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10 March 2006, Volume 35 Issue 01 Previous Issue    Next Issue

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Effects of Colletotrichum gloeosporioides Infection on Physiological and Biochemical Changes of Litchi Fruit LI Xin-yun, CHEN Wei-xin, LIU Ai-yuan 2006, 35(01): 1-4.  Abstract ( )   PDF (225KB) ( )   Changes in respiration rate, ethylene production, oxidation and peroxidation process and activities of several enzymes related to phenolic oxidation in litchi fruit were determined after inoculating litchi fruit with Colletotrichum gloeosporioides.The results showed that after inoculation, both respiration rate and ethylene production of litchi fruit enhanced significantly, the rate of superoxide radical anion (O2·) production and lipid peroxidation reactant-malondialdehyde (MDA) increased, the activity of superoxide dismutase (SOD) decreased while polyphenoloxidase (PPO), peroxidase (POD) and phenylalanine ammonia-lyase (PAL) promoted on litchi pericarp.All above indicated that the infection of Colletotrichum gloeosporioides could enhance respiration rate and ethylene production, accelerate the course of oxidation and peroxidation, and induce the activities of POD, PPO and PAL on litchi pericarp.So the infection of Colletotrichum gloeosporioides was one of the important reasons that quickened senescence, browning and decay of litchi fruit. Related Articles | Metrics

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Genetic Diversity Analysis on Eight Turfgrass of Poaceae by RAPD CHEN Yan, HU Mu-lin, YUAN Chang-chun, GUAN Guang-hui, LUO Mei, HUANG Yong-lian 2006, 35(01): 5-8,19.  Abstract ( )   PDF (236KB) ( )   The genetic diversity of eight turfgrass varieties representing four species in four genera of family Poaceae was analyzed using random amplified polymorphic DNA (RAPD) method.Fifteen 10-meroligonucletide primers were used for DNA ampification, and 144 DNA bands were generated, of which 63.2% is polymorphic loci.The genetic distances are 0.0857~0.2928 among eight turfgrass varieties, the maximum value is between the variety Crossfire Ⅱ of Festuca arundinacea and Accent of Lolium perenne, the minimum value is between the two varieties of Unhulled Cynodon dactylon and hulled Cynodon dactylon.The two dendrograms based on genetic distance by UPGMA and Neighbor-joining method are similar topologically.The relationships among different varieties from the same species are very close.The relationships of inter-genera or interspecies are relatively far, although the different genera of the tribe Chlorideae get together into a clade.The four warm-season turfgrass form a clade.The variety Accent of Lolium perenne, a cool-season turfgrass, has a far relationship to other turfgrass and forms a clade.The study didn't support to put the genus Lolium and Festuca into the same tribe Poeae and suggested that the turfgrass Festuca arundinacea be treated as an intergrade type of turfgrass. Related Articles | Metrics

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Influence of Medium and Light Intensity on the Leaf Explant Browning in Phalaenopsis sp.in vitro XU Chuan-jun, LI Ling 2006, 35(01): 9-12.  Abstract ( )   PDF (216KB) ( )   The browning ratio of Phalaenopsis sp.leaf explant in vitro growing on MS liquid paper bridge and 1/2 MS after 10 d is 0 and 67%, respectively, less than that growing on MS, B5 and N6.Doubling the content of FeSO4 or microelement deficiency could increase the browning rate of explant cultured in MS medium.Reducing the content of agar could increase the degree of browning.The higher the intensity of light, the more serious the explant browning.Explant cultured on medium supplemented with citric acid showed a considerable reduction in browning.Adding Vit C, active carbon or PVP could not overcome the browning of explant. Related Articles | Metrics

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Preliminary Study on Fruit Quality of Newhall Orange from Various Spot with Different Latitude BAO Jiang-feng, XIA Ren-xue, DENG Xiu-xin, PENG Shu-ang, LIU Yong-zhong, MA Xiang-tao, ZHANG Hong-yan 2006, 35(01): 13-16.  Abstract ( )   PDF (243KB) ( )   The major quality factors of Newhall orange from various spots with different latitude within orange superior regions were tested.The result of liner regression analysis showed that the content of TSS and TA had a significant, the positive linear relationship with latitude.The ratio of TSS to TA, the peel yellowness, the color strength and the peel color angle had a significant and negative linear relationship with latitude.The Vit C content was positively correlated with latitude. Related Articles | Metrics

