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10 March 2010, Volume 39 Issue 01 Previous Issue    Next Issue

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Analysis of Volatile Components of Flowers from Four Bougainvilleas by GC-MS XU Su-xia, HUANG Qing-yun, LIU Hong-zhou, LIN Chun-song 2010, 39(01): 1-4.  DOI: 10.3969/j.issn.1009-7791.2010.01.001 Abstract ( )   PDF (1425KB) ( )   Bougainvilleas are ornamental,as well as health-friendly.To explore the mechanisms of secondary-metabolism in Bougainvillea,the volatile components of four species of Bougainvillea (Bougainvillea glabra ‘Magnifica’,Bougainvillea ‘Pink Pixie’,Bougainvillea glabra ‘Formosa’ and Bougainvillea×buttiana ‘Mahara’) extracted by distillation were analyzed by GC-MS.Through quantitative analysis and qualitative analysis,85 chemicals were separated.And after searching NIST database,72 chemicals were identified which account for 90% of total.Of them,there are more than one health-friendly chemicals including Phytol,Vitamin E,9,12-Octadecadienoic acid (Z,Z),Eugenol and 9,12,15-Octadecatrienoic acid(Z,Z,Z) in the flowers of Bougainvilleas. Related Articles | Metrics

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Effects of Applied Boron on Boron Forms and Leaf Photosynthesis of Papaya XIE Zhi-nan, LAI Rui-yun, ZHONG Zan-hua, SU Ming-hua 2010, 39(01): 5-8.  DOI: 10.3969/j.issn.1009-7791.2010.01.002 Abstract ( )   PDF (866KB) ( )   Effects of applied boron (soil application of boron,spraying boron on the plant) on the boron forms and leaf photosynthesis of papaya plants were investigated.The results showed that the two methods of boron application both increased the contents of boron forms and the total content of boron in plant leaves,petiole and root,in which the water soluble boron,semi-bound boron and total content of boron in leaves increased significantly.The increase of boron content of plants increased photosynthetic rate in leaves,but excessively high level of boron could inhibit the photosynthetic capacity,and even lead to a significant drop of photosynthesis. Related Articles | Metrics

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Effects of Arbuscular Mycorrhizal Fungus on Absorbing Phosphorus and Excreting Organic Acids of Poncirus trifoliata Seedlings under Low-phosphorus Stress LIU Jin-fa, WANG Peng, LUO Yuan, XIE Ya-chao, WAN Yuan, XIA Ren-xue 2010, 39(01): 9-13.  DOI: 10.3969/j.issn.1009-7791.2010.01.003 Abstract ( )   PDF (924KB) ( )   The effects of arbuscular mycorrhizal fungus (Glomus mosseae and G.versiforme) on the dry weight and absorbing phosphorus of Poncirus trifoliata seedlings and its root excreting organic acids under low-phosphorus stress with the Al-P as phosphorus source and poncirus trifoliata seedlings as experimental material were studied in sand culture and sterilization greenhouse.The results showed that the inoculation of AM not only markedly increased the dry weight of overground part and underground part of Poncirus trifoliata,which raised by 16.79%~135.25%,but also remarkably increased phosphorus uptake with the contribution rate up to 17.04%~71.95% (G.m>G.v).Fertilizing Al-p could observably raise the contribution rate.The kinds of organic acid which were secreted by the root systems of AM fungi inoculation were different from the control.We only detected six acids (oxalic acid,malic acid,lactic acid,acetic acid,maleic acid and citric acid) in non-inoculated treatment materials,while 7 acids (oxalic acid,tartaric acid,malic acid,lactic acid,acetic acid,citric acid,succinic acid) in G.m treatment materials,and also tartaric acid and no maleic acid detected in G.v treatment materials.Inoculation also increased the content of organic acid secretion (increased by 19.80~56.87 mg/kg) of trifoliate root,and the application of AlPO4 greatly increased organic acid content (increased by 20.06~21.84 mg/kg);uninoculated plant root secreted a bit of organic acid;Mycorrhizal poncirus trifoliata seedlings excreted malic acid (42.87%),citric acid (39.22%) and oxalic acid (12.06%). Related Articles | Metrics

