Abstract: The browning ratio of Phalaenopsis sp.leaf explant in vitro growing on MS liquid paper bridge and 1/2 MS after 10 d is 0 and 67%, respectively, less than that growing on MS, B₅ and N₆.Doubling the content of FeSO₄ or microelement deficiency could increase the browning rate of explant cultured in MS medium.Reducing the content of agar could increase the degree of browning.The higher the intensity of light, the more serious the explant browning.Explant cultured on medium supplemented with citric acid showed a considerable reduction in browning.Adding Vit C, active carbon or PVP could not overcome the browning of explant.

Key words: phalaenopsis sp., explant, browning, medium, light

摘要： 蝴蝶兰叶片外植体在MS纸桥培养基上培养10 d发生褐变率较低,1/2 MS次之,MS、B₅和N₆培养基外植体褐变率最高。MS培养基中铁盐加倍或微量元素缺乏造成外植体严重褐变,培养基积累很多褐色物质;减少琼脂含量,外植体褐变加重;添加柠檬酸可减轻外植体褐变;而添加活性炭、抗坏血酸或PVP对减轻外植体褐变无显著作用。光照强度增加外植体褐变严重。

关键词: 蝴蝶兰, 外植体, 褐变, 培养基, 光照