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Molecular Identification of Cymbidium mosaic Virus Infecting Orchid in Xiamen GUO Ying,LIN Zhi-kai,LIU Li-qing,LIN Qing-hong 2011, 40(03): 1-3.  DOI: 10.3969/j.issn.1009-7791.2011.03.001 Abstract ( )   PDF (278KB) ( )   Based on the reported Cymbidium mosaic virus (CyMV) genomic cDNA sequence, specific primer of CymV-CP was designed, and RT-PCR was performed with the total RNA as templates extracted from diseased orchid plant collected from Xiamen, to attain complete CP gene sequence. The result was that the virus isolates of XM orchid had high homology with isolates that separated form other areas of China and Asian countries (the homologies were all above 96% at nuclear acid). According to phylogenetic tree analysis, the virus isolates of XM orchid clustered with reference sequences of Taiwan, Korea and India, and Fujian, Beijing and Singapore isolates clustered together. References | Related Articles | Metrics

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Preliminary Study on Sterility of Plumeria rubra MA Huan,TIAN Hui-qiao 2011, 40(03): 4-7.  DOI: 10.3969/j.issn.1009-7791.2011.03.002 Abstract ( )   PDF (299KB) ( )   The plant of Plumeria rubra blooms but has no seeds. The study on this species was conducted to discover the cause of its sterility. The development of embryo sac and pollen were both normal using section observation on female and male organ and pollen germination in vitro, and pollen in vitro could germinate pollen tubes. The cylinder of corolla is about 1cm length, and its basal has high humidity. When blooming, the pistil stigma and anthers can not elongate out the cylinder. Therefore, the anthers staying inside humid cylinder can not dehisce and release pollen, which makes obstacle for pollination, and finally causes sterility. References | Related Articles | Metrics

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Studies on Grafting Affinity of New Varieties of Litchee ‘Guifeihong’ and ‘Caomeili’ HUANG Feng-zhu,PENG Hong-xiang,ZHU Jian-hua,LI Hong-li,LI Dong-bo,XU Ning,QIN Xian-quan,DING Feng,LU Gui-feng 2011, 40(03): 8-11.  DOI: 10.3969/j.issn.1009-7791.2011.03.003 Abstract ( )   PDF (215KB) ( )   Using ‘guifeihong’ Litchee, ‘Caomeili’ Litchee and ten different rootstocks as the test material, determination and comparison of grafting survival rate and field growth index in various stock-scion combinations were conducted. The results were as follows: Grafting survival rate and field growth potential was obvious difference in various stock-scion combinations. For ‘Guifeihong’ Litchee, the grafting survival rate of ‘Qinzhou red’ Litchee, ‘Heiye’ and ‘Heli’ was respectively 80.0%, 78.0% and 74.0%; For ‘Caomeili’, grafted on ‘Heli’ ,the survival rate was the highest up to 82.0% with strong growth potential and smooth graft union ; The grafting survival rate and field growth potential of grafted plants was corresponding. References | Related Articles | Metrics

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Effects of Spraying Different Growth Regulators on Oncidium Florescence PENG Fang,WEI Peng-xiao,TIAN Min,CEN Xiu-fen 2011, 40(03): 12-15.  DOI: 10.3969/j.issn.1009-7791.2011.03.004 Abstract ( )   PDF (205KB) ( )   Using the division seedlings of Oncidium variety named ‘milliongolds’ as test materials，the effects of spraying treatments with different kinds and concentrations of plant growth regulators on Oncidium florescence were studied. The results showed that the blossom date accelerated significantly by spraying 200 mg/L GA3 and GA3 (200 mg/L) + 6-BA (25 mg/L), compared with the control treatment (no spraying growth regulators). Single treatment with 6-BA could improve the rate of flower bud differentiation but make the flower getting smaller. Spraying GA3+6-BA could not only enlarge the flowers size, but also prolong stalk length. The blossom time prolonged significantly by the treatments of spraying 200 mg/L and 250 mg/L PP333, especially 250 mg/L treatment. Besides, the stalk length also decreased by PP333 treatments. There was no remarkably effect on the first internode width of pedicle in all growth regulator treatments. References | Related Articles | Metrics

