亚热带植物科学 ›› 2017, Vol. 46 ›› Issue (01): 25-29.DOI: 10.3969/j.issn.1009-7791.2017.01.005

• 植物生理生化与分子生物学 • 上一篇    下一篇

武夷山及周边地区黄精植物ISSR分子标记鉴定及HPLC指纹图谱研究

杨青,高华春,陈红丽,陈全成,徐鲜钧   

  1. (1.武夷山生物研究所,福建 武夷山 354300;2.厦门大学药学院,福建 厦门 361102)
  • 收稿日期:2016-12-30 修回日期:2017-02-16 出版日期:2017-03-30 发布日期:2017-03-30
  • 通讯作者: 杨青
  • 作者简介:杨青,硕士,高级农艺师,从事生物多样性及生物资源开发利用研究。
  • 基金资助:

    福建省科技厅自然科学基金重点项目(2013Y0018);福建省科技厅自然科学基金青年项目(2015J01065)

Identification of Polygonatum from Wuyishan and Its Surrounding Areas by ISSR Molecular Markers and Their HPLC Fingerprints

YANG Qing,GAO Hua-chun,CHEN Hong-li,CHEN Quan-cheng,XU Xian-jun   

  1. (1.Wuyishan Institute of Biology, Wuyishan 354300, Fujian China; 2.School of Pharmaceutical Sciences, Xiamen University, Xiamen 361102, Fujian China)
  • Received:2016-12-30 Revised:2017-02-16 Online:2017-03-30 Published:2017-03-30
  • Contact: YANG Qing

摘要: 采用ISSR分子标记鉴定方法及HPLC色谱方法对武夷山及周边地区5份黄精植物样本进行DNA分子标记鉴定及指纹图谱分析,并通过聚类分析软件分别探讨其遗传相关聚类图谱。ISSR分子标记法能较好地对5份黄精植物样本进行区分,HPLC指纹图谱分析同样表明5份黄精样本化学成分存在一定的差别。DNA分子标记及HPLC指纹图谱分析的遗传相关聚类分析结果相似,表明武夷山及周边地区的野生黄精种质资源存在明显的差别。

关键词: 黄精, ISSR分子标记, HPLC 指纹图谱

Abstract: The purpose of this project was to identify and analyze the genetic relationships of 5 kinds of Polygonatum in Wuyishan and its surrounding areas. Using ISSR marker and HPLC molecular identification method for chromatographic methods analysis of DNA markers for identification and fingerprinting of 5 Polygonatum samples, the genetic correlation map of Polygonatum was studied by cluster analysis software. ISSR molecular marker method could be used to distinguish Polygonatum of Wuyishan and its surrounding areas. HPLC fingerprint analysis also showed that there were some differences in the composition of Wuyishan and its surrounding distribution. The results of genetic correlation cluster analysis were similar by ISSR and HPLC. There were obvious differences in the germplasm resources of Wuyishan and its surrounding areas from the DNA molecular markers and HPLC fingerprinting analysis results.

Key words: Polygonatum, ISSR molecular marker, HPLC fingerprint

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