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10 September 2010, Volume 39 Issue 03 Previous Issue    Next Issue

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Absorption and Accumulation of Cadmium and Lead in Passionfruit XU Wen-bao, ZENG Xin-ping, CAI Xiao-dong, Lin Fu-cong 2010, 39(03): 1-3.  DOI: 10.3969/j.issn.1009-7791.2010.03.001 Abstract ( )   PDF (749KB) ( )   The variation of cadmium (Cd) and lead (Pb) contents on the basis of Cd and Pb applied to soil was investigated in pot-cultured passionfruit (P. edulis Sims and P. edulis Sims f. flavicarpa Degener).The results showed that the distribution of Cd and Pb in passionfruit ranked as:branches >leaves >fruits.The Cd contents had no significant difference in different varieties of passionfruit, while the Pb contents had.The contents of Cd and Pb in the branches and leaves of passionfruit increased with the increasing of their treatment concentration.The contents of Cd and Pb in the branches and leaves of passionfruit had great significant positive correlation with their concentration applied to soil.The passionfruit plant had stronger ability to uptake Cd than Pb with the bio-enrichment coefficients of Cd were all more than 1.When Pb added to soil was 1000 mg/kg, the content of Pb in fruit was less than the maximum limit in contamination-free fruits.In planting practice, even in the higher levels of Pb polluted soil, passionfruit fruit could be free of Pb pollution, but more special attention should be paid to Cd pollution risks. Related Articles | Metrics

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Study on Soil Respiration of Two Forest Types in North Subtropical Zone WANG Feng-wen, YANG Shu-yun, XU Xiao-niu, ZHANG Qing-guo 2010, 39(03): 4-7.  DOI: 10.3969/j.issn.1009-7791.2010.03.002 Abstract ( )   PDF (787KB) ( )   The soil respiration rate of two forest types in Dabieshan Mountain in the western of Anhui Province was statistically analyzed.The results showed that the soil respiration rate of two forest types increased first and then decreased in different seasons.There existed great correlation between soil respiration rate and soil temperature at the depth of 5 cm and 10 cm.There was also highly significant correlation between soil respiration rate and soil organic matter content at the depth of 5~10 cm layer in Chinese-fir forest with the correlation coefficient was 0.978 (p<0.01).In bast oak forest, the soil respiration rate was significantly correlated with soil organic matter content in 0~5 cm layer (0.928, p<0.05). Related Articles | Metrics

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Correlation Research of Carbohydrate Change in Buds and Leaves and Flower Bud Differentiation of Dimocarpus longan ‘Sijihua’ CAI Zhong-fang, PENG Jian, LI Yong-hong, ZHAO Chang-zeng, CHEN Bai-hong, ZHANG Shao-ai 2010, 39(03): 8-11.  DOI: 10.3969/j.issn.1009-7791.2010.03.003 Abstract ( )   PDF (849KB) ( )   To research correlation of carbohydrate content changing in buds and leaves and longan's flower bud differentiation, five to six-year-old ‘sijihua’ longan's autumn shoots were used as the experimental materials. The morphological differentiation process of the flower bud differentiation was observed, flower bud differentiation rate of every period was calculated, the contents changing of total soluble sugar, sucrose, fructose, reducing sugar and starch of buds and leaves were determined and the correlation between the carbohydrate content of leaves and buds and flower bud differentiation rate during the differentiation process was counted. The results showed that, during the differentiation process of ‘sijihua’ longan's autumn shoots, the flower bud differentiation rate reached the highest at main axle of inflorescence differentiation period. The carbohydrate content of leaves and buds showed significant difference in every differentiation period (P≤0.05). The changing trend of starch content in leaves was reduced markedly, while in contrary for buds, which reflected the relationship of the buds and leaves was sink and source respectively during the flower bud differentiation．The correlation of the changing magnitude of total soluble sugar, sucrose and reducing sugar in buds and the flower bud differentiation process was higher compared with leaves, so the measurements of carbohydrate in buds were able to reflect the real estate of the flower bud differentiation all the more, but the related question was still worth researching deeply. Related Articles | Metrics

