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10 June 2004, Volume 33 Issue 02 Previous Issue    Next Issue

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Isolation and sequence analysis of LEAFY homologous gene from mango HU Gui-bing, LIN Shun-quan, YE Zi-xing, XU Chang-jie, ZHANG Shang-long 2004, 33(02): 1-4.  Abstract ( )   PDF (151KB) ( )   Analyzing a conserved sequence of the LEAFY (LFY) gene, which plays an important role in floral development, a pair of 23 bp primers was used to amplify an 822 bp fragment by polymerase chain (PCR) with genomic DNA of mango. The fragment was cloned into pGEM-T Easy vector, and then sequenced. A fragment in the 3'-end of LFY homologous gene named miLFY gene was obtained. The result of sequence analysis indicated that there was an intron of 415 bp in the fragment, and the exons encoded 135 amino acids. The miLFY gene has been registered in GenBank with the accession number AY189684. After the deduced amino acid sequence of the fragment was submitted to GenBank and blast with LFY homologous gene of other plants, it was found that the homology reached 74%~97%. This result suggested that miLFY gene might have the same function as other LFY homologous gene. Related Articles | Metrics

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Effects of drought stress on tobacco photosynthesis in vigorous growing period QIN Peng, YANG Zhi-wen, KONG Zhi-you, LIU Feng-ying, LIU Fei-hu 2004, 33(02): 5-7.  Abstract ( )   PDF (103KB) ( )   Effects of drought stress on photosynthesis of two pot-culture tobacco cultivars (K326 and Hongda) were studied. The results showed that net photosynthetic rate (Pn), stomatal conductance (Gs) and transpiration rate (Tr) decreased under drought stress; intercellular CO2 concentration (Ci) decreased under slight drought stress and increased under severe drought stress (as the drought time was prolonged). The photosynthesis of K326 decreased slighter and later than that of Hongda. The experiment indicated that tobacco photosynthesis was sensitive to drought stress and the drought-resistant cv. K326 had less injury than non-drought-resistant cv. Hongda under drought stress. Related Articles | Metrics

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Effects of IBA and NAA on POD and PPO in the development of adventitious root of Elaeocarpus sylvestris GU Yun-jie, DENG Xiao-min, ZHANG Jian-kang, SHI Da-xing, WANG Mi-li 2004, 33(02): 8-9,12.  Abstract ( )   PDF (141KB) ( )   The activities of peroxidase and polyphenol oxidase in the development of adventitious root of Elaeocarpus sylvestris were studied. The results showed that after treated with IBA and NAA, the rate of root formation was high; the peroxidase activity in adventitious root development decreased rapidly in 0~4days and then increased; when roots began elongating, the peroxidase activity decreased; no roots were visible without hormones; the peroxidase activity in the adventitious root development in 0~16 days increased and then decreased; The polyphenol oxidase activity was very low during the adventitious root development. Related Articles | Metrics

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Chemical constituents in roots of Polygonum perfoliatum WANG Ding-yong, LU Jiang-Hong 2004, 33(02): 10-12.  Abstract ( )   PDF (136KB) ( )   Seven compounds were isolated from the roots of Polygonum perfoliatum. By combination of chemical methods and spectral analyses, structures, these compounds were identified as β-sitosterol (1), trans-p-hydroxycinnamic acid (2), quercetin (3), quercetin-3-O-β-D-glucoside (4), emodin (5), physcion (6) and aloeemoclin (7). All these compounds were obtained from this plant for the first time. Related Articles | Metrics

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Effects of a new preservatives for cut Anthurium GUO Zhao-wu, ZOU Ying-bin, XIA Shi-tou 2004, 33(02): 13-17.  Abstract ( )   PDF (135KB) ( )   Through two-year studies, a new preservative for cut Anthurium was developed. This preservative is nonpoisonous,corrosion-free, colorless and pollution-free and has remarkable preservative effect. It was proved that this preservative had steady preservative effect under different environmental temperatures through comparative experiments; and could prolong preservative time and hold significant advantage in comparison with Ag+agent and the control. It has a bright prospect for further development. Related Articles | Metrics

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The process of meiosis and genesis of microspore in Heptacodium miconioides ZHANG Yue-qin, XU Dong-qing, CAI Jie-jie 2004, 33(02): 18-20.  Abstract ( )   PDF (365KB) ( )   The meiosis and the formation of microsporogenesis of Heptacodium miconioides was studied. The meiosis of microspore mother cells is of simultaneous type. The arrangement of microspores in tetrad is tetrahedral and sometimes decussate or isobilateral. The development of anther walls belongs to dicotyledonous type. Related Articles | Metrics

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The properties and the extraction technology of red pigment from Syzygium buxifolium fruits HUANG Xiao-dong, LIU Jian-qiu 2004, 33(02): 21-24.  Abstract ( )   PDF (101KB) ( )   The physical and chemical properties of red pigment from Syzygium buxifolium fruits and the optimal extraction conditions of pigment by the orthogonal experiment were studied. The results showed that the pigment is anthocyanin, which optimal conditions of extraction were as follows:extraction agent of 1%HCL-ethanol(1ml HCl:99ml 95%ethanol), ratio of sample weight(g) to solvent volume(ml) 1:10, each extraction for three hours at 35℃ and by three times. Related Articles | Metrics

