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Analysis of Genetic Diversity and Inter-specific Relationships of Lycoris (Amaryllidaceae) from China by SSR-PCR YUAN Ju-hong 2011, 40(04): 1-7.  DOI: 10.3969/j.issn.1009-7791.2011.04.001 Abstract ( )   PDF (516KB) ( )   Genetic diversity and inter-specific relationships of Lycoris were analyzed by SSR-PCR technique. 46 SSR primer pairs were employed to amplify genomic DNA of Lycoris. 18 out of 46 SSR primer pairs which showed high polymorphism were used to detect genetic diversity and inter-specific relationships among 15 Lycoris species and Narcissus tazaetta var. chinensis as an outgroup in this study. The results indicated that the genetic diversity is high, and their percentage of polymorphic bands is up to 97.63% using the non-denaturing polyacrylamide gel electrophoresis and modified argentation. The 20 accessions were divided into four major groups by UPGMA clustering method. Group I included L. anhuiensis, L.1ongituba, L. longituba var. flava, L. aurea, L. chinensis, and L. albiflora. Group II included L. incarnata, L. squamigera, L. sprengeri, L. haywardii and L. rosea. Group III consisted of L.radiata, L. radiata var. pumila, L .straminea and L. houdyshelii. The N. tazetta L. var. chinensis was as an independent group IV. The molecular cluster result of SSR marker not only supports the likelihood hybrid origin of L. albiflora, L. squamigera, L. haywardii, L. houdyshelii and L .straminea, but also matched well with the results of chromosomal, karyotypical, and morphological classification. Except of L. albiflora, these species whose basic chromosome number similar or equal to x =11 and karyotypes including 11t or multiples were clustered together respectively, while other species whose basic chromosome number was not x =11 but with 6M+10T karyotypes were clustered together. The same species L. anhuiensis at different localities or L. longituba which has different flower color can be distinguished easily by SSR-PCR technique, and can also the morphological very similar species such as L. radiata and L. radiata var. pumila, L. aurea and L. chinensis. According to the results, we could conclude that L. anhuiensis as an independent species was not supported, it should be recognized as a variety or hybrid of L. longituba due to their very close relationships, and could provide reference for classification, especially germplasm identification, and the cross breeding of Lycoris. References | Related Articles | Metrics

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Histochemical Localization of Carbohydrate and Protein during Flower Bud Differentiation in Oncidium PENG Fang,TIAN Min,WANG Cai-xia,WEI Peng-xiao 2011, 40(04): 8-11.  DOI: 10.3969/j.issn.1009-7791.2011.04.002 Abstract ( )   PDF (520KB) ( )   The flower buds in different development stages of Oncidium ‘milliongolds' were used as tested materials，and the changes of carbohydrate and protein during flower bud differentiation were studied by PAS method and histochemical coloration. The results showed that carbohydrate and protein mainly distributed in or around the apex of primordium. In inflorescence primordium differentiation phase, the content of carbohydrate was much less, while protein was richer and distributed uniformly. With the continued differentiation of the flower bud, carbohydrate and protein occurred abundantly in sepal primordium differentiation phase, and decreased obviously in petal primordium differentiation phase. The carbohydrate and protein focused on gynostemium and pollinia during gynostemium and pollinia differentiation phase. The contents of carbohydrate and protein in flower buds during Oncidium development process were determined, which showed that the change trends of carbohydrate and protein were consistent with the histochemical localization. References | Related Articles | Metrics

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Study on Microsporogenesis and Development of Male Gametophyte in Cardiocrinum giganteum GUAN Wen-ling,LI Shi-feng,LEI Li-ping,LI Ye-fang,CHEN Xian 2011, 40(04): 12-15.  DOI: 10.3969/j.issn.1009-7791.2011.04.003 Abstract ( )   PDF (457KB) ( )   The microsporogenesis and development of male gametophyte in Cardiocrinum giganteum．The results show that the anther has four chambers and the structure of anther wall is the monocotyledonous type，which is composed of epidermis，endothecium，middle layer and glandular tapetum，some cells of the endothecium become fibrously thickened at anther dehiscence．The cytokinesis of microspore mother cell meiosis is successive, and the tetrads are isobilatera1, occasionally tetrahedral．The mature pollen grains are the bicellular type with one germ-pore．The TTC stain test showed the average vitality rate of mature pollen grains was 86.3%. The microsporogenesis and the male gemetophyte development are normal on the whole, producing a great number of normal pollen grains. were observed by the means of conventional paraffin sections and norm slice-making method References | Related Articles | Metrics