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Effect of Different Fertilizers on the Balance and Cumulation of N, P, K Nutrients in Soil OU YANG Tong-jiao, LIN Yong, GUO Wen-jie 2006, 35(01): 17-19.  Abstract ( )   PDF (155KB) ( )   This title discussed the effects of different fertilizers, fertilization configuration and fertilization methods on soil fertility, and also took some researches on the balance and cumulation of N, P, K nutrients in soil.The results showed that the effects of unit N on the surplus of N in soil were dungs of pig> fertilizers> straws, the effects on increasing the whole N in soil were straws> fertilizers> dungs of pig;the effects of unit P on the surplus of P in soil were dungs of pig, the effects on increasing the whole P in soil were fertilizers> dungs of pig> straws;the effects of unit K on the surplus of K in soil were fertilizers> dungs of pig> straws, the effects on reducing the K loss in soil were dungs of pig> straws> fertilizers. Related Articles | Metrics

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Degradation of Dichlorvos Residing in 3 Bean Vegetables and Its Soil GUO Jian-hui, CHEN Li-ping, SHI Tong-hua 2006, 35(01): 20-21,24.  Abstract ( )   PDF (166KB) ( )   Some field experiments were taken on the degrading process of dichlorvos residing in bean vegetables (green soy bean, pea, cowpea) and soil.The results showed that when the 80% dichlorvos oil was used at rates of 500~2 000 times, the degradation rate of dichlorvos was higher than 93%.3 d after sprayed, the degradation rate was over 99%, and the residue content was tested less than 0.02 mg/kg.5 d after sprayed, its residue could not be tested out in any bean vegetable.The degradation of dichlorvos residing in soil for pea growing conform to the regulation of the classical power rate.DT50 of dichlorvos was 4.3 d. Related Articles | Metrics

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Analysis on Karyotype of Saururus chinensis JIANG Nian-qiong, Hayashi H N 2006, 35(01): 22-24.  Abstract ( )   PDF (226KB) ( )   Data of chromosome numbers are very important for a better understanding of the origin and evolution of a given flora.In this paper, Karyological studies were carried out in Saururus chinensis.The result showed that the original number of chromosome of Saururus chinensis is X=11, 2n=22, same as the Saururaceae that most primitive.Saururus chinensis's karyotype formula is 2n=2x=22=2m+8sm+1s, and its karyotype type is 3B. Related Articles | Metrics

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Studies on Female and Male Gametophytes Development of Malvaviscus arboreus var.penduliflorus ZHANG Ning, SU Ming-hua, LIN Qing-hong, LIN Jie 2006, 35(01): 25-28.  Abstract ( )   PDF (1644KB) ( )   The anther of Malvavisus arboreus var.penduliflorus consists of epidermis (one layer), endothecium (one layer), middle layer (two layer), tapetum (one layer) and aporgenous cells.The anther has four chambers and the development of anther wall is dicotyledonous type.The microsporogenesis and male gametophyte development are observed as follows:with the meiosis of microspore mother cells, tetrad is formed, of whish the cytokinesis is simultaneous type and the arrangements are tetrahedral, crossing and left-right symmetrical;the microspores complete the mitosis and generate double-nucleus pollen;the generative nucleous complete the mitosis again and generate three-nucleus pollen.The eoidermis disappears at the uninuclear microspore stage, the middle layer disappear at the pollen mother cell stage, the endothecium forms fibrous layer at uninuclear microspore stage, and the tapetum cells disappear at the uninuclear microspore stage and the tapetum is amoeboid one.The female gametophyte development is observed to indicate epigynous ovary, five locules, one ovule per locule, carpylotropous, axial placenta;most of ovules development stop at the nucellus formation stage and a few of nucellus form many archesporied cells, uninuclear and double-nucleus embryos. Related Articles | Metrics