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Effects of food colors on dyeing of cut chrysanthemum ZHANG Yu-ping, HUANG Yun-feng, ZHANG Fang 2004, 33(02): 25-27.  Abstract ( )   PDF (99KB) ( )   The effects of food colors on dyeing of cut chrysanthemum (Dendranthema grandifloram) were studied. The results showed that Brilliant Blue and Tartrazine were more suitable for cut chrysanthemum than Ponceau 4R and Amaranth; Grape Violet and Milkchocolate Brown were not suitable for cut chrysanthemum. The suitable dyeing density and time for different food colors were Brilliant Blue(6g/L, 4h), Tartrazine(6g/L, 4h), Ponceau 4R(12g/L, 6h), Amaranth(9g/L, 6h), Grape Violet (20g/L, 8h) and Milkchocolate Brown(20g/L,8h), and the optimal value were 6.6,10.9,10.9,10.2,10.3 and 10.4, respectively. The dyeing speed was fast when temperature was higher and cut chrysanthemum opened in a great degree. The dyeing effects were not significant for the stems with more or less leaves. The vase life became shorter after dyeing but still had 5~7 days for ornament. Related Articles | Metrics

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Early evaluation on fast-growth and stability of introduced Pinus taedam families SUN Xiao-xia, LIANG Yi-chi, RUAN Shao-ning 2004, 33(02): 28-31,24.  Abstract ( )   PDF (171KB) ( )   In comparison with slash pine, which was introduced earlier from abroad, and the native mason pine, the five-year-old loblolly pine (Pinus taedam) families, which was introduced into Fujian Province, were evaluated. The results indicated that there were significant or great significant differences in families, gene and site mutual variance. Loblolly pine grew more quickly than slash pine and mason pine. By synthesizing clustering analysis, 25 loblolly pine families were divided into 4 groups, i. e. best, better, moderate and poor. Compared to mason pine, the actual gains of loblolly pine families were 83.2%, 63.4%, 48.5% and 33.7%, respectively. Using gene grouping means to evaluate the fast-growth and stability of loblolly pine families, No. 19, 25, 18, 21 and 10 families had superior gene type, which grew fast and varied little. Related Articles | Metrics

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The impact of different reproduction ways on the growth of forest vegetation and soil properties HUANG Zi-liang 2004, 33(02): 32-35.  Abstract ( )   PDF (159KB) ( )   The impacts of three reproduction ways on forest vegetation in natural secondary forest slashing were studied. The results indicated that artificial forestation destroyed the forest vegetation and soil, resulting the species loss, soil and water loss and soil fertility decline, unfavorable for the forest growth. The species abundant degree in different stands was:natural regeneration >artificial regeneration >artificial afforestation; the vegetation biomass under woods was artificial regeneration >natural regeneration >artificial afforestation. Natural regeneration and artificial regeneration were beneficial to protecting the species variety and the vegetative growth, as well as the improvement of the physical and chemical properties in soil and increase in soil fertility. As a result, the higher economic benefits could be obtained. Related Articles | Metrics

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Introduction of Vaccinium ashei in Guizhou Province LI Shun-qin, NIE Fei, LIAO You-jiang 2004, 33(02): 36-38,43.  Abstract ( )   PDF (163KB) ( )   Six cultivars of Vaccinium ashei were grown on acid yellow soil, which were introduced from Jiangsu Institute of Botany in March 2000. All cultivars grew well and began to come into blossom and bear fruit in the following year. Individual weight of fruit was 0.5~2.0g, total sugar 8.9%~9.4%, total acid 0.06%~0.19%. The contents of elements, such as Ca, K, P, Fe and Zn, Vitamin A, E and C were relatively high. All cultivars had good adaptability, suitable for growing on acid yellow soil in hilly land in Guizhou Province. Related Articles | Metrics

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Investigation on natural development of arbuscular mycorrhiza in citrus trees WU Qiang-sheng, ZHOU Kai-bing, XIA Ren-xue, HUANG Ren-hua, HU Li-ming 2004, 33(02): 39-40.  Abstract ( )   PDF (96KB) ( )   Natural development of arbuscular mycorrhiza in citrus trees was investigated at three sample plots of Citrus Demonstration Farm, Sanxihe and Dengjiapo in Zhigui County, Hubei Province. The results indicated that all the investigated citrus trees were infected by arbuscular mycorrhiza fungi and the development of arbuscular mycorrhiza varied with the citrus varieties and localities. The highest infected percentage of Trifoliate/Robertson No.36/Kate occurred at the Citrus Demonstration Farm, and also the highest spore density of Trifoliats/Robertson No.36/Washington sanguin. No significant correlation exhibited between mycorrhizal infection and spore density. Related Articles | Metrics

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Nutritional requirement of growth of Lactarius deliciosus hypha LI Wen-yi, LIU Jian-cheng 2004, 33(02): 41-43.  Abstract ( )   PDF (102KB) ( )   Lactarius deliciosus is one of the valuable edible fungi, which has developmental potential. The nutritional requirement of growth of Lactarius deliciosus hypha was studied. The results showed that the best origin of carbon for its growth was sucrose; the best origin of nitrogen was protein; trace elements inhibited its growth and vitamins had little inhibition on its growth. Related Articles | Metrics