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Preliminary Study on the Fruit Set of Cross-Pollination between Cymbidium hybridium and C. sinense ZENG Bi-yu, ZHU Gen-fa, ZHANG Wen-hui 2010, 39(01): 14-16.  DOI: 10.3969/j.issn.1009-7791.2010.01.004 Abstract ( )   PDF (1326KB) ( )   3 cultivars of C. hybridium and 1 cultivar of C. sinense were chosen to self-bred and cross-bred.21 fruits of 4 cross-combinations were attained successfully from 257 pollinated flowers of 10 combinations.The average fruit set was 8.1%.The mean fruit set of reciprocal cross between C. hybridium and C. sinense has little difference.The trends of fruit growth curve of each combinations were in essence the same.The ovaries inflated after pollination 10~12 days and the size of capsule tended to stable between 60 and 80 days.Most of fallen fruits dropped within 30 days after pollination,but some fallen fruits yellowed and dropped in mid-later growing period.The incoordination of parents' genetic material and the cultivation management were important factors to the fruit set. Related Articles | Metrics

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Effect of Artificial Aging on Physiological and Biochemical Characteristics of Wheat Seeds KONG Zhi-you, LIU Ye-ju, QIN Peng 2010, 39(01): 17-20.  DOI: 10.3969/j.issn.1009-7791.2010.01.005 Abstract ( )   PDF (902KB) ( )   A study on the physiological and biochemical changes during storing and appropriate artificial aging conditions of wheat seeds was conducted.Five waxy and non-waxy wheat lines seeds were established during artificial aging under 50℃ and 90% RH for 0,2,4,6 and 8 d,respectively.The results showed that the electrical conductivity increased first and then decreased,and the germination rate decreased gradually with the artificial aging time prolonging.Soluble sugar content of non-waxy wheat seeds decreased,and then increased,but decreased at last during the artificial aging.Soluble sugar content of waxy wheat seeds increased during the artificial aging.SOD,POD and CAT activities decreased lowest in 2 days,but remained unchanged still.Soluble protein content increased in 2 days,and then decreased during artificial aging.MDA content decreased,and then increased,but decreased at last during the artificial aging. Related Articles | Metrics

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Preliminary Study on Photosynthetic Characteristics of Citrus microcarpa in Autumn YOU Yang, ZHOU Xiu-mei, SHEN Xiao-yu, NING Xiao-ming, MENG Feng-li 2010, 39(01): 21-24.  DOI: 10.3969/j.issn.1009-7791.2010.01.006 Abstract ( )   PDF (894KB) ( )   The photosynthetic characteristics of Citrus microcarpa were determined with the Li-6400 Photosynthetic system.The results showed that the diurnal variation of net photosynthetic rate (Pn) in Citrus microcarpa was a double-peak curve and there also exists a clear depression at noon.The first peak occurred at 11:00,the second one at 14:00.The maximum of net photosynthetic rate (Pmax) was 3.4467 μmol/m2·s.Correlation analysis showed that there were positive relationship between Pn and transpiration rate (Tr) and stomatal conductance (Gs),and negative relationship between Pn and internal CO2 concentration (Ci).Under the concentration of CO2 400 μmol/mol,the light compensation point (LCP) of Citrus microcarpa was 67 μmol/m2·s,and light saturation point (LSP) was 672 μmol/m2·s.In sum,Citrus microcarpa is a typical masculine and shade-resistant plant which has strong adaptability to the circumstance. Related Articles | Metrics

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Seasonal Changes of Photosynthetic Characteristics of Houses Ornamental Plant Osmanthus fragrans var. semperflorens SHI Jian-yu 2010, 39(01): 25-28.  DOI: 10.3969/j.issn.1009-7791.2010.01.007 Abstract ( )   PDF (929KB) ( )   The seasonal changes of light response curve and photosynthetic characteristics of Osmanthus fragrans var.semperflorens were determined by LI-6400.The results showed that the light response curve changes of O. fragrans var.semperflorens's leaves at different seasons were similar.The net photosynthetic rate and light compensation point were Jan< Apr< Oct< Jul,and the light saturation point was Jul< Oct< Apr< Jan.The day changes of Pn,Tr,VPD and Gs of O. fragrans var.semperflorens were single peak curve separately at January and April,while double peak curve and had photosynthetic "lunch-break" atmosphere at July and October.The seasonal change of Ci was that Ci decreased continuously at January,while decreased first and then reached a small peak value at 15:00 at April,and appeared the lowest point at 10:00 and the highest point at 15:00 at July and October. Related Articles | Metrics

