关键词: 草酸氧化酶, 筛选, 性质

Abstract: 88 plant species from 44 families were used as raw material to screen oxalate oxidase (OXO), from which, 60 species from 34 families showed detectable OXO activities, and 30 species showed quite high OXO activity amounting to >0.02 U/mg protein, and >0.2 U/g·fw. In order to evaluate the possibility of scavenging oxalate in mammalian body by OXO, five plant species with high biomass and OXO activity, namely, Echinacea purpurea, Patrinia villosa, Sarcandra glabra, Rhinacanthus nasutus and Glycyriza uralensis were chosen as candidates to study their characteristics. The OXOs from the above 5 plant species were all stable at high temperature and acid environment, and those from Echinacea purpurea, Rhinacanthus nasutus, and Patrinia villosa were not inhibited by NaCl and CaCl₂ or hydrolysised by pepsin, indicating a high application potential. The enzyme from Glycyriza uralensis was not inhibited by NaCl and CaCl₂ but sensitive to pepsin, while that from Sarcandra glabra was inhibited by NaCl and CaCl₂ as well as pepsin.

Key words: oxalate oxidase, screening, characteristic