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Study on Diurnal Variation of Photosynthetic Indexesof Cymbidium lowianum Cultivated in Different Mediums SUN Hui-jing,WANG Qi,GUO Hong,LI Zhi-lin,WANG You-guo 2011, 40(04): 16-19.  DOI: 10.3969/j.issn.1009-7791.2011.04.004 Abstract ( )   PDF (293KB) ( )   The diurnal variation of photosynthetic indexes of Cymbidium lowianum cultivated in 5 kinds of cultivation medium was determined adopting LI-6400 photosynthetic performance. The results showed that the diurnal variation of photosynthetic indexes such as net photosynthetic rate, transpiration rate, intercellular CO2 concentration, stomatal conductance, were of the same trend, presented a double-peak curve. The change value of net photosynthetic rate, intercellular CO2 concentration and stomatal conductance of Cymbidium lowianum cultivated in medium Ⅳ were relatively large, next came medium Ⅲ, and there were significant differences between mediumⅣ, Ⅲ  and medium Ⅴ (CK) (p<0.05). In general, the medium Ⅳwas the optimum culture medium for the growth of Cymbidium lowianum. References | Related Articles | Metrics

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Studies on the Community Characteristics of Rhododendron fortunei in Jinggang Mountain DENG Xian-lan,LIU Peng,WU Yang,GUO Zhi-hui 2011, 40(04): 20-25.  DOI: 10.3969/j.issn.1009-7791.2011.04.005 Abstract ( )   PDF (316KB) ( )   The species composition, floristic elements, community structure and species diversity of Rhododendron fortunei communities in Jinggang Mountain were analyzed, according to the statistics from 10 sample plots. The result showed that there were 83 species of vascular plants in these communities of Rhododendron fortunei (including varieties), belonging to 44 families and 67 genera, in which the pteridophyte accounted for 6 families, 6 genera and 6 species, the gymnosperm 4 families, 4 genera and 5 species, and the angiosperm 34 families, 57 genera and 72 species. The floristic analysis showed that the flora geographical elements were complex, the tropicail element and temperate element took a large proportion. Communities of Rhododendron fortunei was many storied all-aged forest, Rhododendron fortunei, Daphniphyllum macropodum and Rhododendron simiarum were the constructive species. The Rhododendron fortunei communities in Jinggang Mountain had high Simpson index and Pielou index，low Shannon-Wiener index and Richness index. There were some differences of species diversity among plots, and the variation patterns of species diversity index which was ever used were discordant among layers. References | Related Articles | Metrics

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Study on Plant Diversity and Vegetation of Nansha District, Guangzhou City HE Zhi-fang,CHEN Hong-feng,ZHOU Jing-song 2011, 40(04): 26-31.  DOI: 10.3969/j.issn.1009-7791.2011.04.006 Abstract ( )   PDF (319KB) ( )   Based on field survey, the plant diversity and vegetation of Nansha District, Guangzhou City was studied. There are a total of 603 native vascular plant species belonging to 388 genera, 128 families in Nansha district, and the dominating vegetation are wetland vegetation, aquatic vegetation, beach vegetation and hilly vegetation. The diversity and geographical area of the wild plant in Nansha district were analyzed, and the results indicated that few families are the dominant components of flora, and tropical elements predominate in the flora but there is a degree influence of temperate elements. Finally, suggestions for the protection of plant diversity were proposed. References | Related Articles | Metrics

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Integration of Water into Fertilizer on Banana Cultivation in Xerothermic Area in Hainan Province ZANG Xiao-ping,MA Wei-hong,ZHANG Cheng-lin,ZHOU Zhao-xi,HAN Li-na,ZHONG Shuang,LIU Yong-xia 2011, 40(04): 32-37.  DOI: 10.3969/j.issn.1009-7791.2011.04.007 Abstract ( )   PDF (333KB) ( )   Due to the arid climate, unequal seasonal distribution of water resource and non-scientific fertilizer application, the banana industrial development in xerothermic area in Southwest Hainan province is strongly constricted. Water and nutrient are the two key factors in the growth of banana. The development of water-saving irrigation is the only way to resolve drought in xerothermic area in Southwest Hainan province. Integration of water into fertilizer has lots of special advantages in irrigation-agriculture since it provides the most effective way of supplying nutrients to the plant roots. Integration of water into fertilizer is advantageous to save the cost of water, fertilizer and labor, and there are more economic benefits in integration of water into fertilizer. References | Related Articles | Metrics