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Variation of Soluble Sugar, Protein and POD Activity in Bulbs of Two Lycoris during Growth and Development HUANG Xue-fang,JIN Ya-qin,LI Dong-lin 2011, 40(03): 16-19.  DOI: 10.3969/j.issn.1009-7791.2011.03.005 Abstract ( )   PDF (223KB) ( )   Variation of soluble sugar, protein and POD activity in bulbs of both Lycoris chinensis and Lycoris radiata during the growth and development were studied. The results showed both soluble sugar and protein of two Lycoris plants increased and appeared peak value during the prophase of flower bud differentiation, but the content of soluble sugar decreased with the coming of florescence. During the florescence, the soluble protein content of two Lycoris lied in high level, and decreased after florescence. During the leaves growth the soluble protein content of Lycoris radiata rose evidently, and that of Lycoris chinensis reversed. During the prophase of flower bud differentiation, the POD activity in bulbs of two Lycoris ascended, while the anaphase slightly descended. After florescence, the POD activity in bulbs of two Lycoris appeared brief low value and soon after moved up with the temperature reduction. References | Related Articles | Metrics

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Effects of Intermittent Warming and Heat Treatment on Postharvest Physiology of Plum Fruits SHI Hui,LONG Chao-an,YE Zu-yun 2011, 40(03): 20-24.  DOI: 10.3969/j.issn.1009-7791.2011.03.006 Abstract ( )   PDF (266KB) ( )   Plum (cv. Purple) fruits were treated with intermittent warming and heat treatment after harvest, with their brown degree, antioxidant enzyme activities，antioxidant content, membrane peroxidation level，total phenols and titratable acid contents were investigated. The results showed that intermittent warming and heat treatment could properly restore antioxidant activity depressed by chilling injury, eliminate active oxygen free radical, reduce the accumulation of membrane peroxidation production MDA, inhibit the activities of PPO and POD, strengthen resistant system to low temperature and prevent polyphenol metabolism by adversity, thus deferred and lightened chilling injury and browning symptom. At the same time, both treatments also inhibited titratable acid content decreasing and SSC/TA increasing, postponed mature and senescence. Plum fruits could be stored in intermittent warming condition for 60 days without other treatments. We preliminarily considered that this change-temperature treatment pattern which temperature raised to 18～20℃ for 24 hours every 15 days during cold storage at －0.5～0℃ was the optimal storage condition for ‘Purple’ plum. References | Related Articles | Metrics

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Effect of Different Phosphorus Level on Output and Quality of Banana HUANG Da-bin?,LIN Xiang-hua?,LIN Hui-huan?,HUANG L?-qing?,CHEN Qiao-ming? 2011, 40(03): 25-28.  DOI: 10.3969/j.issn.1009-7791.2011.03.007 Abstract ( )   PDF (221KB) ( )   The effect of different phosphorus level on output and quality of banana under the conditions of quantitative N & K2O application was studied, using the field test method with single factor analysis. The results showed that banana trees, which were planted in plantation with appropriate available phosphorus soil, fertilized by 0.87kg urea, 1.335kg KCl, 2.0kg organic fertilizer, 125.0g magnesium sulfate per plant, sprayed by 0.2% borax solution one time, especially fertilized by 1.165kg calcium superphosphate per plant and N∶P2O5∶K2O = 1.0∶0.35∶2.0, grew fine. There were 3 advantages of this prescription: firstly, banana trees grew faster with their pseudostem much thicker, and number of functional leaves at the harvest were more, harvest time was earlier and shorter, output was higher; simultaneously, the quality and appearance of fruit was much better, for banana became longer and thicker, starch and total sugar content was much higher, and the edibility rate was also a little higher, titratable acid was medium; in addition, the economic efficiency reached to the best, with the net profit attained to 2637.6 RMB /667m2 , and the input-output ratio 1∶1.83. References | Related Articles | Metrics

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Effects of NaCl Stress on Bauhinia Pollen Germination XU Fen-fen,ZHOU A-feng,LIU Xia,YUAN Pan 2011, 40(03): 29-30.  DOI: 10.3969/j.issn.1009-7791.2011.03.008 Abstract ( )   PDF (164KB) ( )   The effects of NaCl with different concentrations on pollen germination characteristics of Bauhinia (Cercis chinensis) were studied by artificial culture method on pollen with Bauhinia as test materials. The results showed that Bauhinia pollen germination was inhibited by NaCl, and the inhibition enhanced with the increasing of NaCl concentrations. Bauhinia pollen germination was significantly (P<0.05) inhibited under 20mmol/L NaCl, and pollen tube growth was significantly (P<0.05) inhibited under 5mmol/L NaCl. References | Related Articles | Metrics