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The Changes and Relationship of Leaf SPAD Value, N Nutrition and Agronomic Traits in Leaves of Rubber Tree Seedlings under Low-N Stress ZHOU Jun, LIN Wei-fu, WANG Jun, CAO Jian-hua, CAI Ming-dao 2010, 39(01): 29-33,44.  DOI: 10.3969/j.issn.1009-7791.2010.01.008 Abstract ( )   PDF (1253KB) ( )   Seedlings of three varieties (GT1,RRIM600,PR107) of Hevea brasiliensis were treated with low N (0%N,50%N,100%N) stress and the changes and their relationship of the leaf SPAD values,N nutrition and agronomic traits were studied.The results showed that:(1) low N stress significantly decreased the leaf SPAD value,leaf nitrogen content (LNC),nitrate reductase activity (NRA) and reduced distance between leaf-umbrella (DBL),leaf area (LA).And these impact aggrandized as the stress intensity increased.(2) Responses to low-N stress were in different degrees between different varieties of rubber tree seedlings.On the whole,GT1 was most sensitive to stress,PR107 next,and RRIM600 was the least sensitive.(3) The SPAD value,DBL,LA were significantly positive correlated with LNC,NRA in Hevea brasiliensis seedling leaves.Therefore,leaf SPAD value,DBL and LA would be shortcut measures to evaluate the nitrogen nutrient condition of rubber plant. Related Articles | Metrics

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Comparative Anatomy of Vegetative Organs of Dioscorea zingiberensis in Different Ploidies ZHU Yan, XU Zeng-lai, WANG Qiong, ZHAO Meng 2010, 39(01): 34-39.  DOI: 10.3969/j.issn.1009-7791.2010.01.009 Abstract ( )   PDF (1349KB) ( )   To provide attestation for cultivation of good germplasm of Dioscorea zingiberensis,the anatomic structure of vegetative organs of three ploidies D. zingiberensis,diploid (2x=20),triploid (3x=30) and tetraploid (4x=40) were comparably studied by paraffin method.The results showed that the structural differences among three ploidies of D. zingiberensis were leaf thickness,palisade tissue thickness,P/S,P/L,VR and periderm thickness.The triploid D. zingiberensis had the largest palisade tissue thickness,P/S,P/L and VR,while the tetraploid had the largest leaf thickness and periderm thickness.The multiploid put up stronger predominance than diploid as a whole. Related Articles | Metrics

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Preliminary Studies on Two-dimensional Electrophoresis for Proteome of Phalaenopsis Leaves CHEN Yi-liang 2010, 39(01): 40-44.  DOI: 10.3969/j.issn.1009-7791.2010.01.010 Abstract ( )   PDF (2905KB) ( )   Phalaenopsis leaves keep less protein and more non-protein substances like pigment,phenol and so on.To minimize the interference by non-protein substances,a 2-DE (two-dimensional electrophoresis) method which suited for the separation of protein from Phalaenopsis leaves was established in this experiment,on the basis of the total-protein extraction method and the silver dyeing method were modified and improved and the influential factors of 2-DE were compared and selected. Related Articles | Metrics

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Tissue Culture and Establishment of Regeneration System for Cynanchum paniculatum ZHANG Song-yan, GAO Yang, CHEN Yang-sheng, LI Tie-song, JIANG Chang-yang 2010, 39(01): 45-48.  DOI: 10.3969/j.issn.1009-7791.2010.01.011 Abstract ( )   PDF (868KB) ( )   The induction and differentiation were studied on the callus of cotyledon of Cynanchum paniculatum and rooting and transplanting of test-tube plantlets as well as establishment of asexual system of Cynanchum paniculatum were successfully carried out in the experiment.The results suggested:MS+6-BA 0.3 mg/L+NAA 0.2 mg/L+2,4-D 0.4 mg/L was the optimum medium for callus induction and subculture of cotyledon.The ideal medium for callus differentiation was MS+6-BA 0.8 mg/L+NAA 0.2 mg/L.The medium of MS+6-BA 0.6 mg/L+NAA 0.2 mg/L was optimal for differentiation and subculture of adventitious buds.And the cider was used as ideal matrix for plantlets culture with vigorous and variant characters.Based on the result of this experiment,induction and regeneration system of Cynanchum paniculatum could be set up. Related Articles | Metrics

