2011, 40(01): 80-84.  DOI: 10.3969/j.issn.1009-7791.2011.01.021 Abstract ( )   PDF (869KB) ( )   The article reviews the cold-resistance of fruit trees from four aspects, including cells morphology structure, physiological metabolism, germplasm resources and genetic engineering.And the related research work in the future is also involved. Related Articles | Metrics

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Research Advances in AP1 Genes of Plants DING Feng, PENG Hong-xiang, LI Hong-li, ZHU Jian-hua, QIN Xian-quan, SHEN Qing-qing, HE Xin-hua 2011, 40(01): 85-89.  DOI: 10.3969/j.issn.1009-7791.2011.01.022 Abstract ( )   PDF (869KB) ( )   AP1 (APETALA1) genes belong to floral meristem spcific genes and floral organ morphology genes and play an important role in controlling floral meristem identity and floral organ formation.The research progresses of structure, function, expression regulation and evolution relationship of plants' AP1 genes were reviewed and summarized, and their applications on fruit trees were analyzed and outlooked. Related Articles | Metrics

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Uptake and Transport of Boron in Plants and their Genetic Regulations XIONG Xian-yan, LANG Chun-xiu, DU Hai, CHEN Jin-qing, WU Guan-ting 2011, 40(01): 90-94.  DOI: 10.3969/j.issn.1009-7791.2011.01.023 Abstract ( )   PDF (810KB) ( )   Boron is a micronutrient essential for the normal growth and development of plants, and boron deficiency or toxicity is a worldwide agricultural problem, causing the decreases in yield and quality of crops.Since recent years, great progresses have been made in terms of molecular mechanisms of uptake and transport of boron in plants, which lays an important foundation for improvement of tolerances of plants to boron deficiency and toxicity by genetic engineering.In this paper, the progresses in molecular mechanisms and genetic regulations of uptake and transport of boron in plants were reviewed. Related Articles | Metrics