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Study on Enrichment Culture of Asparagus officinalis Cespitose Buds LIN Zong-Keng, LUO Jin-shui, CAI Kun-xiu, CHEN Zhen-dong, GAO Hai-chou, HUANG De-gui 2006, 35(01): 29-32.  Abstract ( )   PDF (185KB) ( )   Effects of plant growth regulators on multiplication of cespitose buds from Asparagus officinalis new species ‘Jinling’ were studied.The result indicated that the admixture of NAA, 6-BA and KT gave good result for multiplication of cespitose buds.Taking the stem apex as explant, after 30 d, there were 26.3 cespitose buds per bottle when cultured in MS+NAA 0.1 mg/L+6-BA 0.2~0.3 mg/L+KT 0.1 mg/L;taking the mid stem as explant, after 30 d, there were 33.5 buds per bottle when cultured in MS+NAA 0.1 mg/L+6-BA 0.3~0.4 mg/L+KT 0.1 mg/L;taking the stem base as explant, there were 43.1 buds per bottle when cultured in MS+NAA 0.1 mg/L+6-BA 0.3~0.5 mg/L+KT 0.1 mg/L.The stem base was the optimum explant for rapid propagated. Related Articles | Metrics

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Substrate Selection for Biogas Fluid Soilless Culture of Cucumber YUAN Rui-fen 2006, 35(01): 33-34,41.  Abstract ( )   PDF (274KB) ( )   A comparative test of different substrates was conducted to select the optimum substrate for soilless culture of cucumber with 50% biogas fluid.The results showed that mixed substrate of rough river sand and sawdust (1:1) was best for the growth of cucumber. Related Articles | Metrics

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Structural Identification of Pumiloside, A Quinoline Alkaloid Glycoside, Isolated from Leaves of Davidio involucrata LIU Rong, OU'YANG Ming-an 2006, 35(01): 35-38.  Abstract ( )   PDF (618KB) ( )   A glycoside was isolated from the leaves of Davidio involucrata.NMR and MS methods were used to elucidate its chemical structure.The compound was determined as pumiloside, a quinoline alkaloid glycoside, and it was a biosynthetic precursor of camptothecin.The alkaloid glycoside was isolated from Davidio involucrata for the first time. Related Articles | Metrics

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Identification of Main Pigments in Red-fleshed Sweet Pomelo LU Xiu-min, LIU Qing, XU Juan, HUANG Xin-zhong, JIN Guang, LU Xin-kun, CHEN Xiao-ming 2006, 35(01): 39-41.  Abstract ( )   PDF (290KB) ( )   The Red-fleshed Sweet Pomelo is a mutant strain from Guanxi Sweet Pomelo.The character of the red mutant is stable after growing for several years.Two main pigments lycopene[(55.45 ±1.13) μg/g·dw] and β-carotene[(41.10 ±2.24) μg/g·dw] were identified in red-flesh sweet pomlo by using HPLC.The contest of xanthophyll in the juice sac was very low. Related Articles | Metrics

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Studies on the Optimum Operation Parameters of Freeze-Drying Banana CHEN Yi-nan 2006, 35(01): 42-45.  Abstract ( )   PDF (265KB) ( )   The comparative experiment was conducted on the operation parameters such as the material thickness, the freezing methods, the temperature of heating plate, the vacuum in processing technique of freeze-drying bananas.The results showed that the operation parameters had remarkable influences on both quality and output of freeze-drying bananas.The quick freezing method of bananas, material thickness of 5mm~7mm, heating temperature of 45℃, and 20~30 Pa vacuum for drying chamber were optimum technical conditions for drying of bananas. Related Articles | Metrics

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Utilization of Wild Rice High Protein Germplasm in Rice Breeding CHEN Cheng-bin 2006, 35(01): 46-51.  Abstract ( )   PDF (241KB) ( )   The necessity, significance and feasibility of utilization of wild rice high protein germplasm in rice breeding were studied.It also suggested strengthening special rice breeding and molecular genetics study of wild rice high protein germplasm. Related Articles | Metrics

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An Investigation on Poison Garden Plants in 7 Cities of China ZHAO Hong 2006, 35(01): 52-56.  Abstract ( )   PDF (216KB) ( )   Based on the investigation in 7 cities, i.e., Beiging, Shanghai, Hangzhou, Jinan, Qingdao, Shijiazhang and Weihai, the poison plants in urban landscape, about 146 species, 59 families are reported.They are about 10% of landscape plants.The reason of poisoning is analyzed and the culture state, toxic position and intensity of poison plants are investigated, and some suggestions for cultivation and management of poison plants are also put forward. Related Articles | Metrics

