亚热带植物科学 ›› 2017, Vol. 46 ›› Issue (4): 375-378.DOI: 10.3969/j.issn.1009-7791.2017.04.015

• 植物栽培与组培 • 上一篇    下一篇

独脚金离体培养与快速繁殖

蔡时可,魏杰,李战超,王继华   

  1. 1.广东省农作物遗传改良重点实验室,广东省农业科学院作物研究所,广东 广州 510640; 2.华南农业大学生命科学学院,广东 广州 510642
  • 收稿日期:2017-07-18 修回日期:2017-08-10 出版日期:2017-12-29 发布日期:2017-12-29
  • 通讯作者: 王继华
  • 作者简介:蔡时可,硕士,高级农艺师,从事药用植物学研究。

In vitro Culture and Rapid Propagation of Striga asiatica

CAI Shi-ke,WEI Jie,LI Zhan-chao,WANG Ji-hua   

  1. 1.Guangdong Key Laboratory for Crops Genetic Improvement, Crops Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong China; 2.College of Life Sciences, South China Agricultural University, Guangzhou 510642, Guangdong China
  • Received:2017-07-18 Revised:2017-08-10 Online:2017-12-29 Published:2017-12-29
  • Contact: WANG Ji-hua

摘要: 采用茎段作为外植体,建立独脚金Striga asiatica的组织培养快速繁殖方法。结果显示,0.1%升汞溶液处理7~9 min是较有效的消毒方法;诱导不定芽增殖培养基适宜配方为MS + 6-BA 3.0 mg·L-1 + IBA 0.1 mg·L-1;生根培养基配方为MS + NAA 0.1 mg·L-1 + IBA 0.5 mg·L-1,培育周期为3个月。

关键词: 独脚金, 组织培养, 快速繁殖

Abstract: Stems of Striga asiatica were used as the explants to establish optimum conditions for rapid propagation in vitro. The results showed that disinfecting the stems 7—9 min with 0.1% mercuric chloride solution was the best methods; MS medium with 3.0 mg·L-1 6-BA and 0.1 mg·L-1 IBA was optimum for inducing adventitious and proliferation reproduction. The suitable medium for rooting was MS + 0.1 mg·L-1 NAA + 0.5 mg·L-1 IBA.

Key words: Striga asiatica, tissue culture, rapid propagation

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