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Preliminary Study on Screening Anti-Tumor Active Fraction of Extracts from Seven Chinese Herbal Medicines ZHENG Zhi-zhong,CHEN Liang-hua,ZHENG Guo-hua,TONG Yu-xing,ZhANG Shu-feng,TONG Qing-xuan,MING Yan-lin 2011, 40(03): 31-35.  DOI: 10.3969/j.issn.1009-7791.2011.03.009 Abstract ( )   PDF (262KB) ( )   Though extracting and separating on seven Chinese herbal medicines including Phyllanthus urinaria Linn., Phyllanthus niruri Linn., Senecio scandens Buch. -Ham. ex D. Don, Brucea javanica (Linn.) Merr, Quisqualis indica Linn., Ilex chinensis Sims and Macfadyena unguis-cati(Linn.)A.Gentry, the anti-tumor active fractions were screened, and their anti-tumor effects on different kinds of human tumor cell lines were preliminary analyzed. The proliferation inhibitory effects in vitro of alcohol extracts on liver cancer, gastric cancer, oophoroma and other human tumor cell lines were studied by MTT assay to screen active fractions, with the normal hepatocyte lines as contrast. The experimental results showed that alcohol extracts, from all of seven Chinese herbal medicines, contained chemical separation fractions, which had proliferation inhibitory effects on tumor cell lines. Among those fractions, ethyl acetate layer and n-butanol portion of Phyllanthus urinaria and Phyllanthus niruri had remarkable inhibitory effects on different tumor cell lines respectively. Ethyl acetate layer of Senecio scandens, n-butanol portionof Brucea javanica, chloroform layer of Quisqualis indica and Ilex chinensis took second place. Chloroform layer of Macfadyena unguis-cati andethyl acetate layer of Ilex chinensis were of weaker than the former relatively. Others had not activities. References | Related Articles | Metrics

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In Vitro Germination and Plantlet Regeneration from Seeds of Staphylea holocarpa YU Dao-ping,LI Ce-hong,XIE Kong-ping,ZHANG Guo-zhen,GU Hai-yan,LI Shi-li, 2011, 40(03): 36-40.  DOI: 10.3969/j.issn.1009-7791.2011.03.010 Abstract ( )   PDF (813KB) ( )   null References | Related Articles | Metrics

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Studies on Explants Selection and Callus Induction of Anoectochilus roxburghii WANG Ya-ying,LIN Xiao-hua,HONG Xuan 2011, 40(03): 41-43.  DOI: 10.3969/j.issn.1009-7791.2011.03.011 Abstract ( )   PDF (229KB) ( )   With the stems, leaves, segments, buds of Anoectochilus roxburghiias as explants, the induction of callus was studied by culturing at 5 different culture mediums with 6-BA, ZT, NAA, KT respectively. The results showed that explants including stems, segments and buds could successfully induce callus occurrence at following culture mediums, MS + 6-BA 2.0 mg/L + NAA 0.5 mg/L, MS + NAA 2.0 mg/L + KT 0.1 mg/L and MS + 6-BA 2.0 mg/L + NAA 0.5 mg/L + ZT 0.2 mg/L. Certainly, 6-BA, NAA, KT were the important factors on production of callus. Among the three explants, the buds were the best explants for the callus induction. And the results of orthogonal design showed that for the bud of Anoectochilus roxburghii to form the callus, the best medium was MS + 6-BA 2.0 mg/L + NAA 0.5 mg/L + KT 0.2 mg/L. References | Related Articles | Metrics

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The Effect of 6-BA and 2,4-D on Proliferation, Differentiation and Conservation in vitro of Dendrobium candidum HONG Sen-rong,FANG Mei-ling 2011, 40(03): 44-46.  DOI: 10.3969/j.issn.1009-7791.2011.03.012 Abstract ( )   PDF (269KB) ( )   The influence of 6-BA and 2,4-D on proliferation, differentiation and conservation in vitro of Dendrobium candidum was discussed in this paper. The results showed that the better medium for D. candidum protocorm shoot regeneration was MS. The better medium for D. candidum protocorm regenerated bud growth was MS + 6-BA 1mg/L + 2,4-D 0.1 mg/L. The better medium for D. candidum protocorm regenerated bud rooting and protocorm proliferation were also MS. Adding 0.5 mg/L 2,4-D was bad for conservation of D. candidum protocorm, but without 2,4-D or adding 0.1 mg/L 2,4-D contributed to conservation of D. candidum protocorm which had sustained growth trend. References | Related Articles | Metrics