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Floristic Analysis of Seed Plants in Longhushan Nature Reserve, Guangxi Province LIN Hai-bo, TANG Shao-qing 2006, 35(01): 57-59.  Abstract ( )   PDF (162KB) ( )   The flora of seed plants in Longhushan Nature Reserve in Guangxi was analyzed in this paper.There are 851 species, which belong to 536 genera in 136 families in this reserve.At family level, the proportion of the widespresa type is 25.0%, the tropic type is 59.6%, and the temperate type is 15.4%.At genus level, the proportion of the tropic type is 80.6%, the temperate type is 18.4%, and the endemic to China type is 1.0%.The flora of seed plants in Longhushan Nature Reserve shows clear characteristics of tropic and Karst flora.It has the features of richness in species. Related Articles | Metrics

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The Features of Zhejiang Ligneous Flora and Its Relations with Plant Introduction and Domestication TANG Yu-li, ZHANG Yin-ke, LI Nian-lin, BAO Zhi-yi 2006, 35(01): 60-63.  Abstract ( )   PDF (243KB) ( )   The features of Zhejiang ligneous flora were investigated and the relationship between Zhejiang ligneous flora and plant introduction and domestication were also discussed.. Related Articles | Metrics

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Tissue Culture and Rapid Propagation of Plectranthus hilliardiae hybrid HU Bo, CHEN Gang, Birts G, LI Ling 2006, 35(01): 64-64,63.  Abstract ( )   PDF (226KB) ( )   Related Articles | Metrics

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Changes of Ursalic Acid Content in Clerodendranthus spicatus during Different Growing Period ZHANG Ping, LUO Guan-xing, TIE Wan-zhu, LAN Shi-kuan 2006, 35(01): 65-65,74.  Abstract ( )   PDF (130KB) ( )   Related Articles | Metrics

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Tissue Culture of Photinia frascri ‘Robusta’ WANG Hai-qin, FENG Xian-ju, LUO Jun-qin 2006, 35(01): 66-66.  Abstract ( )   PDF (93KB) ( )   Related Articles | Metrics

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Adaptable response to low-temperature stress and signal transduction of post-harvest fruit TAN Wei-ping, CHEN Jian-ye, ZHANG Hui, ZENG Ji-wu 2006, 35(01): 67-70.  Abstract ( )   PDF (192KB) ( )   The adaptable response to low-temperature stress of post-harvest fruit are reviewed.The signal transduction of fruit under low temperature are also discussed.It shows that molecular biology and gene engineering provide an effective approach to further study. Related Articles | Metrics

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Progress in Tissue Culture of Phalaenopsis spp. ZHENG Yu-zhong, ZHANG Zhen-xia, CHEN Ze-hua 2006, 35(01): 71-74.  Abstract ( )   PDF (187KB) ( )   The research progress of tissue culture of Phalaenopsis, including the effects of explants sources, culture mediums, hormones and nutrition compositions on multiplication and differentiation of protocorm-like body, the inhibition on browning of explants and methods of rooting and seedling strengthening before transplanting, are reviewed.Some reference materials are also provided for further study in tissue culture of phalaenopsis. Related Articles | Metrics

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A Review of Advance in Gene Engineering in Lily LI Hong-yu, MA Hong-xiang, LEI Jia-jun, YU Gui-hong, LU Wei-zhong, ZHOU Miao-ping, ZHOU Jian-tao 2006, 35(01): 75-80.  Abstract ( )   PDF (258KB) ( )   New advances in gene engineering researches of lily, including gene clone, gene transformation method and application in lily, are reviewed.Meanwhile, the existing problems and prospects of the application of modern biotechnology in lily are discussed. Related Articles | Metrics

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A Review of Molecular Mechanism during the Formation of Fruit Quality PAN Teng-fei, LI Yong-yu, QIU Dong-liang 2006, 35(01): 81-84.  Abstract ( )   PDF (191KB) ( )   The researches on molecular mechanism during the formation of fruit quality, including the relative enzymes and their gene expression in sugar metabolism and organic acid metabolism are reviewed.The formation of color in fruits also discussed. Related Articles | Metrics