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Preliminary Study on Embryo Rescue of Interspecific Hybrids between Cymbidium ZENG Bi-yu, XU Chuan-jun, XU Su-xia, ZHANG Wen-hui, HUANG Qing-yun 2010, 39(03): 12-14.  DOI: 10.3969/j.issn.1009-7791.2010.03.004 Abstract ( )   PDF (751KB) ( )   Interspecific hybridization was carried out with Cymbidium hybridium as female parent and C. sinense as male parent.Embryo rescue technique of hybrids was also studied.The results showed that ovaries culture was better than ovules culture, and ovaries culture had become to be effective means of embryo rescue.The germination and growth had significant difference at different growth stages and different culture medium.Ovaries could be cultured in vitro successfully as early as 60d after pollination.However, the germination rate was the highest when ovaries cultured for 90d after pollination, which reached up to 80%.Of the media studied, VW+BA 0.2 mg/L+NAA 0.2 mg/L+peptone 3 g/L+cascin hydrolyaate 500 mg/L+coconut milk 10% was the optimal medium for ovaries culture. Related Articles | Metrics

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Salicylic acid-induced Salt Resistance and its Mechanism in Chinese Cabbage Seedlings XU Fen-fen, YE Li-min, ZHANG Yong, WANG Ai-bin, LIN Guo-wei 2010, 39(03): 15-17.  DOI: 10.3969/j.issn.1009-7791.2010.03.005 Abstract ( )   PDF (814KB) ( )   The experiments were performed to investigate the effects of exogenous SA on the physiological parameters in Chinese cabbage under NaCl stress.Chinese cabbage seedlings were treated by foliar spray of 0, 0.1, 0.5, 1.0, 1.5 mmol/L SA, and were then subjected to NaCl stress.Results indicated that 0.5 mmol/L SA significantly improved the plant height, plant dry weight, root thickness, leaf area and significantly reduced the leaf malondialdehyde (MDA) content and electrolyte leakage rate, significantly enhanced superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and other plant cell protective enzyme activity of leaf.Appropriate concentration of exogenous SA could reduce the stress on the cabbage injuries by increasing plant osmotic adjustment ability, antioxidant capacity and protecting the membrane structure.The best treatment concentration of SA was 0.5 mmol/L. Related Articles | Metrics

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Resorption Efficiencies of Nitrogen and Phosphorus of Phyllodes during Senescence for Acacia confusa at Wulaofeng,Xiamen TAN Zhong-qi 2010, 39(03): 18-20,78.  DOI: 10.3969/j.issn.1009-7791.2010.03.006 Abstract ( )   PDF (811KB) ( )   Nitrogen and phosphorus concentrations, N:P ratio and nutrient resorption efficiency of phyllodes during senescence for Acacia confusa were studied at Wulaofeng, Xiamen.The results showed:(1) Mature phyllodes had the high N concentration, with 39.90±2.10 mg/g in winter, and 43.13±1.67 mg/g in spring;P concentration of mature phyllodes was 1.24±0.04 mg/g in winter, and 1.16±0.05 mg/g in spring, respectively;N and P concentrations decreased with phyllodes senescence.(2) N:P ratio of mature phyllodes was 32.26±1.53 in winter and 37.37±1.61 in spring, respectively;N:P ratios were above 16, which illustrated that Acacia confusa forest was P-limited.Resorption efficiency of P was significantly higher than resorption efficiency of N. Related Articles | Metrics

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The Diagnosis of the Ancient Trees of Ficus microcarpa CHI Min-jie, LIU Hai-sang, YOU Si-yang, CHEN Qing-zhi, LIN-xi, WANG Wen-qing 2010, 39(03): 21-23.  DOI: 10.3969/j.issn.1009-7791.2010.03.007 Abstract ( )   PDF (819KB) ( )   Based on the general investigation and analysis of the ancient trees of Ficus microcarpa at Gulangyu by use of the growth indices and the physiological indices, such as the growth status of annual branches, leaves and fruits and the chlorophyll fluorescence, the diagnosis of the ancient trees of Ficus microcarpa with different growth vigor was discussed to provide evidence for their maintenance and rejuvenation.The results indicated that the growth indices and the physiological indices could consistently instruct the health status of the ancient trees of Ficus microcarpa.Thus the diagnosis by the combination of the growth indices and the physiological indices could be applied to the ancient trees of Ficus microcarpa. Related Articles | Metrics

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Preliminary Study on Stem Disinfection of Zygocactus truncactus and Its Multiple Bud Proliferation and Rooting YIN Ming-hua, DAI Qin-qin, ZHANG Jie, LIU Lin-mei, WU Shu-ying 2010, 39(03): 24-26.  DOI: 10.3969/j.issn.1009-7791.2010.03.008 Abstract ( )   PDF (984KB) ( )   The establishment of sterile system and the effect of plant growth regulators on multiple bud proliferation and rooting were studied by tissue culture and single-factor experimental method, using Zygocactus truncactus stems as material.The results showed that the best sterilization method of Z. truncactus stems was firstly treated with 75% alcohol for 30s and then treated with 0.1% HgCl2 for 10min, the best multiple bud proliferation medium of Z. truncactus was MS+KT 4 mg/L+NAA 0.1 mg/L, and the best rooting medium was 1/2 MS+NAA 0.5 mg/L. Related Articles | Metrics

