一种简便高效的苜蓿半湿扦插繁殖方法

doi:10.3969/j.issn.1009-7791.2024.04.005

亚热带植物科学 ›› 2024, Vol. 53 ›› Issue (4): 325-330.DOI: 10.3969/j.issn.1009-7791.2024.04.005

一种简便高效的苜蓿半湿扦插繁殖方法

田代财1,3,4，胡锦宁2,3,4，丛丽丽1,3,4，柴茂峰1,3,4，王增裕1,3,4，谢红丽1,3,4*

(1. 青岛农业大学草业学院，山东青岛 266109；2. 青岛农业大学动物科技学院，山东青岛 266109；3. 黄河三角洲草地资源与生态国家林业和草原局重点实验室，山东青岛 266109；4. 青岛市滩涂盐碱地特色植物种质创制与利用重点实验室，山东青岛 266109)

收稿日期:2024-07-30 接受日期:2024-08-10 发布日期:2024-11-20
通讯作者: 谢红丽
基金资助:
国家重点研发(2023YFF1001400)；国家自然科学基金面上项目(32371763)；山东省自然科学基金面上项目(ZR2023MC120)

A Simple and Efficient Method of Alfalfa Propagation by Semi-wet Cutting

TIAN Dai-cai1,3,4, HU Jin-ning2,3,4, CONG Li-li1,3,4, CHAI Mao-feng1,3,4, WANG Zeng-Yu1,3,4, XIE Hong-li1,3,4*

(1. College of Grassland Science, Qingdao Agricultural University, Qingdao 266109, Shandong China; 2. College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, Shandong China; 3. Key Laboratory of Grassland Resources and Ecology of the State Forestry and Grassland Bureau of the Yellow River Delta, Qingdao 266109, Shandong China; 4. Key Laboratory of Plant Germplasm Creation and Utilization of Characteristic Plants in Saline and Alkaline Land of Qingdao Municipality, Qingdao 266109, Shandong China)

Received:2024-07-30 Accepted:2024-08-10 Published:2024-11-20
Contact: XIE Hong-li

摘要/Abstract

摘要： 扦插是良种扩繁及资源保存的重要手段之一，对于自交不亲和、异花授粉的紫花苜蓿来说尤其重要。目前已有的苜蓿扦插方法在应用中还存在一定的局限性，如操作复杂、周期较长等问题。本研究依据线粒体调控根再生理论提出了一种简便高效的新型苜蓿扦插方法—半湿法扦插。本实验选取了‘中苜一号’与‘新疆大叶’两种常用紫花苜蓿品种，用保湿材料将插穗覆盖后放置于简易容器中，同时评估了半湿法与水培法扦插下的生根效果及两种扦插方法的差异。结果表明，半湿法扦插在两个不同品种背景下的生根率没有明显差异，生根效率都非常高，第8天生根率超过90%，到第10天接近100%，且显著高于水培扦插。综上所述，半湿法扦插不仅简化了扦插的操作流程，还有效地缩短了生根时间，对苜蓿的扩繁及野外资源采集具有重要的实际应用价值。

关键词: 半湿法；苜蓿扦插；生根率

Abstract: Cutting is one of the important methods to propagate and preserve good varieties and resources, especially for self-incompatibility and cross-pollination alfalfa. Currently, there are some limitations in the application of current alfalfa cutting methods, such as complicated operation and long cycle. Based on the theory of root regeneration controlled by mitochondria, a simple and efficient new cutting method of alfalfa, named semi-wet cutting method was proposed in this study. This method can be completed only by covering the cuttings with moisturizing material and placing them in a container. In this experiment, two alfalfa varieties, ‘Zhongmu No. 1’ and ‘Xinjiang Daye’, were selected and cultured by semi-wet method and hydroponics method, aiming at comparing the rooting effect of different varieties under semi-wet method and the difference between the two cutting methods. The experimental results showed that there was no significant difference in the rooting rate of the two varieties by using semi-wet cutting, and the rooting rate was very high, more than 90% on the 8th day and close to 100% on the 10th day in both varieties, which was significantly faster than that by using hydroponic cutting. To sum up, semi-wet cutting not only simplified the operation process, but also effectively shortened the rooting time, which has important practical application value for the propagation of alfalfa and the collection of field resources.

Key words: semi-wet method; alfalfa cutting; rooting rate

中图分类号:

Q945.5

田代财，胡锦宁，丛丽丽，柴茂峰，王增裕，谢红丽. 一种简便高效的苜蓿半湿扦插繁殖方法[J]. 亚热带植物科学, 2024, 53(4): 325-330.

TIAN Dai-cai, HU Jin-ning, CONG Li-li, CHAI Mao-feng, WANG Zeng-Yu, XIE Hong-li. A Simple and Efficient Method of Alfalfa Propagation by Semi-wet Cutting[J]. Subtropical Plant Science, 2024, 53(4): 325-330.