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Effects of Copper Sulfate Concentration and Injury on Leaves' Color Keeping DU Fang, WANG Duo, PAN Wen-ting 2010, 39(01): 49-52.  DOI: 10.3969/j.issn.1009-7791.2010.01.012 Abstract ( )   PDF (875KB) ( )   In this paper,15 kinds of green leaves were treated with 0%,1.02%,5.85%,10.22%,14.21% and 17.15% copper sulfate solution at 90℃,and leaves which were uneasy to be kept in color were injured to study the effects of copper sulfate concentration and injury on leaves' color keeping.The results showed that different kinds of leaves responded differently to copper sulfate solution.Heat copper sulfate solution deformed paper leaves (e.g.Oxalis corymbosa) easily and had no effect on some kinds of leathery leaves (e.g.Yucca elephantipes and Cycas revoluta).Young leaves' color was much easier to be kept than that of matured leaves.Copper sulfate concentration had no effect on color,but had effect on the time of color keeping.The higher the copper sulfate concentration,the shorter the time of returning green.The time of returning green was the same in 14.21% and 17.15% copper sulfate concentration.Leaves' color being treated by copper sulfate changed between light and dark before and after dried.A few leaves,such as Chaenomeles speciosa,could keep original color after dried naturally without being treated by copper sulfate.And injury was favor to color keeping of some kinds of leathery leaves. Related Articles | Metrics

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Preliminary Research on Resource Investigation and Classification of Bombax Ceiba in Hainan Province WANG Jian, SHUI Qing-yan, SHI Jing, ZHANG Xuan-bing 2010, 39(01): 53-56.  DOI: 10.3969/j.issn.1009-7791.2010.01.013 Abstract ( )   PDF (872KB) ( )   The investigation and analysis were carried out on the distribution and characters of Bombax ceiba in Hainan Province,and a preliminary classification standard was formed.According to the investigation,Bombax ceiba possessed rich diversity in ornamental characters and enormous potential in selective breeding.The results of principal component analysis and cluster analysis showed that the size of flower should be set as the first standard of classification,and the flower color as the second one and the bifurcation height of stamen as the third one. Related Articles | Metrics

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Analysis on the Orchidaceae Flora of the Sanqingshan Mt. in Jiangxi Province ZANG Min, LI Yong-fei, QIU Xiao-lan, QI Li-ping 2010, 39(01): 57-62,70.  DOI: 10.3969/j.issn.1009-7791.2010.01.014 Abstract ( )   PDF (978KB) ( )   The Sanqingshan Mt.locates in the east of Huaiyu Range in Jiangxi Province which situates between 28°52'~28°57'N latitude and 118°00'~118°06'E longitude.There are abundant resources of Orchidaceae including 30 species of 21 genera in Sanqingshan,which account for genera 56.76% and species 38.46% of Orchidaceae in Jiangxi Province,and 12.28% and 2.41% in China respectively.The Orchidaceae flora owns the features that the main life form is ground-living orchid,the main areal types is temperate class,and floristic elements scatter and lack endemic species.The comparison of Orchidaceae flora generic Areal types and comparability among Huangshan Mt.,Wuyishan Mt.,Tianmushan Mt.,Jinggangshan Mt.,Lushan Mt.and Sanqingshan Mt.are analyzed,and the conclusions are as follows:the generic comparability coefficient is higher than species,and the contact of flora among 6 Mt.is tight;North temperate is primary generic areal type in Lushan,Tianmushan,Huangshan and Sanqingshan;Tropical Asia to Tropical Oceania is primary generic areal type in Jinggangshan and Wuyishan;East Asia and Endemic to China is primary species areal type in 6 Mt..According to the ecotypes,Terrestrial orchid is primary and Epiphytic orchid secondary.There are only two ecotypes in Sanqingshan and Huangshan,but all ecotypes appear in Wuyishan,Tianmushan,Jinggangshan and Lushan.Base on China Species Red List,all endangered plants in 6 Mt.are concluded at last. Related Articles | Metrics

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Studies on Bambusoideae and Its Flora in Yong'an, Fujian Province LIN Min-shui 2010, 39(01): 63-68.  DOI: 10.3969/j.issn.1009-7791.2010.01.015 Abstract ( )   PDF (907KB) ( )   According to investigation,there exists a total of 63 species and varieties falling into 11 genera of Bambusoideae which are natural distribution in Yong'an City,Fujian Province.The genera and species of Bambusoideae respectively account for 28.2% and 11.4% of China,and 64.7% and 48.8% of Fujian Province.Among them,4 species of Sinobambusa rubroligula,Indocalamus hunanensis,Pseudosasa maculifera var.hirsuta and Phyllostachys heterocycla cv.Luteosulcata are the new records of bamboo distribution in Fujian.By the analysis of types,the major bamboo plants in Yong'an City are mainly monopodial-scattered and sympodial-mixed species.Yong'an is the distributive center of cluster bamboo and mixed bamboo which also have an obviously transitive characteristic from cluster bamboo to seattered bamboo.Floristic analysis indicated that there are 5 distributive types of Bambusoideae in Yong'an (contains a sub-type),and the East Asia holds the first position. Related Articles | Metrics