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The Construction of One-Step Rapid Propagation System of Anemarrhena asphodeloides HONG Sen-rong, CHENG Xi-yun, ZHOU Lu, YANG Xiao-lu, ZHOU Fen 2010, 39(03): 27-29.  DOI: 10.3969/j.issn.1009-7791.2010.03.009 Abstract ( )   PDF (1114KB) ( )   One-step rapid propagation system of Anemarrhena asphodeloides was studied with its tillers as experimental material.The results showed that the best direct regeneration medium of A. asphodeloides tiller was MS+KT 1 mg/L+NAA 0.5 mg/L, and the best callus induction and regeneration medium of A. asphodeloides tiller was MS+KT 2 mg/L+NAA 0.5 mg/L.The one-step rapid propagation system of A. asphodeloides tiller was built in this experiment, which would provide theoretical and technical basis for the factory production of A. asphodeloides plantlets. Related Articles | Metrics

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Callus Induction of Swertia mileensis and Its Subculture in Liquid ZHEN Li 2010, 39(03): 30-31.  DOI: 10.3969/j.issn.1009-7791.2010.03.010 Abstract ( )   PDF (709KB) ( )   The young leaves of Swertia mileensis, as explants, were cultured on MS culture medium by adding different kinds and levels of hormones to study their callus induction.The results showed that the most suitable phytohormone combination to induce callus were IAA 1.0 mg/L+NAA 1.0 mg/L+KT 1.0 mg/L and NAA 4.0 mg/L+KT 2.0 mg/L+GA3 1.0 mg/L, with the corresponding induction rate were 96.6% and 96.0% respectively.The callus subculture in liquid by adding the two kinds of phytohormone combination could lead the proliferation rate to 257.3% and 310.2% 10 days later. Related Articles | Metrics

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Numerical Taxonomy and Principal Component Analysis of Lycoris from China YUAN Ju-hong 2010, 39(03): 32-37.  DOI: 10.3969/j.issn.1009-7791.2010.03.011 Abstract ( )   PDF (956KB) ( )   Based on the field observation and the data collected from references, cluster analysis and principal component analysis (PCA) for 35 characters of 13 species and 2 varieties of Lycoris from China were conducted.The results showed that 13 species and 2 varieties of Lycoris were classified into 8 subgroups and 2 groups based on Q cluster analysis, which basically agreed with the traditional taxonomic studies, and the hybrid origin viewpoint of L. albiflora, L. houdyshelii, L. straminea, L. rosea and L. haywardii was also supported.While L. anhuiensis as an independent species was not supported, it should be recognized as a variety of L. longituba due to their very close relationships.35 characters were divided into 7 groups according to R cluster analysis.The principal component analysis showed that 35 characters were intergrated into 5 principal components (PC) and their additive contributing rate amounted to 82.55%.16 major discriminating characters were selected according to the relativity between 5 PC and characters.Among them, the characters of bulb shape, lobe width, and the ratio of stamen length:lobes length may be used for division major group of Lycoris, and the lobes with stripes or not, filament color, perianth color, flowering stem diameter, apex and margin color of young leaves, and seeds or not may be the foundation of divided species. Related Articles | Metrics

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Comparative Anatomy of the Leaves of Amomum (Zingiberaceae) TANG Yuan-jiang, LIAO Jing-ping, WU Qi-gen 2010, 39(03): 38-43.  DOI: 10.3969/j.issn.1009-7791.2010.03.012 Abstract ( )   PDF (1764KB) ( )   Comparative anatomy on the leaf transverse section of 12 species belonging to Amomum was studied by paraffin method.The results showed that the epidermis consists of one layer of epidermal cells.Epidermal cells of adaxial side are usually wide and tall in species with no hypodermis, while epidermis was thin with cell wall thickening in species with hypodermis.The structure of hypodermis kept diversity in different taxa.Always, palisade cells in mesophyll consist of 1-2 layers and spongy cells of 2-6 layers.Moreover, tannin occurs commonly in mesophyll and crystals are also present in most species examined.The morphology of extreme vascular bundle at midrib phloem is various, but the vascular bundle system II is generally absent, and no species examined appears all four systems at the same time.Finally, the morphological characteristics of leaf margin were synoptically described.In addition, based on the diagnostic characters and the previous research, the systematic significance of the anatomical structure of leaves was also discussed in this paper. Related Articles | Metrics