参考文献

[1] 梁晓斐, 刘文亮, 刘新鹏, 张宇, 李国鹏, 于洪柱. 苜蓿扦插关键技术[J]. 江西畜牧兽医杂志, 2022(5): 32–33.
[2] 刘青松, 肖宇, 徐玉鹏, 阎旭东. 不同处理方式对10个苜蓿品种扦插成活率的影响分析[J]. 中国草地学报, 2018, 40(5): 109–113.
[3] 牛山. 影响苜蓿扦插成活因素的研究[D]. 咸阳: 西北农林科技大学硕士学位论文, 2007.
[4] 牛山, 韩清芳, 贾志宽. 不同处理对苜蓿扦插生根能力的影响[J]. 西北农业学报, 2007(1): 149–152.
[5] 赵丽, 柴小琴, 刘娟. 紫花苜蓿扦插繁殖技术要点[J]. 甘肃农业科技, 2012(3): 57–58.
[6] 王文涛, 杨志敏, 王运涛, 张宝英, 邢向荣. 紫花苜蓿扦插繁殖技术要点[J]. 现代农村科技, 2013(17): 14–14.
[7] 刘琼琼, 贾志宽, 韩清芳, 王红艳. 不同多叶型苜蓿单株扦插后生物学性状的比较[J]. 西北农林科技大学学报(自然科学版), 2006(5): 39–44.
[8] 黄新育, 柴华, 李红, 杨伟光, 杨曌, 木黑提?托里别根, 木热力?巴格孜. 大田条件下不同处理对多叶型苜蓿扦插效果的影响[J]. 现代畜牧科技, 2015(3): 34–35.
[9] 杨婕, 石凤翎. 不同基质对不同苜蓿种质材料扦插枝条再生长的影响[J]. 草原与草业, 2020, 32(2): 18–21.
[10] 洪汉辉, 康向阳, 汪晓峰. 年龄效应对白杨硬枝扦插苗生长及其茎皮部解剖结构和叶片生化指标的影响[J]. 西北植物学报, 2018, 38(2): 274–281.
[11] Zhao Z, Zhang W, Liu Y, Li S, Wu Y, Sun X, Li S, Ma L, Sun J, Yang Q, Li Y, Yang G, Wang Z Y, Cong L. De novo hydroponics system efficiency for the cuttings of alfalfa (Medicago sativ L.) [J]. Physiology and Molecular Biology of Plants, 2021, 27(6): 1413–1421.
[12] 丛丽丽, 张文玉, 李帅, 吴垚, 孙晓晖, 李思玉, 刘扬, 王宗宇, 杨国锋, 孙娟, 栗振义. 一种紫花苜蓿水培扦插扩繁方法[P]. 中国: CN202010045389.7, 2022-01-28.
[13] 敖红, 王昆, 冯玉龙. 长白落叶松插穗的内源激素水平及其与扦插生根的关系[J]. 植物研究, 2002(2): 190–195.
[14] Legue V, Rigal A, Bhalerao R. Adventitious root formation in tree species: involvement of transcription factors [J]. Physiologia Plantarum 2014, 151: 192–198.
[15] Xu L, Huang H. Genetic and epigenetic controls of plant regeneration [J]. Current Topics in Developmental Biology, 2014, 108: 1–33.
[16] Xu C, Tao Y, Fu X, Li G, Xing H, Li C, Yang Z, Su H, Wang X, Hu J, Fan D, Chiang V L, Luo K. The microRNA476a-RFL module regulates adventitious root formation through a mitochondria–dependent pathway in Populus [J]. New Phytologist, 2021, 230(5): 2011–2028.
[17] Pacurar D I, Perrone I, Bellini C. Auxin is a central player in the hormone cross– talks that control adventitious rooting [J]. Physiologia Plantarum, 2014, 151(1): 83–96.
[18] Trupiano D, Yordanov Y, Regan S, Meilan R, Tschaplinski T, Scippa G S, Busov V. Identification, characterization of an AP2/ERF transcription factor that promotes adventitious, lateral root formation in Populus [J]. Planta, 2013, 238(2): 271–282.
[19] Hobbie L, Estelle M. The axr4 auxin-resistant mutants of Arabidopsis thaliana define a gene important for root gravitropism and lateral root initiation [J]. The Plant Journal, 1995, 7(2): 211–220.
[20] Rigas S, Debrosses G, Haralampidis K, Vicente-Agullo F, Feldmann K A, Grabov A, Dolan L, Hatzopoulos P. TRH1 encodes a potassium transporter required for tip growth in Arabidopsis root hairs [J]. The Plant Cell, 2001, 13(1): 139–151.
[21] Quan J, Zhang S, Zhang C, Meng S, Zhao Z, Xu X. Molecular cloning, characterization and expression analysis of the SAMS gene during adventitious root development in IBA- induced tetraploid black locust [J], PloS One, 2014, 9(10): e108709.
[22] Dash G K, Senapati S K, Rout G R. Effect of auxins on adventitious root development from nodal cuttings of Saraca asoka (Roxb.) de Wilde and associated biochemical changes [J]. Journal of Horticulture and Forestry, 2011, 3: 320–326.

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一种简便高效的苜蓿半湿扦插繁殖方法

A Simple and Efficient Method of Alfalfa Propagation by Semi-wet Cutting

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