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Study on Browning Control in Callus Tissue Culture of Cunninghamia lanceolata CHEN Jian-yong 2011, 40(03): 47-49.  DOI: 10.3969/j.issn.1009-7791.2011.03.013 Abstract ( )   PDF (207KB) ( )   Abstract: Influences of 4 kinds of antioxidants (Vitamin C, Citric acid, Cysteine, Sodium thiosulfate) and active carbon on callus browning of Cunninghamia lanceolata were studied with the callus induced from its stem tip as experimental materials. The result indicated that, Vit C and Cys could efficaciously control callus browning and had no inhibitory action on callus growth. So they were anti browning agents of more suitable for tissue culture of Cunninghamia lanceolata. References | Related Articles | Metrics

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Study on Rapid Propagation of Stem Segments of Dendrobium devonianum LI Shou-ling,BAI Yan-bing,GAO Yan,LI Ze-sheng,GENG Xiu-ying,WANG Ying-qing,WU Rui 2011, 40(03): 50-52.  DOI: 10.3969/j.issn.1009-7791.2011.03.014 Abstract ( )   PDF (203KB) ( )   The rapid propagation used in stem segments of Dendrobium devonianum was studied in this paper. The results showed that the best suitable medium for Dendrobium devonianum induction was MS + 6-BA 2.0 mg/L + NAA 0.05 mg/L, and MS + 6-BA 3.0 mg/L + NAA 0.5 mg/L, MS + IBA 1.0 mg/L + NAA 0.5 mg/L were the mediums respectively for its bud proliferation and rooting. References | Related Articles | Metrics

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Application of Ultrafiltration Technology on the Extraction of Seaweed Polysaccharide YU Li-guo,WU Yong-pei,ZHOU Yi 2011, 40(03): 53-56.  DOI: 10.3969/j.issn.1009-7791.2011.03.015 Abstract ( )   PDF (242KB) ( )   In this paper, 50 KD of ultrafiltration membrane was used to purify the extraction of Porphyra haitanensis polysaccharide. Results showed that the pigments could be effectively removed from extraction mixture by ultrafiltration and the rate of decolorization was 91.65%. Furthermore, during the process, the physiological activator of polysaccharide wasn’t damaged, simultaneously purified the sample, for the total sugar of the sample increased from 34.95% to 52.07%. References | Related Articles | Metrics

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Determination of Buprofezin, Phosmet and Pyridaben in Tea Sample by Ion Trap Gas Chromatography-Mass Spectrometry SHANGGUAN Liang-min 2011, 40(03): 57-59.  DOI: 10.3969/j.issn.1009-7791.2011.03.016 Abstract ( )   PDF (229KB) ( )   A qualitative and quantitative analysis method was developed for determination of buprofezin, phosmet and pyridaben by gas chromatography – ion trap tandem mass spectrometry in tea samples which were extracted and enriched by solid phase extraction. The samples were extracted by acetonitrile, purified by activated carbon method, concentrated and constant volume to analyze. In this experiment, HP–5MS capillary column was used to separate, inspective mode electron bombardment ion source MS was adopted to detect, and external reference method was employed to quantify. The result indicated that the linear relationship of the method was favorable with R ≥ 0.9970, and the minimum detectability was 0.01~0.02 mg/kg, with the fortified recovery of 3 pesticides ranged from 92.0% to 105.0%, RSD 3.0%～9.3%. The method was simple, high efficiency and sensitivity for the simultaneous determination of buprofezin, phosmet and pyridaben in tea samples. References | Related Articles | Metrics

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Growth Adaptability of Cunninghamia lanceolata of Advanced Generation Provenance in Different Site Conditions LIAO Shi-shui 2011, 40(03): 60-63.  DOI: 10.3969/j.issn.1009-7791.2011.03.017 Abstract ( )   PDF (218KB) ( )   The growth status of Cunninghamia lanceolata of advanced generation provenance in their 2-years stage was investigated by planting test in different site conditions. The results indicated that their average growth of ground diameter and tree height in their 2-years stage were respectively 4.08, 3.82, 3.56cm and 1.90, 1.74, 1.63m in down-slope, middle-slope and up-slope. According to data analysis, there were also significant differences existing in the growth of ground diameter and tree height, in different site conditions. References | Related Articles | Metrics