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Survey and Analysis of Invasive Plants in Riparian zone of Gan River LEI Ping, GE Gang, CHEN Shao-feng, LIU Yi-zhen, XU Yan-hua 2010, 39(03): 44-48.  DOI: 10.3969/j.issn.1009-7791.2010.03.013 Abstract ( )   PDF (821KB) ( )   Survey on invasive plants in riparian zone of Gan River was conducted on the basis of three aspects of river basin, vegetation community and interference manners.There exists 31 species of invasive plants belonging to 27 genera in 17 families.Among them, Compositae is the most consisting of 9 species.There is not significant difference in the dominance of invasive plants among up-, mid- and downstream of riparian zone in Gan River.However, the distribution of invasive plants has been significantly effected by vegetation types in the riparian, e.g., the dominances of invasive plants in herbosa and shrub are significantly greater than in high-forest community.The interference positively impacted the distribution of invasion plants;the significant difference for the dominance of invasive plants was observed in with and without inference, but no in different inference manners. Related Articles | Metrics

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Analysis of Geographical Elements of Machilus versicolora Community in Mangdangshan Nature Reserve,Fujian Province HUANG Zi-liang, CHEN Xue-fu 2010, 39(03): 49-54.  DOI: 10.3969/j.issn.1009-7791.2010.03.014 Abstract ( )   PDF (913KB) ( )   With investigation and analysis of the geographical elements of Machilus versicolora community in Mangdangshan Nature Reserve of Fujian province, the results show that there are 195 species of seed plants in 134 genera and 74 families belonging to 9 families and 13 genera of areal types.The community is dominated obviously by tropical geographical elements with 86 genera and 45 families, respectively 80.4% and 68.8% out of the family and genera of total seed plants.Among them, pantropical distribution (33 families, 32 genera) dominates in geographical elements with 58.9% of families and 25.6% of genera.Temperate distribution includes 11 families and 37 genera, respectively occupying 19.6 percent and 29.6 percent.The community put up those features that species are rich, geographical elements are complex, tropical elements are obvious, temperate elements are plentiful, the origin of flora is ancient and also existing lots of endemic elements. Related Articles | Metrics

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Floristic Analysis on Vascular Plants of Sanqingshan Mountain in Jiangxi Province ZANG Min, HUANG Li-fa 2010, 39(03): 55-62.  DOI: 10.3969/j.issn.1009-7791.2010.03.015 Abstract ( )   PDF (4042KB) ( )   The Sanqingshan Mountain locates in the east of Huaiyu Ranges, which is in the northeast of Jiangxi Province.There are various species of vascular plants in Sanqingshan with 1915 species (involving variants, subspecies and forma) belonging to 878 genera of 193 families.Based on the investigation and statistical analyses of distribution types, for families, the pantropical distribution is the most of 34.2%, the cosmopolitan distribution is 31.09% and the north temperate zone is 15.03%.Temperate elements are less than tropical elements with the ratio of 41:90.And for genera, the north temperate zone is 17.88%, the pantropic type is 14.81% and the East Asia type 12.76% at generic level, with the temperate elements are slightly more than the tropical elements (427:341).At species level, the Endemic to China type reaches 48.92%, the East Asia type 30.73%, and the tropical Asia type 6.73%, and the temperate elements are much more than the tropical elements (705:245).The specific areal types indicate that the floristic characteristic of the reserve is obviously subtropical montane nature.According to the regionalization of Chinese flora, the authors propose that the Endemic to China type can be divided into 12 subtypes, of which the subtype of East-, Central- and Southwest-(South-) China takes the biggest proportion.It reflects the relationships among the floristic elements mentioned above. Related Articles | Metrics

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Ecological Distribution of Pteridophyte in Tongledashan Nature Reserve XU Yong-fu, YU Xun-lin, ZHU Zhi-ping 2010, 39(03): 63-66.  DOI: 10.3969/j.issn.1009-7791.2010.03.016 Abstract ( )   PDF (845KB) ( )   Based on the basic composition of Pteridophyte in Tongledashan Nature Reserve, the ecological characteristics and ecological distribution of Pteridophyte were studied systematically in present paper.The research shows that the ecological distribution of Pteridophyte in the area has the following characteristics:(1) Ancient Pteridophyte are widely distributed;(2) The vertical distribution of Pteridophyte is relatively obvious;(3) Pteridophyte has significant advantage in understory vegetation;(4) There are a few national key protected Pteridophyte;(5) The sensitive of Pteridophyte has strong indication. Related Articles | Metrics