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Study on the Selection of Bretschneidera sinensis Elite Sapling with ‘t’ Determination Principle GUO Xiang-quan,LI Yan-qun,SUN Xiao-dong,XIONG Zi-hua,GUO Xiang-dui,YANG Pei-hua,ZHAN Yu-lian,XU Yun-liang 2011, 40(04): 38-42.  DOI: 10.3969/j.issn.1009-7791.2011.04.008 Abstract ( )   PDF (305KB) ( )   The height and rooting-diameter growth characteristics of the 5-year sapling of Bretschneidera sinensis were investigated respectively in the standard sample spot of elite-trees by large sample method, with the primarily selected dominant trees as investigation center. The height and rooting-diameter growth characteristics were both classified and analyzed by mathematical statistics. The result indicated that the growth phenotypic differentiation of height and rooting-diameter in sapling colony of Bretschneidera sinensis followed the normal distribution, and 2-elite were selected with the selection criterion according to the ‘t’ determination principle. The height and rooting-diameter growth of those selected 5-year elite sapling increased by over 115.8%、82.9% and 251.9%、103.7% respectively than the average of location colony. References | Related Articles | Metrics

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nvestigation and Analysis on Yield and Quality of ‘Lingfeng’ Grape on Different Site Conditions HUANG Feng-zhu,PENG Hong-xiang,QIN Xian-quan,XU Ning,ZHU Jian-hua,LU Gui-feng,LI Dong-bo,LI Hong-li 2011, 40(04): 43-45.  DOI: 10.3969/j.issn.1009-7791.2011.04.009 Abstract ( )   PDF (235KB) ( )   The new cultivar of ‘Lingfeng’ grape was used as test material to study the yield and quality on different site conditions in the southeast and central of Guangxi. The results showed that the yield and quality were both higher in fertilizer-rich soil of southeast Guangxi than that of poor soil of central Guangxi, but soil condition only influenced on the growth vigor and yield, and no obviously on quality. We also proposed a cultivation technical measure for high yield and quality of ‘Lingfeng’ grape in southern wet and warm region. References | Related Articles | Metrics

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Research on Fruit Characteristics of Camellia oleifera ‘ChangLin 4’ at Different Canopy Position CAO Yong-qing,WANG Kai-liang,LIN Ping,REN Hua-dong,YAO Xiao-hua,LONG Wei,YIN Run-fu 2011, 40(04): 46-49.  DOI: 10.3969/j.issn.1009-7791.2011.04.010 Abstract ( )   PDF (260KB) ( )   The five years old ‘Changlin 4’ oil-tea camellia was selected to study the effect of different canopy position on fruit characteristics. The results showed that the fruits bearing upper outside the canopy, which average weight was 29.5 g and average number of seeds was 4.8, were both higher than that inside the canopy. While the fresh ratio of seeds and fruit shape index of the fruits bearing upper outside the canopy were lower, which was 46.8% and 0.81 respectively. The oil content ration in seeds of fruits bearing upper outside the canopy was 41.2% (α=0.05) which was significantly higher than that bearing inside the canopy. And no difference of oil content ration in seeds was found among fruits bearing on the different position of outside canopy. In addition, the fat acid composition of seeds was not significantly affected by different bearing position in canopy, and the quality of tea oil was also not influenced by growth zone of fruits greatly. References | Related Articles | Metrics

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Study on the Relationship between the Growth of Eucalyptus urophylla and Its Slope Aspect, Slope Position WEI Hua 2011, 40(04): 50-51.  DOI: 10.3969/j.issn.1009-7791.2011.04.011 Abstract ( )   PDF (205KB) ( )   The relationship between the growth of three-year-old Eucalyptus urophylla in Shouning County, Fujian Province and different slope aspects, slope positions was studied in this article. Through comparative analysis, the result showed that: there was significant difference existing in growth of Eucalyptus urophylla among different slope aspects and different slope positions. Sunny slope could obviously promote Eucalyptus urophylla growth, with its middle and lower part was the most favorable for growth. The difference among different slope positions was also significant. References | Related Articles | Metrics

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Effect of Waste Battery Lixivium on Vicia faba Seed Germination and the Mitosis of Root Tip Cells ZHAO Hong,LIN Guo-wei,LUO Zhao-hui,LIN Tao,HUANG Hong-qin 2011, 40(04): 52-55.  DOI: 10.3969/j.issn.1009-7791.2011.04.012 Abstract ( )   PDF (223KB) ( )   Vicia faba seeds were used as test material for studying the effects of different concentration of waste battery lixivium on their germination and mitosis of root tip cells. The results showed that different concentration of waste battery lixivium could restrain the germination of Vicia faba’s root, bud and the mitosis of root tip cells in a certain degree. The inhibition increased with the increase of concentration, and the micronucleus rate also increased rapidly. The experiment also showed that higher concentration of waste battery lixivium had very strong toxicity, and was heavy pollution solution References | Related Articles | Metrics

