亚热带植物科学

亚热带植物科学 ›› 2011, Vol. 40 ›› Issue (01): 41-44.DOI: 10.3969/j.issn.1009-7791.2011.01.011

• 研究报告 • 上一篇    下一篇

KT和NAA对黄芪带芽茎段增殖、愈伤诱导和生根的影响

尹明华,曹振龙   

  1. 上饶师范学院 生命科学学院, 江西 上饶 334001
  • 收稿日期:2010-11-01 出版日期:2011-03-10 发布日期:2011-03-10
  • 作者简介:尹明华(1973-),女,江西吉安人,讲师,硕士,从事生物技术研究。
  • 基金资助:

    江西省教育厅科技一般项目(GJJ09374);2009年江西省高校省级教改立项项目(JXJG-09-16-10)

The Effects of KT and NAA on Proliferation of Astragalus membranceus Stem with a Bud and its Induction of Callus and Rooting

YIN Ming-hua, CAO Zhen-long   

  1. College of Life Sciences, Shangrao Normal University, Shangrao 334001, Jiangxi China
  • Received:2010-11-01 Online:2011-03-10 Published:2011-03-10

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摘要： 通过建立组织培养快繁体系,探讨KT和NAA对膜荚黄芪茎段诱导生成新生茎、愈伤组织及生根的影响。KT/NAA值为4时,对茎的诱导作用明显,获得最多的茎平均数(11.7条)。NAA促进愈伤组织形成,在1 mg/L NAA作用下最大的平均直径为1.4 cm,多为白色。根生于白色愈伤组织,并受NAA促进、KT抑制。1 mg/L NAA对根数、根长和次级根数均有促进作用,但对根粗有抑制作用。

关键词: 荚膜黄芪, KT, NAA, 组织培养

Abstract: Through the establishment of tissue culture rapid propagation system, the effects of KT and NAA on the induction of new stem, callus and roots of Astragalus membranceus were studied.The induction of new stem was related to the ratio of KT/NAA, which was obvious with the average number of new stem was the maximum, 11.7, when the value was 4.With the promotion of NAA, lots of white callus was produced.The average diameter of white callus, which was in the medium with 1 mg/L of NAA, was 1.4 cm.Roots came mainly from the white callus, being promoted by NAA but inhibited by KT.1 mg/L of NAA played a key positive role in the increase of roots number, the average length of roots and the number of secondary roots, but negative in roots average diameter.

Key words: Astragalus membranceus, KT, NAA, tissue culture

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