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The Landscape Construction and Plant Design of Rain Forest Area in Xiamen Botanical Garden WENG Ping, CHEN Heng-bin 2010, 39(03): 67-71.  DOI: 10.3969/j.issn.1009-7791.2010.03.017 Abstract ( )   PDF (815KB) ( )   The landscape construction and plant design of rain forest area in Xiamen Botanical Garden are introduced in this paper.The organic combination of primary vegetation, landscape facilities and plants with tropical rain forest feature constructs characteristic subtropical rain forest scenery. Related Articles | Metrics

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Studies on Relationship between Altitude and Yield of Castanea mollissima in Mountainous Area of Northeast Fujian WEi Pu-mao 2010, 39(03): 72-74.  DOI: 10.3969/j.issn.1009-7791.2010.03.018 Abstract ( )   PDF (747KB) ( )   The relationship between altitude and yield of Castanea mollissima in mountainous area of Northeast Fujian was studied by randomized block design in this experiment.According to the comparative analysis, the result showed that there was significant difference in the fruit buds number and the field of Castanea mollissima among different altitude, but there was not proportion relation between altitude and yield of Castanea mollissima.The suitable altitude for planting Castanea mollissima was 400~800 m, which could bring high yield and good economic benefit. Related Articles | Metrics

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Rare and Precious Southern Medicinal Plants in Fujian Province LIU Yu-mei 2010, 39(03): 75-78.  DOI: 10.3969/j.issn.1009-7791.2010.03.019 Abstract ( )   PDF (781KB) ( )   Based on the practical experience on the introduction, planting and domestication of southern medicinal plants, 35 species of southern medicinal plants are summarized in this paper, and their families, Latin names, medicinal organs, tastes, habitats are also edited and recorded.Moreover, the suggestions about the rational exploitation and sustainable development of southern medicinal plants are put forward. Related Articles | Metrics

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Tissue Culture and Rapid Propagation of Changnienia amoena GAO Li, YANG Bo, LI Hong-lin 2010, 39(03): 79-79.  DOI: 10.3969/j.issn.1009-7791.2010.03.020 Abstract ( )   PDF (679KB) ( )   Related Articles | Metrics

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Studies on the Control of Contamination Rate in Tissue Culture of Anthurium andraeanum SHI Qun 2010, 39(03): 80-81.  DOI: 10.3969/j.issn.1009-7791.2010.03.021 Abstract ( )   PDF (709KB) ( )   Related Articles | Metrics

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Progress in Effects of Light,Saccharide and Hormones on the Anthocyanin Synthesis and Accumulation in Plants CHENG Hai-yan, LI De-hong 2010, 39(03): 82-86.  DOI: 10.3969/j.issn.1009-7791.2010.03.022 Abstract ( )   PDF (903KB) ( )   Anthocyanin exists in the vacuoles of some leaves, flowers, fruits, seed epidermis and seed coat and is a water-soluble pigment that causes these organs showing color from red to black and others.The process of anthocyanin synthesis not only be regulated by gene, but also be affected by many other different factors.First, light regulates related enzyme gene expression directly or indirectly by signal transduction pathway.Second, sugar often coordinated controls pigment in flower altogether with light.Finally, hormone is also an important factor affecting the synthesis of anthocyanin.It often influences the metabolic process and gene expression in plants to affect anthocyanin synthesis and accumulation.Research progress in recent 20 years was also reviewed. Related Articles | Metrics

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Research Progress on Virus Detection Techniques in Orchid LIN Zhi-kai, GUO Ying, LIU Li-qing 2010, 39(03): 87-92.  DOI: 10.3969/j.issn.1009-7791.2010.03.023 Abstract ( )   PDF (843KB) ( )   The research progress of virus detection techniques in orchid was reviewed, and the application of serological detection assay and molecular biological detection assay was also introduced in detail.Moreover, the merit and demerit of some detection assays were summarized, which afforded reference for the establishment of convenient detection techniques in orchid industry. Related Articles | Metrics

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Transposons Superfamily of Higher Plant and Its Application LI Hong, HUANG Xiao-tian 2010, 39(03): 93-96.  DOI: 10.3969/j.issn.1009-7791.2010.03.024 Abstract ( )   PDF (800KB) ( )   Transposons are mobile DNA molecules existing in genomes of many organisms.The transposons superfamilies of higher plants were introduced including LTR retrotransposons, hAT, CACTA elements, Mutator and MULEs, Tcl/mariner, miniature inverted repeat transposons MITEs and so on.Moreover, the transposon tags systems of plants and their selection methods, the application of transposons on the analysis of diversity and genetic linkages, the study of plant genomics and the modification of plant characters were also summarized in this paper. Related Articles | Metrics