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Decline Reasons of Ancient Longan Trees in Quanzhou, Fujian Province and their Rejuvenation Experiments CHEN Feng-hua,HUANG Bi-li 2011, 40(03): 64-67.  DOI: 10.3969/j.issn.1009-7791.2011.03.018 Abstract ( )   PDF (259KB) ( )   This paper investigated the growth status of ancient longan trees in Shiyashan Park in Quanzhou, Fujian province, and also researched their decline reasons. Moreover, we drew up comprehensive measures to carry out salvage rejuvenation, and analyzed the effects of rejuvenation measures on tree vigor and biochemical indicators, which could recover the tree vigor well. References | Related Articles | Metrics

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Bamboo Resource and Its Exploitation and Utilization in Jiufu Mountain Nature Reserve, Fujian Province CHEN Jian-jing 2011, 40(03): 68-71.  DOI: 10.3969/j.issn.1009-7791.2011.03.019 Abstract ( )   PDF (241KB) ( )   There are totally 9 genera, 23 varieties of bamboo resources in Jiufu Mountain Nature Reserve, Youxi County, Fujian Province, mainly including Phyllostachys heterocycla cv. pubescens forests, Acidosasa notata forests, Indocatamus tesselatus forests and Pleioblastus amarus forests. The species, components of taxonomical group, types of distribution region of these bamboo resources were reported in this paper, and some suggestions on how to exploit and utilize these resources in this area were also put forward. References | Related Articles | Metrics

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Screening and Utilization of Wild Ornamental Plant Resources in Liancheng County, Fujian Province LIN Kai-he 2011, 40(03): 72-77.  DOI: 10.3969/j.issn.1009-7791.2011.03.020 Abstract ( )   PDF (309KB) ( )   According to the survey, there are 61 kinds of wild ornamental plants in Liancheng County, Fujian Province, including 44 kinds of arbors and shrubs and 17 kinds of ground lianas. Based on the ornamental value of wild ornamental plants, 16 dominant species of arbors and shrubs and 6 dominant species of ground lianas, which are all of high ornamental value, were selected. Then, the properties and characteristics, and the flowers and fruits characteristics, of the 22 dominant species were described, and their application value and configuration form were also analyzed. In addition, for the requirement of establishing eco-tourism vacation city, some countermeasures about developing wild ornamental flowers and promoting sustainable development of landscape ecological construction were proposed. References | Related Articles | Metrics

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The Terminology for Recording Characteristics of Bougainvillea Fancy Breed ZHOU Qun,HUANG Ke-fu,GUO Hui-zhu 2011, 40(03): 78-80.  DOI: 10.3969/j.issn.1009-7791.2011.03.021 Abstract ( )   PDF (199KB) ( )   The article studied the morphological characteristics of Bougainvillea Fancy Breed, put forward the recorded methods and carried on the terminology to the variety name, trade name, variety origin, twig, inflorescence, flower color, stamen, florescence, fruit morphology, maturation phase and unique evaluation. References | Related Articles | Metrics

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The Ecological Significance of Botanical Volatile Organic Compounds HUANG Ai-kui,LI Nan 2011, 40(03): 81-86.  DOI: 10.3969/j.issn.1009-7791.2011.03.022 Abstract ( )   PDF (303KB) ( )     The gaseous volatile organic compounds (VOCs) from plants change the chemical composition of atmosphere through a series of redox reaction, and play an important role in the formation of ozone, CO and CH4. The oxidation material has a significant influence on local and even global environment and climate. The impacts of Botanical VOCs (BVOCs) on atmospheric chemistry, greenhouse effect and photochemical smog are introduced briefly in this paper. On the base of domestic and foreign research, the releasing mechanism, synthesis route, as well as estimation of emission rate are also recommended. At last, some advices about future studies, extensive planting forest and urban greening are advanced. References | Related Articles | Metrics

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QTL Mapping and Cloning in Plant Genetic Breeding of Disease Resistance LI Hong 2011, 40(03): 87-92.  DOI: 10.3969/j.issn.1009-7791.2011.03.023 Abstract ( )   PDF (359KB) ( )   This paper has summarized the QTL mapping and cloning in plant genetic breeding of disease resistance. It mainly expounded the genetic basis of quantitative resistance, QTL mapping and location of disease resistance in crop, the reliability of QTL mapping and adopted measures and the identification and positional cloning of candidate genes of QTLs, etc., and also discussed the future and perspective of plant genetic breeding of disease resistance. References | Related Articles | Metrics