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Rapid propagation of Mozzie buster LU Bing-guo, CAO Zhi, CHEN Zhao 2004, 33(02): 44-46.  Abstract ( )   PDF (130KB) ( )   Taking the petioles and leaves of Mozzie buster as explants, tissue culture and rapid propagation were studied. The results showed that the leaves cultured on MS media supplemented with 6-BA 2.0mg/L and NAA 0.5mg/L was best for inducing callus and adventitious buds, and MS media with 6-BA 1.0mg/L and NAA 0.3mg/L was suitable for subculture of adventitious buds. The microshoots rooted in low salt media containing low level of auxin, rooting rate up to 100%. Related Articles | Metrics

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Plant design in forest garden LIANG Yun, LIU Yan 2004, 33(02): 47-50.  Abstract ( )   PDF (155KB) ( )   Based on the present analysis of the plant design in the plan of forest garden, and according to the different requirement of different senses, a general technique of plant design in the forest garden is proposed. Related Articles | Metrics

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Rare and endangered plants in ecology public good forest around Ansha Reservoir,Yong'an,Fujian LIN Kui 2004, 33(02): 51-54,50.  Abstract ( )   PDF (181KB) ( )   A preliminary investigation indicated that there exist 21 species of rare and endangered plants under 19 genera of 14 families in ecology public good forest around Ansha Reservoir, Yong'an City, Fujian Province. Among them, 1 species belong to the grade one of national protected plants and 7 species the grade two. The resources of the rare and endangered plants possess the features of richness in variety and composition, obvious tropical nature, antiquity of origin, abundance of endemic elements, diversity of usage and greatness of value. Related Articles | Metrics

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Plant resources on Wanshan Islands in Zhuhai City,Guangdong Province HE Zhong-jian, FENG Zhi-jian, LI Zhen-kui 2004, 33(02): 55-59.  Abstract ( )   PDF (126KB) ( )   There exist 972 species including 84 cultivars belonging to 547 genera of 176 families of vascular plants on Wanshan Islands, Zhuhai City, Guangdong Province. Among them, 35 species under 27 genera of 21 families are ferns; 11 species under 8 genera of 6 families are gymnospermae and 926 species under 510 genera of 139 families are angiosperm.11 species are the grade two of national protected wild plants. 4 species are the rare and endangered plants in Guangdong Province. 492 species are medicinal plant, 109 species timer plant, 143 species ornamental plant, 21 species forage plant, 105 species fiber plant, 62 species starch plant, 81 species oil plant, 72 species of scent plant and 58 species tanning plant, as well as some species of miscellaneous usages. Related Articles | Metrics

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Medicinal Ficus resources in Xishuangbanna,Yunnan Province ZHANG Li-xia, GUAN Zhi-bin 2004, 33(02): 60-62.  Abstract ( )   PDF (125KB) ( )   Species distribution, habitat, curative effect of the medicinal Ficus and their utilization in Xishuangbanna, Yunnan Province are reviewed. Some suggestions on their development and utilization are also presented. Related Articles | Metrics

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Tissue culture of Musa AAB Group Fenjiao LIN Jiang-bo, ZHANG Shu-he, GAN Yong-hui, LIN Jia-geng 2004, 33(02): 63-63.  Abstract ( )   PDF (57KB) ( )   Related Articles | Metrics

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A review of application of transgenic techniques on orchids YANG Cui-qin, LIU Wei, WANG Sheng-bin, YE Qing-sheng 2004, 33(02): 64-68.  Abstract ( )   PDF (161KB) ( )   The advances in research on applied transgenic techniques, transferred target genes, screening and assay of transgenic plants in transformation of orchids are reviewed in this paper. Related Articles | Metrics

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A review of plant chromosome microdissection technique and its applications HE Feng-hua 2004, 33(02): 69-72.  Abstract ( )   PDF (124KB) ( )   The principle and methods of plant microdissection and microcloning are introduced in detail, and their applications in the field of botany are summarized. Related Articles | Metrics

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A review of advances in research on Dendrobium nobile WANG Lin, YE Qing-sheng, LIU Wei 2004, 33(02): 73-76.  Abstract ( )   PDF (161KB) ( )   Dendrobium nobile has both ornamental and pharmaceutical values. The botanical characters, cultural practices, medicinal constituents, pharmacological activities and economic values are reviewed in this paper. Related Articles | Metrics

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A review of advances in active oxygen metabolism in plants under water stress WU Zhi-hua, ZENG Fu-hual, MA Sheng-jian, XIE Yao-jian, LU Xiang-yang 2004, 33(02): 77-80.  Abstract ( )   PDF (155KB) ( )   The advance of AOS and its relationship with drought (water stress) tolerance of plants under water stress are reviewed, with great emphasis on the function and mechanism of detoxic enzymes and antioxidants in water stress response. In addition the development of transgenic plants is also discussed. Related Articles | Metrics