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Research on the Micronucleus of Root Tip Cells in Vicia faba Induced by Metronidazole LI Hong 2011, 40(04): 56-58.  DOI: 10.3969/j.issn.1009-7791.2011.04.013 Abstract ( )   PDF (371KB) ( )   The effect that metronidazole induces the micronucleus of root tip cells in Vicia faba was mainly studied in this experimentation. By this study, it showed that: (1) When the metronidazole concentration was 0.1 mg/L, 6 mg/L, 12 mg/L, 40mg/L and 500 mg/L, the micronucleus frequencies of root tip cells in Vicia faba was significantly different from that of negative control group, and there was apparent dosage—effect relationship between the concentration of metronidazole and the micronucleus frequencies of root tip cells in Vicia faba；(2) Metronidazole could cause the damage of genetical materials (DNA), and it had the characteristic of molecular mutagenesis. References | Related Articles | Metrics

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Residual Dynamics of Isocarbophos in Banana and Soil YANG Shu-juan,GUO Jian-hui,CAI En-xing,CHEN Li-ping 2011, 40(04): 59-62.  DOI: 10.3969/j.issn.1009-7791.2011.04.014 Abstract ( )   PDF (220KB) ( )   By using the methods of GC and the field tests, the residual degradation dynamics of isocarbophos was studied in bananas and soils. The results showed that the initial sediment and residue of isocarbophos were closely related to the application dosage, and comparing to the recommended dosage, the initial sediment of the doubling dosage rose by 48%.The residual degradation dynamics of isocarbophos in banana and soil accorded with the first-order kinetics, with the correlation coefficients (︱r︱) located between 0.9465 to 0.9490 (P＜0.01), the degradation coefficient(︱k︱) was 0.1606±0.0035, T1/2 ranged from 4.3 d to 4.5 d, and T0.99 ranged from 28.1 d to 29.4 d in banana; while in soil the︱r︱, the︱k︱, T1/2 and T0.99 parameters were 0.9552(P＜0.01), 0.2107, 3.3 d, 21.9 d respectively. The isocarbophos residues of final product banana and soil couldn’t still be detected after the last application 58~65 days. References | Related Articles | Metrics

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Primary Identification on Fruit Pigment of Elaeagnus conferta Roxb. LIU Yu-mei,HUANG Wei-nan 2011, 40(04): 63-65.  DOI: 10.3969/j.issn.1009-7791.2011.04.015 Abstract ( )   PDF (341KB) ( )   The fruit pigment of Elaeagnus conferta Roxb. was identified to be fat-soluble carotenoid by physicochemical property and spectrum analysis. The carotenoid was separated into seven pigment zones by TLC on fiber and silica gel with one eluant of petroleum ether/ acetone/ benzene (2:0.5:2, V/V). The maximum carotenoid was identified as γ-carotene by UV-visible and IR spectrum analysis. References | Related Articles | Metrics

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A Preliminary Study on the Damage Characteristics of Liothrips sp. in Landscape Plants and Its Control Measure ZHAN Fu-lin,LUO Jia,JIANG Fan 2011, 40(04): 66-68.  DOI: 10.3969/j.issn.1009-7791.2011.04.016 Abstract ( )   PDF (264KB) ( )   This article reported a new pest, which resulted in the death of the branches, frizz of the twigs, and lots of galls on the leaves of ornamental plants called Fagraea ceilanica in Xiamen and its surrounding area. It was identified as Liothrips sp. In addition, the damage characteristics, morphological identification and relationship with environment of Liothrips sp. were described in detail, and the control strategies and other relevant issues were also discussed. References | Related Articles | Metrics

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Two New Recorded Genera in Guangzhou and their Geographic Significance TANG Guang-da,ZHUANG Xue-ying,QIU Jian-li,MA Hong-yan,ZHENG Ming-xuan,XU Zheng-chun,HOU Bi-qing 2011, 40(04): 69-70.  DOI: 10.3969/j.issn.1009-7791.2011.04.017 Abstract ( )   PDF (184KB) ( )   Two new recorded plants, S. nepalensis Wall. and P. bulbocodioides (Franch.) Rolfe, respectively belonging to Sarcopyramis of Melastomatceae and Pleione of Orchidaceae, were reported in Guangzhou City, Guangdong Province. The voucher specimens were deposited in the Herbarium of Forestry College, South China Agricultural University (CANT). The two species were found at high 260～300 m altitude in Conghua, which was the southernmost and the lowest altitude records for them so far. The new records would provide important references for the effects of diffusion mechanism, vertical distribution, climate change on species migration, and some other theoretical problems. References | Related Articles | Metrics