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New Discovery of Yoania japonica Maxim. in Baishanzu Nature Reserve, Zhejiang Province and Its Floristic Significance ZHU Hui-ling, ZHANG Yong-hua, HU Ling-zhi, DING Bing-yang 2010, 39(01): 69-70.  DOI: 10.3969/j.issn.1009-7791.2010.01.016 Abstract ( )   PDF (823KB) ( )   Firstly report the distribution of Yoania japonica Maxim.in Baishanzu Nature Reserve of Zhejiang province and its floristic significance was described. Related Articles | Metrics

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The Application of Ornamental Bamboos for Garden Landscaping in Xiamen, Fujian Province WANG Hong-hua 2010, 39(01): 71-74.  DOI: 10.3969/j.issn.1009-7791.2010.01.017 Abstract ( )   PDF (1950KB) ( )   On the basis of the investigation of bamboo resources in parks and green areas of Xiamen,31 varieties ornamental bamboos were chosen to analyze their ornamental features,then the application in modern landscape greening was expounded. Related Articles | Metrics

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The Category of Color-Leafed Plants and Their Application in Quanzhou, Fujian CHEN Yin-zhu 2010, 39(01): 75-80.  DOI: 10.3969/j.issn.1009-7791.2010.01.018 Abstract ( )   PDF (2827KB) ( )   The color-leafed plants play an important role in landscaping for their rich color and great ornamental value.The category of color-leafed plants and their application status in Quanzhou are studied.Some suggestions for introduction and application are also put forward. Related Articles | Metrics

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Tissue Culture of Primula denticulata subsp. sinodenticulata XIE Wei-jia, LI Shi-feng, LI Han, JIANG Ya-lian, CAI Yan-fei, LI Shu-fa 2010, 39(01): 81-81.  DOI: 10.3969/j.issn.1009-7791.2010.01.019 Abstract ( )   PDF (766KB) ( )   Related Articles | Metrics

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Application of Near Infrared Spectra (NIRS) Technology in Prediction of Maturity Stage of Fruit MAO Sha-sha, ZENG Ming, HE Shao-lan, ZHENG Yong-qiang, YI Shi-lai, DENG Lie 2010, 39(01): 82-87,96.  DOI: 10.3969/j.issn.1009-7791.2010.01.020 Abstract ( )   PDF (962KB) ( )   Near infrared spectroscopy has become very popular in food industry because of many important practical advantages such as fast,accurate and simultaneous multicomponent analysis.In recent years,more and more attention is paid to applying NIRS technology to fruit development,prediction of maturity stage and internal quality measurement.In this article,not only the fundamental principles and characteristics of near infrared spectroscopy were summarized,but also the status quo and existing problems in prediction of mature stage were presented.The research direction about applying NIRS technology in food industry is provided for us. Related Articles | Metrics

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Research on Auxin Influx Transporter AUX1 of Arabidopsis ZHANG Bi-yu, SU Liang-chen, SUN Xu-zhuo 2010, 39(01): 88-91.  DOI: 10.3969/j.issn.1009-7791.2010.01.021 Abstract ( )   PDF (983KB) ( )   Research on Auxin influx transporter AUX1 of Arabidopsis was summarized in this paper.A method which could directly determinate the appetency of homologous ligand was mainly introduced,and the initial interaction between plant transporter and its hormone was also described. Related Articles | Metrics

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Progress on Anti-Virus Genetic Engineering of Orchid MING Yan-lin, ZHENG Jin-long, ZHENG Guo-hua, ZHENG Zhi-zhong 2010, 39(01): 92-96.  DOI: 10.3969/j.issn.1009-7791.2010.01.022 Abstract ( )   PDF (900KB) ( )   The orchid virus disease seriously affects the development of orchid industry,research and exploration of orchid virus diseases prevention and control of new technologies,new ways to many researchers have become the focus of widespread concern.In this paper,the current anti-virus research in orchids of various anti-virus applications of genetic engineering strategies is summarized,which includes possible sources of the virus coat protein gene,the movement protein gene and gene-mediated resistance strategy,RNA-mediated anti-viral strategies,plants themselves anti-viral gene-mediated resistance strategy,multigene-mediated resistance strategy,and the use of antibody-mediated resistance strategy and so on.Finally,prospect in the development and application of orchid anti-virus genetic engineering is put forward. Related Articles | Metrics