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Study on Cutting Propagation Technique for Baphicacanthus cusia ZENG Qing-qian,YANG Hong-mei,HUANG Shan-shan,MO Xiao-lu 2011, 40(04): 71-74.  DOI: 10.3969/j.issn.1009-7791.2011.04.018 Abstract ( )   PDF (237KB) ( )   The effects of different matrix composition, different position, different pitch number and different plant growth regulator on cutting propagation of Baphicacanthus cusia were investigated. The results showed that all of those 4 factors had remarkable effects on rooting occurrence. The best composition of matrix was 2 part river sand plus 1 part perlite, which resulted in 93.5% rooting rate; cuttings from top fragment made high rooting rate, up to 94.2%; the rooting rate of 2 pitch cuttings was 90.7%; and 100mg/L IBA lead to the highest rooting rate, up to 94.5%. Appropriate methods were beneficial to increase the propagation coefficient of Baphicacanthus cusia. References | Related Articles | Metrics

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Study on Initiation Culture of Leucadendron ‘Kelvin Red’ HE Li-na,MA Lin,JIANG Yan,CHEN Jing,PAN Hui-tang, 2011, 40(04): 75-78.  DOI: 10.3969/j.issn.1009-7791.2011.04.019 Abstract ( )   PDF (247KB) ( )   The initiation culture of in vitro rapid propagation in Leucadendron ‘Kelvin Red’ was investigated. The results showed that the best method for explants disinfection was as follows: the explants were surface-disinfested in a solution of 75% alcohol for 30 seconds and then dipped into 0.1% mercuric chloride two times for 5 minutes and 4 minutes respectively. Winter was the optimal season for collecting explants. It was found that shoot tips and the upper semi-lignified stems were the optimal explants materials. The optimal startup medium for in vitro Leucadendron propagation was WPM + 6-BA 1.0 mg/L + IBA 0.5 mg/L. References | Related Articles | Metrics

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Tissue Culture of Anoectochilus moulmeinensis LI Hong-lin,YANG Bo 2011, 40(04): 79-79.  DOI: 10.3969/j.issn.1009-7791.2011.04.020 Abstract ( )   PDF (155KB) ( )   Related Articles | Metrics

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Research Progress of Ammonia Volatilization in Turf Ecosystem WANG Qi,SHI Chun-juan 2011, 40(04): 80-84.  DOI: 10.3969/j.issn.1009-7791.2011.04.021 Abstract ( )   PDF (207KB) ( )   The research results of ammonia volatilization in turf ecosystem were summarized, especially for the mechanism of ammonia volatilization, influencing factors, determination methods, and the approach of reducing volatilization, which aimed to provide theoretical guidance for reasonable and effectively fertilizer application. References | Related Articles | Metrics

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The Research Situation and Prospect of Woody Vegetables ZHANG Feng-yun,ZHONG Feng-lin,HUANG Hui-yan,YANG Bi-yun,HAN Zhi-jun,LI Liang,LIN Yi-zhang 2011, 40(04): 85-88.  DOI: 10.3969/j.issn.1009-7791.2011.04.022 Abstract ( )   PDF (254KB) ( )   Woody vegetable is one natural, green and edible wild vegetable. This paper briefly introduces the current problems in the development and utilization of woody vegetable and analyzes the reasons that woody vegetable can be planted as pollution-free vegetable. In addition, it also points out that pollution-free cultivation is the best way to keep the vegetable’s wild, nature, and pollution-free characters and makes prospect for pollution-free cultivation. References | Related Articles | Metrics

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Research Advances of Conservation Biology on Dysosma versipellis ZHANG Yan,LI Bin,LI Si-feng 2011, 40(04): 89-92.  DOI: 10.3969/j.issn.1009-7791.2011.04.023 Abstract ( )   PDF (233KB) ( )   Dysosma versipellis is a rare and endangered plant which is endemic to our country. According to the field work and literature review, the morphological characteristics, geographical distribution, ecological environment, endangerment situation as well as current protection and research status were reviewed in this paper. On the basis of emphatically analysis on the protection situation of Dysosma versipellis, some suggestions were proposed, which included doing protection word well, enhancing study on genetic diversity, reproductive ecology and developmental biology of Dysosma versipellis. References | Related Articles | Metrics